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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
27/02/2014 |
Data da última atualização: |
21/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, G. T.; SILVA, C. B. C.; JANK, L.; SOUZA, A. P. |
Afiliação: |
GUILHERME TOLEDO-SILVA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.; CLAUDIO BENICIO CARDOSO-SILVA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil; LIANA JANK, CNPGC; ANETE PEREIRA SOUZA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, São Paulo, BraziL. |
Título: |
De novo transcriptome assembly for the tropical grass Panicum maximum Jacq. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
PLOS ONE, v. 8, n. 7, p. 1-10, july 2013 |
Idioma: |
Inglês |
Conteúdo: |
Guinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcriptome of P. maximum using highthroughput sequencing. The biological information provided here will aid in gene expression studies and marker-assisted selection-based breeding research in tropical grasses. MenosGuinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcrip... Mostrar Tudo |
Thesagro: |
Alimento animal; Carne; Graminea tropical; Panicum maximum. |
Thesaurus Nal: |
Beef cattle; Megathyrsus maximus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/98451/1/deb-pone.0070781-1..10-fetchObject.action.pdf
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Marc: |
LEADER 02333naa a2200229 a 4500 001 1981465 005 2018-02-21 008 2013 bl uuuu u00u1 u #d 100 1 $aSILVA, G. T. 245 $aDe novo transcriptome assembly for the tropical grass Panicum maximum Jacq.$h[electronic resource] 260 $c2013 520 $aGuinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcriptome of P. maximum using highthroughput sequencing. The biological information provided here will aid in gene expression studies and marker-assisted selection-based breeding research in tropical grasses. 650 $aBeef cattle 650 $aMegathyrsus maximus 650 $aAlimento animal 650 $aCarne 650 $aGraminea tropical 650 $aPanicum maximum 700 1 $aSILVA, C. B. C. 700 1 $aJANK, L. 700 1 $aSOUZA, A. P. 773 $tPLOS ONE$gv. 8, n. 7, p. 1-10, july 2013
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
10/01/2011 |
Data da última atualização: |
02/05/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOUZA, D. dos S. DE L. E; SOUZA JUNIOR, J. D. A. DE; GROSSI DE SÁ, M.; ROCHA, T. L.; FRAGOSO, R. da R.; BARBOSA, A. E. A. DE D.; OLIVEIRA, G. R. DE; NAKASU, E. Y. T.; SOUSA, B. A. DE; PIRES, N. F.; DUSI, D. M. de A.; CARNEIRO, R. M. D. G.; PINTO, E. R. de C.; ALMEIDA-ENGLER, J. DE; ENGLER, G.; MARTINS-DE-SÁ, C.; SA, M. F. G. de. |
Afiliação: |
DJAIR DOS SANTOS DE LIMA E SOUZA, UNB; JOSÉ DIJAIR ANTONINO DE SOUZA JUNIOR, UNB; MAIRA GROSSI DE SÁ; THALES LIMA ROCHA, CENARGEN; RODRIGO DA ROCHA FRAGOSO, CPAC; AULUS ESTEVÃO ANJOS DE DEUS BARBOSA, UCB; GUSTAVO RAMOS DE OLIVEIRA; ERICH YUKIO TEMPEL NAKASU; BRUNA DE ARAUJO SOUSA, UCB; NATÁLIA FAUSTINO PIRES; DIVA MARIA DE ALENCAR DUSI, CENARGEN; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; EDUARDO ROMANO DE CAMPOS PINTO, CENARGEN; JANICE DE ALMEIDA ENGLER, INRA; GILBERT ENGLER, INRA; CESAR MARTINS DE SÁ, UNB; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Plant Science, v. 180, p. 276-282, 2011. |
DOI: |
10.1016/j.plantsci.2010.09.003 |
Idioma: |
Inglês |
Thesagro: |
Fumo; Nematóide. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202170/1/1-s2.0-S0168945210002591-main.pdf
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Marc: |
LEADER 01061naa a2200337 a 4500 001 1872576 005 2024-05-02 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1016/j.plantsci.2010.09.003$2DOI 100 1 $aSOUZA, D. dos S. DE L. E 245 $aEctopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site.$h[electronic resource] 260 $c2011 650 $aFumo 650 $aNematóide 700 1 $aSOUZA JUNIOR, J. D. A. DE 700 1 $aGROSSI DE SÁ, M. 700 1 $aROCHA, T. L. 700 1 $aFRAGOSO, R. da R. 700 1 $aBARBOSA, A. E. A. DE D. 700 1 $aOLIVEIRA, G. R. DE 700 1 $aNAKASU, E. Y. T. 700 1 $aSOUSA, B. A. DE 700 1 $aPIRES, N. F. 700 1 $aDUSI, D. M. de A. 700 1 $aCARNEIRO, R. M. D. G. 700 1 $aPINTO, E. R. de C. 700 1 $aALMEIDA-ENGLER, J. DE 700 1 $aENGLER, G. 700 1 $aMARTINS-DE-SÁ, C. 700 1 $aSA, M. F. G. de 773 $tPlant Science$gv. 180, p. 276-282, 2011.
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