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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/10/2002 |
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Data da última atualização: |
30/10/2002 |
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Autoria: |
McEVORY, T. G.; COULL, G. D.; BROADBENT, P. J.; HUTCHINSON, J. S. M.; SPEAKE, B. K. |
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Título: |
Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 118, n. 1, p. 163-170, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Cattle, pig and sheep oocytes isolated from healthy cumulus-oocyte complexes were pooled, within species, to provide samples of immature denuded oocytes with intact zona pellucida (n = 1000 per sample) for determination of fatty acid mass and composition in total lipid, constituent phospholipid and triglyceride. Acyl-containing' lipid extracts, transmethylated in the presence of a reference penta-decaenoic acid (15:0), yielded fatty acid methyl esters which were analysed by gas chromatography. Mean (:t SEM) fatty acid content in samples of pig oocytes (161 +- 18 ug per 1000 oocytes) was greater than that in cattle (63 +- 6 ug; P < 0.01) and sheep oocytes (89 +- t 7 ug; P < 0.05). Of 24 fatty acids detected, palmitic (16:0; 25-35%, w/w), stearic (18:0; 14-16%) and oleic (18:1n-9; 22-26%) acids were most prominent in alI three species. Saturated fatty acids (mean = 45-55%, w/w) were more abundant than mono- (27-34%) or polyunsaturates (11-21%). Fatty acids of the n-6 series, notably linoleic (18:2n-6; 5-8%, w/w) and arachidonic acid (20:4n-6; 1-3%), were the most abundant polyunsaturates. Phospholipid consistently accounted for a quarter of alI fatty acids in the three species, but ruminant oocytes had a lower complement of polyunsaturates (14-19%, w/w) in this fraction than pig oocytes (34%, w/w) which, for example, had a three- to fourfold greater linoleic acid content. An estimated 74 ng of fatty acid was sequestered in the triglyceride fraction of individual pig oocytes compared with 23-25 ng in ruminant ) oocytes (P < 0.01). It is concluded that the greater fatty acid content of pig oocytes is primarily due to more abundant triglyceride reserves. Furthermore, this species- specific difference, and that in respect of polyunsaturated fatty acid reserves, mar underlie the contrasting chilling, culture and cryopreservation sensitivities of embryos derived from pig and ruminant (cattle, sheep) oocytes. MenosCattle, pig and sheep oocytes isolated from healthy cumulus-oocyte complexes were pooled, within species, to provide samples of immature denuded oocytes with intact zona pellucida (n = 1000 per sample) for determination of fatty acid mass and composition in total lipid, constituent phospholipid and triglyceride. Acyl-containing' lipid extracts, transmethylated in the presence of a reference penta-decaenoic acid (15:0), yielded fatty acid methyl esters which were analysed by gas chromatography. Mean (:t SEM) fatty acid content in samples of pig oocytes (161 +- 18 ug per 1000 oocytes) was greater than that in cattle (63 +- 6 ug; P < 0.01) and sheep oocytes (89 +- t 7 ug; P < 0.05). Of 24 fatty acids detected, palmitic (16:0; 25-35%, w/w), stearic (18:0; 14-16%) and oleic (18:1n-9; 22-26%) acids were most prominent in alI three species. Saturated fatty acids (mean = 45-55%, w/w) were more abundant than mono- (27-34%) or polyunsaturates (11-21%). Fatty acids of the n-6 series, notably linoleic (18:2n-6; 5-8%, w/w) and arachidonic acid (20:4n-6; 1-3%), were the most abundant polyunsaturates. Phospholipid consistently accounted for a quarter of alI fatty acids in the three species, but ruminant oocytes had a lower complement of polyunsaturates (14-19%, w/w) in this fraction than pig oocytes (34%, w/w) which, for example, had a three- to fourfold greater linoleic acid content. An estimated 74 ng of fatty acid was sequestered in the triglyceride fraction of individual pig oocytes co... Mostrar Tudo |
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Palavras-Chave: |
Lipídios; Oócitos; Ovocito. |
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Thesagro: |
Bovino; Ovino; Reprodução Animal; Suíno. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02644naa a2200253 a 4500
001 1528781
005 2002-10-30
008 2000 bl uuuu u00u1 u #d
100 1 $aMcEVORY, T. G.
245 $aFatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida.
260 $c2000
520 $aCattle, pig and sheep oocytes isolated from healthy cumulus-oocyte complexes were pooled, within species, to provide samples of immature denuded oocytes with intact zona pellucida (n = 1000 per sample) for determination of fatty acid mass and composition in total lipid, constituent phospholipid and triglyceride. Acyl-containing' lipid extracts, transmethylated in the presence of a reference penta-decaenoic acid (15:0), yielded fatty acid methyl esters which were analysed by gas chromatography. Mean (:t SEM) fatty acid content in samples of pig oocytes (161 +- 18 ug per 1000 oocytes) was greater than that in cattle (63 +- 6 ug; P < 0.01) and sheep oocytes (89 +- t 7 ug; P < 0.05). Of 24 fatty acids detected, palmitic (16:0; 25-35%, w/w), stearic (18:0; 14-16%) and oleic (18:1n-9; 22-26%) acids were most prominent in alI three species. Saturated fatty acids (mean = 45-55%, w/w) were more abundant than mono- (27-34%) or polyunsaturates (11-21%). Fatty acids of the n-6 series, notably linoleic (18:2n-6; 5-8%, w/w) and arachidonic acid (20:4n-6; 1-3%), were the most abundant polyunsaturates. Phospholipid consistently accounted for a quarter of alI fatty acids in the three species, but ruminant oocytes had a lower complement of polyunsaturates (14-19%, w/w) in this fraction than pig oocytes (34%, w/w) which, for example, had a three- to fourfold greater linoleic acid content. An estimated 74 ng of fatty acid was sequestered in the triglyceride fraction of individual pig oocytes compared with 23-25 ng in ruminant ) oocytes (P < 0.01). It is concluded that the greater fatty acid content of pig oocytes is primarily due to more abundant triglyceride reserves. Furthermore, this species- specific difference, and that in respect of polyunsaturated fatty acid reserves, mar underlie the contrasting chilling, culture and cryopreservation sensitivities of embryos derived from pig and ruminant (cattle, sheep) oocytes.
650 $aBovino
650 $aOvino
650 $aReprodução Animal
650 $aSuíno
653 $aLipídios
653 $aOócitos
653 $aOvocito
700 1 $aCOULL, G. D.
700 1 $aBROADBENT, P. J.
700 1 $aHUTCHINSON, J. S. M.
700 1 $aSPEAKE, B. K.
773 $tJournal of Reproduction and Fertility$gv. 118, n. 1, p. 163-170, 2000.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 16 | |
| 3. |  | SILVA, R. de O.; BARIONI, L. G.; HALL, J. A. J.; MATSUURA, M. F.; ALBERTINI, T. Z.; FERNANDES, F. A.; MORAN, D. Increasing beef production could lower greenhouse gas emissions in Brazil if decoupled from deforestation. Nature Climate Change, v. 6, n. 5, p. 493-497, May 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Meio Ambiente. |
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| 8. | | MACHADO, Y. M. S.; HEPP, S. S.; POLINO, R. do C.; BASTIANI, M. O.; LAMEGO, F. P. Manejo integrado de caruru resistente a herbicidas: combinando estratégias. In: SEMANA INTEGRADA UFPEL, 9.; CONGRESSO DE INICIAÇÃO CIENTÍFICA, 32., 2023, Pelotas. Anais [eletrônicos...]. Pelotas: UFPEL, 2023. CIC UFPEL.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 9. | | ALENCAR, A. M. da S.; OLIVEIRA, V. X. A.; SILVA, E. S. e; PEDROZO, C. A.; BATISTA, K. D. Sobrevivência e crescimento inicial de Castanheira-do-Brasil em Roraima. In: SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA NO ESTADO DE RORAIMA, 10., 2015, Boa Vista. Resumos... Boa Vista, RR: UERR, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 11. | | ANDRADE, A. R. O.; DORVA, M. E. M. C.; ANDRADE, S. M. O. de; MARQUES, A.; SILVA, B. A. K. da; ANDREOTTI, R. Phlebotomine fauna in the Ponta Porã city: epidemiological importance in border line between Brazil and Paraguay. Asian Pacific Journal of Tropical Disease, v. 2, n. 5, p. 362-366, Oct. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 15. | | NASCIMENTO, M. A.; DA SILVA, L. C.; FURTADO, R. F.; OLIVEIRA, M. A.; MENDES, L. G.; ALVES, C. R. Uso de goma de cajueiro e gelatina para a formação de microcápsulas de óleo de pequi por coacervação complexa. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25., 2016, Gramado. Anais... Gramado: sbCTA-RS, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 16. | | BERNARDI, A.C.C.; SOUZA, G. B.; MARANHÃO, G. B.; SANTOS, K. E. L.; LUCHIARI-JUNIOR, A.; BASSOI, L. H.; RABELLO, L. M.; INAMASU, R. Y.; VAZ, C. M. P. Variabilidade espacial solo avaliada pela condutividade elétrica aparente e espectroscopia de fluorescência de raio-X. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 101-104. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Instrumentação; Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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