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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/04/2007 |
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Data da última atualização: |
08/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JUST, B. J.; SANTOS, C. A. F.; FONSECA, M. E. N.; BOITEUX, L. S.; OLOIZA, B. B.; SIMON, P. W. |
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Afiliação: |
CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
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Título: |
Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, Berlin, v. 114, n. 4, p. 693-704, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Carotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors IX- and /3- carotene. Carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. In this study, we utilized published carrot sequences as well as heterologous PCR approaches with primers derived from sequence information of other plant species to isolate 24 putative genes coding for carotenoid bio-synthesis enzymes in carrot. Twenty-two of these genes were placed on the carrot genetic linkage map developed from a cross between orange-rooted and white- rooted carrot. The carotenoid genes were distributed in eight of the nine linkage groups in the carrot genome recommending their use for merging maps. Two genes co-localized with a genomic region spanning one of the most significant quantitative trait loci (QTL) for carotenoid accumulation. Carotenoid biosynthesis cDNAs linked to root color mutations and to QTL for carotenoid accumulation may suggest a functional role for them as candidate genes. RACE PCR and reverse transcriptase PCR were used to amplify the full-length transcript for twenty expressed carotenoid biosynthesis genes and sequences were submitted to GenBank. The cloning and sequence information of these' genes is useful for PCR-based expression studies and may point toward transgenic approaches to manipulate carotenoid content in carrot. MenosCarotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors IX- and /3- carotene. Carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. In this study, we utilized published carrot sequences as well as heterologous PCR approaches with primers derived from sequence information of other plant species to isolate 24 putative genes coding for carotenoid bio-synthesis enzymes in carrot. Twenty-two of these genes were placed on the carrot genetic linkage map developed from a cross between orange-rooted and white- rooted carrot. The carotenoid genes were distributed in eight of the nine linkage groups in the carrot genome recommending their use for merging maps. Two genes co-localized with a genomic region spanning one of the most significant quantitative trait loci (QTL) for carotenoid accumulation. Carotenoid biosynthesis cDNAs linked to root color mutations and to QTL for carotenoid accumulation may suggest a functional role for them as candidate genes. RACE PCR and reverse transcriptase PCR were used to amplify the full-length transcript for twenty expressed carotenoid biosynthesis genes and sequences were submitted to GenBank. The cloning and sequence information of these' genes is useful for PCR-based expression studies and may point toward transgenic approaches t... Mostrar Tudo |
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Palavras-Chave: |
Caracterização; Carrot; Mapeamento; Sequência. |
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Thesagro: |
Cenoura; Daucus Carota; Genoma. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/35108/1/OPB1009.pdf
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Marc: |
LEADER 02350naa a2200265 a 4500
001 1158533
005 2018-06-08
008 2007 bl uuuu u00u1 u #d
100 1 $aJUST, B. J.
245 $aCarotenoid biosynthesis structural genes in carrot (Daucus carota)$bisolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping.
260 $c2007
520 $aCarotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors IX- and /3- carotene. Carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. In this study, we utilized published carrot sequences as well as heterologous PCR approaches with primers derived from sequence information of other plant species to isolate 24 putative genes coding for carotenoid bio-synthesis enzymes in carrot. Twenty-two of these genes were placed on the carrot genetic linkage map developed from a cross between orange-rooted and white- rooted carrot. The carotenoid genes were distributed in eight of the nine linkage groups in the carrot genome recommending their use for merging maps. Two genes co-localized with a genomic region spanning one of the most significant quantitative trait loci (QTL) for carotenoid accumulation. Carotenoid biosynthesis cDNAs linked to root color mutations and to QTL for carotenoid accumulation may suggest a functional role for them as candidate genes. RACE PCR and reverse transcriptase PCR were used to amplify the full-length transcript for twenty expressed carotenoid biosynthesis genes and sequences were submitted to GenBank. The cloning and sequence information of these' genes is useful for PCR-based expression studies and may point toward transgenic approaches to manipulate carotenoid content in carrot.
650 $aCenoura
650 $aDaucus Carota
650 $aGenoma
653 $aCaracterização
653 $aCarrot
653 $aMapeamento
653 $aSequência
700 1 $aSANTOS, C. A. F.
700 1 $aFONSECA, M. E. N.
700 1 $aBOITEUX, L. S.
700 1 $aOLOIZA, B. B.
700 1 $aSIMON, P. W.
773 $tTheoretical and Applied Genetics, Berlin$gv. 114, n. 4, p. 693-704, 2007.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| 1. |  | JUST, B. J.; SANTOS, C. A. F.; FONSECA, M. E. N.; BOITEUX, L. S.; OLOIZA, B. B.; SIMON, P. W. Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping. Theoretical and Applied Genetics, Berlin, v. 114, n. 4, p. 693-704, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Semiárido. |
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