Data/hora: 10/06/2024 06:22:18

Biblioteca(s): 	Embrapa Agrobiologia; Embrapa Mandioca e Fruticultura.	
Data corrente: 	28/02/1992	
Data da última atualização: 	27/06/1995	
Autoria: 	BONFANTE FASOLO, P.; VIAN, B.; PEROTTO, S.; FACCIO, A.; KNOX, J. P.	
Título: 	Cellulose and pectin localization in roots of mycorrhizal Allium porrum: labelling continuity between host cell wall and interfacial material.	
Ano de publicação: 	1990	
Fonte/Imprenta: 	Planta, v.180, p.537-547, 1990.	
Idioma: 	Inglês	
Conteúdo: 	Two different types of contacts (or interfaces) exist between the plant host and the fungus during the vesicular-arbuscular mycorrhizal symbiosis, depending on whether the fungus is intercellular or intracellular. In the first case, the walls of the partners are in contact, while in the second case the fungal wall is separated from the host cytoplasm by the invaginated host plasmamembrana and by an interfacial material. In order to verify the origin of the interfacial material, affinity techniques which allow identification in situ of cell-wall components, were used. Cellobiohydrolase (CBH I) that binds to cellulose and a monoclonal antibody (JIM 5) that reacts with pectic components were tested on roots of Allium porrum L. (leek) colonized by Glomus versiforme (Karst.) Berch. Both probes gave a labelling specific for the host cell wall, but each probe labelled over specific and distinct areas. The CBH I -colloidal gold complex heavily labelled the thick epidermal cell walls, whereas JIM 5 only labelled this area weakly. Labelling of the hypodermis was mostly on intercellular material after treatment with JIM 5 and only on the wall when CBH I was used. Suberin bands found on the radial walls were never labelled. These components show a similar distribution in the interfacial material laid down around tehe vesicular-arbuscular mycorrhizal fungus indicating that the interfacial material is of host origin.	
Palavras-Chave: 	Allium - Cellulose - Cell Wall - Mycorrhiza (vesicular-arbuscular) - Pectin; Cell wall; Mycorrhiza; Pectin.	
Thesagro: 	Allium Porrum; Método; Micorriza; Microscopia Eletrônica; Polissacarídeo.	
Thesaurus Nal: 	Allium; cellulose.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (16332-1) - ADC - SP - -- -  -  - 14401 - DIS
CNPMF - (15150-1) - ADC - SP - -- - S4651 -  - S4651 - DIS
CNPMF - (15548-1) - ADC - SP - -- - S4651 -  - S4651 - DIS