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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
02/12/2002 |
Data da última atualização: |
02/12/2002 |
Autoria: |
WINKELMAN, G. L.; ROBERTS, R. M.; PETERSON, A. J.; ALEXENKO, A. P.; EALY, A. D. |
Título: |
Identification of the expressed forms of ovine interferon-tau in the periimplantation conceptus: sequence relationships and comparative biological activities. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Biology of Reproduction, v. 61, n. 6, p. 1592-1600, 1999. |
Idioma: |
Inglês |
Conteúdo: |
Interferon-tau (IFN-T) is secreted from trophectoderm of periimplantation ruminant conceptuses and is a critical component of pregnancy recognition. Multiple genes encode IFN-T. The objectives of this study were to identify expressed forms of ovine IFN-T and to compare their biological activities. Sequences analyzed after cloning 36 reverse transcription-polymerase chain reaction products of ovine conceptus RNA provided seven new cDNA that were similar in sequence to previously cloned forms (p3, p6, and p8 cDNA). Phylogenetic analysis of amino acid sequence for all new and previously reported forms showed that ovine IFN-T forms can be divided into three main groups. Equivalent amounts of mRNA for p3, p6, and p8 forms were detected in conceptuses following RNase protection. Recombinant p3 and p8 protein had similar antiviral activity on ovine and bovine cells whereas p6 protein was less active. The p3 form was the most potent of the three in its ability to extend estrous cycle length in nonpregnant ewes. In summary, there appeared to be three main groups of ovine IFN-T, each containing several variant forms. Antiviral activity was not particularly well correlated with ability to prevent luteolysis, suggesting that distinct intracellular mechanisms are used to exert the various actions of IFN-T.
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Palavras-Chave: |
IFN; Interferon. |
Thesagro: |
Endocrinologia; Hormônio; Ovino; Reprodução Animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02041naa a2200241 a 4500 001 1528922 005 2002-12-02 008 1999 bl uuuu u00u1 u #d 100 1 $aWINKELMAN, G. L. 245 $aIdentification of the expressed forms of ovine interferon-tau in the periimplantation conceptus$bsequence relationships and comparative biological activities. 260 $c1999 520 $aInterferon-tau (IFN-T) is secreted from trophectoderm of periimplantation ruminant conceptuses and is a critical component of pregnancy recognition. Multiple genes encode IFN-T. The objectives of this study were to identify expressed forms of ovine IFN-T and to compare their biological activities. Sequences analyzed after cloning 36 reverse transcription-polymerase chain reaction products of ovine conceptus RNA provided seven new cDNA that were similar in sequence to previously cloned forms (p3, p6, and p8 cDNA). Phylogenetic analysis of amino acid sequence for all new and previously reported forms showed that ovine IFN-T forms can be divided into three main groups. Equivalent amounts of mRNA for p3, p6, and p8 forms were detected in conceptuses following RNase protection. Recombinant p3 and p8 protein had similar antiviral activity on ovine and bovine cells whereas p6 protein was less active. The p3 form was the most potent of the three in its ability to extend estrous cycle length in nonpregnant ewes. In summary, there appeared to be three main groups of ovine IFN-T, each containing several variant forms. Antiviral activity was not particularly well correlated with ability to prevent luteolysis, suggesting that distinct intracellular mechanisms are used to exert the various actions of IFN-T. 650 $aEndocrinologia 650 $aHormônio 650 $aOvino 650 $aReprodução Animal 653 $aIFN 653 $aInterferon 700 1 $aROBERTS, R. M. 700 1 $aPETERSON, A. J. 700 1 $aALEXENKO, A. P. 700 1 $aEALY, A. D. 773 $tBiology of Reproduction$gv. 61, n. 6, p. 1592-1600, 1999.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
16/02/2016 |
Data da última atualização: |
18/02/2016 |
Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
Autoria: |
LEPSCH, I. F.; ESPINDOLA, C. R.; VISCHI FILHO, O. J.; HERNANI, L. C.; SIQUEIRA, D. S. (ed.). |
Afiliação: |
IGO FERNANDO LEPSCH, IAC; CARLOS ROBERTO ESPINDOLA, UNICAMP; OSWALDO JULIO VISCHI FILHO, SEC. AGR. ABAST. EST. SP; LUIS CARLOS HERNANI, CNPS; DIEGO SILVA SIQUEIRA, UNESP. |
Título: |
Manual para levantamento utilitário e classificação de terras no sistema de capacidade de uso. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2015. |
Páginas: |
170 p. |
Descrição Física: |
il. color. |
Idioma: |
Português |
Conteúdo: |
Levantamento utilitário de terras. Classes de capacidade de uso das terras. |
Palavras-Chave: |
Ciência do solo; Levantamento do solo. |
Thesagro: |
Pedologia; Reconhecimento do solo. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 00737nam a2200217 a 4500 001 2037065 005 2016-02-18 008 2015 bl uuuu 00u1 u #d 100 1 $aLEPSCH, I. F. 245 $aManual para levantamento utilitário e classificação de terras no sistema de capacidade de uso. 260 $aViçosa, MG: Sociedade Brasileira de Ciência do Solo$c2015 300 $a170 p.$cil. color. 520 $aLevantamento utilitário de terras. Classes de capacidade de uso das terras. 650 $aPedologia 650 $aReconhecimento do solo 653 $aCiência do solo 653 $aLevantamento do solo 700 1 $aESPINDOLA, C. R. 700 1 $aVISCHI FILHO, O. J. 700 1 $aHERNANI, L. C. 700 1 $aSIQUEIRA, D. S.
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