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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
13/12/2010 |
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Data da última atualização: |
26/08/2013 |
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Autoria: |
SEEHAUS, K.; TENHAKEN, R. |
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Afiliação: |
KAI SEEHAUS; RAIMUND TENHAKEN. |
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Título: |
Cloning of genes by mRNA differential display induced during the hypersensitive reaction of soybean after inoculation with Pseudomonas syringae pv. glycinea. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Plant Molecular Biology, v. 38, n. 6, p. 1225-1234, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean (Glycine max [L.] Merr.) cell suspension cultures (cv. Williams 82) inoculated with the pathogenic bacteria Pseudomonas syringae pv. glycinea respond with a hypersensitive reaction (HR) when the bacteria express the avirulence gene avrA. A mRNA differential display was established for this system to allow the identification of genes induced during the HR. Six PCR-fragments (DD1–DD6) from the differential display analysis were identified, which are induced during the HR. Database searches revealed that the fragment DD1 encodes chalcone isomerase and DD2 was identified as ubiquitin. The fragment DD3 shares significant homology to the signalling molecule 14-3-3. The partial DD4 product is homologous to the enhancer of rudimentary from Drosophila and an uncharacterized homologue of it from Arabidopsis. The fragment DD5 is similar to glucose-6-phosphate dehydrogenase which provides NADPH to the cell. The PCR-product DD6 seems to be a new leucine-rich-repeat disease resistance gene from soybean, which is significantly induced during the HR. All of the identified genes are clearly induced during a HR in infected plants of the same cultivar, indicating that results from the cell culture model system can be transferred to intact plants. These studies show that complex mRNA differential display is a powerful tool to identify new induced gene in plant-pathogen interactions. |
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Palavras-Chave: |
Soybean resistance response. |
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Thesaurus Nal: |
salicylic acid. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01935naa a2200157 a 4500
001 1869609
005 2013-08-26
008 1998 bl --- 0-- u #d
100 1 $aSEEHAUS, K.
245 $aCloning of genes by mRNA differential display induced during the hypersensitive reaction of soybean after inoculation with Pseudomonas syringae pv. glycinea.
260 $c1998
520 $aSoybean (Glycine max [L.] Merr.) cell suspension cultures (cv. Williams 82) inoculated with the pathogenic bacteria Pseudomonas syringae pv. glycinea respond with a hypersensitive reaction (HR) when the bacteria express the avirulence gene avrA. A mRNA differential display was established for this system to allow the identification of genes induced during the HR. Six PCR-fragments (DD1–DD6) from the differential display analysis were identified, which are induced during the HR. Database searches revealed that the fragment DD1 encodes chalcone isomerase and DD2 was identified as ubiquitin. The fragment DD3 shares significant homology to the signalling molecule 14-3-3. The partial DD4 product is homologous to the enhancer of rudimentary from Drosophila and an uncharacterized homologue of it from Arabidopsis. The fragment DD5 is similar to glucose-6-phosphate dehydrogenase which provides NADPH to the cell. The PCR-product DD6 seems to be a new leucine-rich-repeat disease resistance gene from soybean, which is significantly induced during the HR. All of the identified genes are clearly induced during a HR in infected plants of the same cultivar, indicating that results from the cell culture model system can be transferred to intact plants. These studies show that complex mRNA differential display is a powerful tool to identify new induced gene in plant-pathogen interactions.
650 $asalicylic acid
653 $aSoybean resistance response
700 1 $aTENHAKEN, R.
773 $tPlant Molecular Biology$gv. 38, n. 6, p. 1225-1234, 1998.
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Embrapa Trigo (CNPT) |
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| 5. | | PASQUALOTO, C.; MANRICH, A.; MARTINS, M. A. Caracterização da pele de amendoim. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 8., 2016, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2016. p. 55. Editores técnicos: Wilson Tadeu Lopes da Silva, José Manoel Marconcini, Maria Alice Martins, Lucimara Aparecida Forato, Paulino Ribeiro Villas Boas. (Embrapa Instrumentação. Documentos, 61).| Tipo: Artigo em Anais de Congresso |
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| 13. | | CASTRO, A. C. R. de; LOPES, V. Monstera adansonii: Costela-de-adão. In: CORADIN, L.; CAMILLO, J.; PAREYN, F. G. C. (Ed.). Espécies nativas da flora brasileira de valor econômico atual ou potencial: plantas para o futuro: região Nordeste. Brasília, DF: MMA, 2018. cap. 5 p. 1183-1186| Tipo: Capítulo em Livro Técnico-Científico |
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