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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/05/2024 |
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Data da última atualização: |
20/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOUVEIA JÚNIOR, J.; GARCIA. J. A. B.; LINO, R. A.; SOUZA, J. M. C.; MORAES, E. H. B. K.; SILVA, L. F. C. e; TOMICH, T. R.; BATISTA, E. D. |
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Afiliação: |
JURANDY GOUVEIA JÚNIOR, UNIVERSIDADE FEDERAL DE MATO GROSSO; JAVIER A. B. GARCIA, UNIVERSIDADE FEDERAL DE LAVRAS; RAYANE A. LINO, UNIVERSIDADE FEDERAL DE LAVRAS; JULIA M. C. SOUZA, UNIVERSIDADE FEDERAL DE LAVRAS; EDUARDO H. B. K. MORAES, UNIVERSIDADE FEDERAL DE MATO GROSSO; LUIZ F. COSTA E SILVA, ALLTECH DO BRASIL; THIERRY RIBEIRO TOMICH, CNPGL; ERICK D. BATISTA, UNIVERSIDADE FEDERAL DE MATO GROSSO. |
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Título: |
Effects of trace mineral source and exogenous enzymes on ruminal in vitro fermentation of roughage-based or concentrate-based simulated diets. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Animal Feed Science and Technology, v. 310, 115930, 2024. |
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DOI: |
https://doi.org/10.1016/j.anifeedsci.2024.115930 |
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Idioma: |
Inglês |
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Conteúdo: |
Two experiments evaluated the effect of trace mineral (TM) source (inorganic [ITM] and organic [OTM]) and 4 exogenous enzymes treatments [(1) control, without exogenous enzymes; (2) amylolytic enzyme [AMY; 1 g of Amaize®/kg of substrate dry matter (DM)]; (3) fibrolytic enzyme (FIB; 2 g of Fibrozyme®/kg of substrate DM; and (4) a multi-enzyme preparation (SSF; 2 g of Allzyme® SSF/kg of substrate DM] on in vitro fermentation parameters of roughage-based or concentrate-based diets (Exp. 1 and 2, respectively). Two roughages were used in each experiment [high- and low-quality tropical grass hay in Exp. 1 (HQH and LQH, respectively), and corn silage and sugarcane bagasse in Exp. 2 (CS and SB, respectively)]. Each experiment was conducted as a randomized complete block (n = 3). Mixed ruminal microorganisms were incubated in anaerobic media containing 300 mg of substrate diet and 50 mL rumen fluid:buffer. Incubations were performed in batch cultures for 48 h (Exp. 1) or 24 h (Exp. 2) at 39◦C. There were no three-way interactions observed for Exp. 1 or 2. Roughage × TM source interactions (P < 0.02) were verified for total gas production (GP), partioning factor (PF), methane (CH4) production and yield in both experiments. In Exp. 1, total GP of HQH and CS decreased about 18% with OTM (P < 0.02), while for LQH and SB they increased in the same proportion. The PF of HQH and CS were increased (P < 0.01), while LQH and SB decreased by 12% (P < 0.05) when OTM was added. The CH4 yield (mL/g DMd) decreased when LQH and SB were incubated with ITM (P < 0.02). The HQH have lower pH compared with the LQH (P < 0.001; 6.56 vs. 6.72). Additionally, PF presented TM source × enzyme interaction (P = 0.05). When ITM was included, PF was higher with addition of AMY than SSF (P = 0.04), but they were similar to CON and FIB. When OTM was supplemented, PF was greater with SSF compared with FIB (P = 0.04), but presented similar values to CON and AMY. In Exp. 2, GP was lower when SB and ITM was incubated together (P = 0.02; 141 vs. 175 mL/g DM), while for CS there was no difference between ITM sources (P = 0.17). The CH4 yield reduced when OTM was combined with CS or when ITM was added with SB (P < 0.02). The PF of CS increased in 37% (P < 0.01), while SB decreased by 23% (P < 0.05) when OTM was added. As verified in Exp. 1, roughage affected pH and CS significantly reduced pH compared with SB (P < 0.001; 6.69 vs. 6.81). In conclusion, the in vitro treatment of substrates with different roughage-to-concentrate ratios with exogenous enzymes were minorly impacted by the TM source. The incubation of low-quality roughages with ITM source increased PF and decreased GP, which led to a consistent reduction in the CH4 yields. Additionally, IVDMD increased and lowered greenhouse gas production by OTM inclusion in high-concentrate substrate with CS, resulting in a favorable impact on rumen fermentation efficiency. MenosTwo experiments evaluated the effect of trace mineral (TM) source (inorganic [ITM] and organic [OTM]) and 4 exogenous enzymes treatments [(1) control, without exogenous enzymes; (2) amylolytic enzyme [AMY; 1 g of Amaize®/kg of substrate dry matter (DM)]; (3) fibrolytic enzyme (FIB; 2 g of Fibrozyme®/kg of substrate DM; and (4) a multi-enzyme preparation (SSF; 2 g of Allzyme® SSF/kg of substrate DM] on in vitro fermentation parameters of roughage-based or concentrate-based diets (Exp. 1 and 2, respectively). Two roughages were used in each experiment [high- and low-quality tropical grass hay in Exp. 1 (HQH and LQH, respectively), and corn silage and sugarcane bagasse in Exp. 2 (CS and SB, respectively)]. Each experiment was conducted as a randomized complete block (n = 3). Mixed ruminal microorganisms were incubated in anaerobic media containing 300 mg of substrate diet and 50 mL rumen fluid:buffer. Incubations were performed in batch cultures for 48 h (Exp. 1) or 24 h (Exp. 2) at 39◦C. There were no three-way interactions observed for Exp. 1 or 2. Roughage × TM source interactions (P < 0.02) were verified for total gas production (GP), partioning factor (PF), methane (CH4) production and yield in both experiments. In Exp. 1, total GP of HQH and CS decreased about 18% with OTM (P < 0.02), while for LQH and SB they increased in the same proportion. The PF of HQH and CS were increased (P < 0.01), while LQH and SB decreased by 12% (P < 0.05) when OTM was added. The CH4 yield (mL... Mostrar Tudo |
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Palavras-Chave: |
Fibrolítico; Fibrolytic. |
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Thesagro: |
Amilase; Bovino; Fermentação; Metano; Protease; Ruminante. |
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Thesaurus Nal: |
Amylases; Fermentation; Methane; Ruminants. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1164319/1/Effects-of-trace-mineral-source-and-exogenous-enzymes-on-ruminal.pdf
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Marc: |
LEADER 04009naa a2200361 a 4500
001 2164319
005 2024-05-20
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anifeedsci.2024.115930$2DOI
100 1 $aGOUVEIA JÚNIOR, J.
245 $aEffects of trace mineral source and exogenous enzymes on ruminal in vitro fermentation of roughage-based or concentrate-based simulated diets.$h[electronic resource]
260 $c2024
520 $aTwo experiments evaluated the effect of trace mineral (TM) source (inorganic [ITM] and organic [OTM]) and 4 exogenous enzymes treatments [(1) control, without exogenous enzymes; (2) amylolytic enzyme [AMY; 1 g of Amaize®/kg of substrate dry matter (DM)]; (3) fibrolytic enzyme (FIB; 2 g of Fibrozyme®/kg of substrate DM; and (4) a multi-enzyme preparation (SSF; 2 g of Allzyme® SSF/kg of substrate DM] on in vitro fermentation parameters of roughage-based or concentrate-based diets (Exp. 1 and 2, respectively). Two roughages were used in each experiment [high- and low-quality tropical grass hay in Exp. 1 (HQH and LQH, respectively), and corn silage and sugarcane bagasse in Exp. 2 (CS and SB, respectively)]. Each experiment was conducted as a randomized complete block (n = 3). Mixed ruminal microorganisms were incubated in anaerobic media containing 300 mg of substrate diet and 50 mL rumen fluid:buffer. Incubations were performed in batch cultures for 48 h (Exp. 1) or 24 h (Exp. 2) at 39◦C. There were no three-way interactions observed for Exp. 1 or 2. Roughage × TM source interactions (P < 0.02) were verified for total gas production (GP), partioning factor (PF), methane (CH4) production and yield in both experiments. In Exp. 1, total GP of HQH and CS decreased about 18% with OTM (P < 0.02), while for LQH and SB they increased in the same proportion. The PF of HQH and CS were increased (P < 0.01), while LQH and SB decreased by 12% (P < 0.05) when OTM was added. The CH4 yield (mL/g DMd) decreased when LQH and SB were incubated with ITM (P < 0.02). The HQH have lower pH compared with the LQH (P < 0.001; 6.56 vs. 6.72). Additionally, PF presented TM source × enzyme interaction (P = 0.05). When ITM was included, PF was higher with addition of AMY than SSF (P = 0.04), but they were similar to CON and FIB. When OTM was supplemented, PF was greater with SSF compared with FIB (P = 0.04), but presented similar values to CON and AMY. In Exp. 2, GP was lower when SB and ITM was incubated together (P = 0.02; 141 vs. 175 mL/g DM), while for CS there was no difference between ITM sources (P = 0.17). The CH4 yield reduced when OTM was combined with CS or when ITM was added with SB (P < 0.02). The PF of CS increased in 37% (P < 0.01), while SB decreased by 23% (P < 0.05) when OTM was added. As verified in Exp. 1, roughage affected pH and CS significantly reduced pH compared with SB (P < 0.001; 6.69 vs. 6.81). In conclusion, the in vitro treatment of substrates with different roughage-to-concentrate ratios with exogenous enzymes were minorly impacted by the TM source. The incubation of low-quality roughages with ITM source increased PF and decreased GP, which led to a consistent reduction in the CH4 yields. Additionally, IVDMD increased and lowered greenhouse gas production by OTM inclusion in high-concentrate substrate with CS, resulting in a favorable impact on rumen fermentation efficiency.
650 $aAmylases
650 $aFermentation
650 $aMethane
650 $aRuminants
650 $aAmilase
650 $aBovino
650 $aFermentação
650 $aMetano
650 $aProtease
650 $aRuminante
653 $aFibrolítico
653 $aFibrolytic
700 1 $aGARCIA. J. A. B.
700 1 $aLINO, R. A.
700 1 $aSOUZA, J. M. C.
700 1 $aMORAES, E. H. B. K.
700 1 $aSILVA, L. F. C. e
700 1 $aTOMICH, T. R.
700 1 $aBATISTA, E. D.
773 $tAnimal Feed Science and Technology$gv. 310, 115930, 2024.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | GOUVEIA JÚNIOR, J.; GARCIA. J. A. B.; LINO, R. A.; SOUZA, J. M. C.; MORAES, E. H. B. K.; SILVA, L. F. C. e; TOMICH, T. R.; BATISTA, E. D. Effects of trace mineral source and exogenous enzymes on ruminal in vitro fermentation of roughage-based or concentrate-based simulated diets. Animal Feed Science and Technology, v. 310, 115930, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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