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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/12/2023 |
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Data da última atualização: |
29/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VENKATARAMAN, M.; YÑIGEZ-GUTIERREZ, A.; INFANTE, V.; MACINTYRE, A.; FERNANDES JUNIOR, P. I.; ANÉ, J.-M.; PFLEGER, B. |
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Afiliação: |
MAYA VENKATARAMAN; AUDREY YÑIGEZ-GUTIERREZ; VALENTINA INFANTE; APRIL MACINTYRE; PAULO IVAN FERNANDES JUNIOR, CPATSA; JEAN-MICHEL ANÉ; BRIAN PFLEGER. |
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Título: |
Synthetic biology toolbox for nitrogen-fixing soil microbes. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
ACS Synthetic Biology, v. 12, n. 12, p. 3623-3634, 2023. |
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DOI: |
https://doi.org/10.1021/acssynbio.3c00414 |
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Idioma: |
Inglês |
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Conteúdo: |
The soil environment adjacent to plant roots, termed the rhizosphere, is home to a wide variety of microorganisms that can significantly affect the physiology of nearby plants. Microbes in the rhizosphere can provide nutrients, secrete signaling compounds, and inhibit pathogens. These processes could be manipulated with synthetic biology to enhance the agricultural performance of crops grown for food, energy, or environmental remediation, if methods can be implemented in these nonmodel microbes. A common first step for domesticating nonmodel organisms is the development of a set of genetic engineering tools, termed a synthetic biology toolbox. A toolbox comprises transformation protocols, replicating vectors, genome engineering (e.g., CRISPR/Cas9), constitutive and inducible promoter systems, and other gene expression control elements. This work validated synthetic biology toolboxes in three nitrogen-fixing soil bacteria: Azotobacter vinelandii, Stutzerimonas stutzeri (Pseudomonas stutzeri), and a new isolate of Klebsiella variicola. All three organisms were amenable to transformation and reporter protein expression, with several functional inducible systems available for each organism. S. stutzeri and K. variicola showed more reliable plasmid-based expression, resulting in successful Cas9 recombineering to create scarless deletions and insertions. Using these tools, we generated mutants with inducible nitrogenase activity and introduced heterologous genes to produce resorcinol products with relevant biological activity in the rhizosphere. MenosThe soil environment adjacent to plant roots, termed the rhizosphere, is home to a wide variety of microorganisms that can significantly affect the physiology of nearby plants. Microbes in the rhizosphere can provide nutrients, secrete signaling compounds, and inhibit pathogens. These processes could be manipulated with synthetic biology to enhance the agricultural performance of crops grown for food, energy, or environmental remediation, if methods can be implemented in these nonmodel microbes. A common first step for domesticating nonmodel organisms is the development of a set of genetic engineering tools, termed a synthetic biology toolbox. A toolbox comprises transformation protocols, replicating vectors, genome engineering (e.g., CRISPR/Cas9), constitutive and inducible promoter systems, and other gene expression control elements. This work validated synthetic biology toolboxes in three nitrogen-fixing soil bacteria: Azotobacter vinelandii, Stutzerimonas stutzeri (Pseudomonas stutzeri), and a new isolate of Klebsiella variicola. All three organisms were amenable to transformation and reporter protein expression, with several functional inducible systems available for each organism. S. stutzeri and K. variicola showed more reliable plasmid-based expression, resulting in successful Cas9 recombineering to create scarless deletions and insertions. Using these tools, we generated mutants with inducible nitrogenase activity and introduced heterologous genes to produce resorci... Mostrar Tudo |
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Palavras-Chave: |
Biologia sintética; Caixa de ferramentas; Cas9; CRISPR; Edição de genoma. |
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Thesagro: |
Nitrogenase; Solo. |
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Thesaurus Nal: |
Genome; Synthetic biology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159849/1/SyntheticBiologyToolboxfor-Nitrogen-FixingSoil-Microbes-2023.pdf
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Marc: |
LEADER 02445naa a2200313 a 4500
001 2159849
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024 7 $ahttps://doi.org/10.1021/acssynbio.3c00414$2DOI
100 1 $aVENKATARAMAN, M.
245 $aSynthetic biology toolbox for nitrogen-fixing soil microbes.$h[electronic resource]
260 $c2023
520 $aThe soil environment adjacent to plant roots, termed the rhizosphere, is home to a wide variety of microorganisms that can significantly affect the physiology of nearby plants. Microbes in the rhizosphere can provide nutrients, secrete signaling compounds, and inhibit pathogens. These processes could be manipulated with synthetic biology to enhance the agricultural performance of crops grown for food, energy, or environmental remediation, if methods can be implemented in these nonmodel microbes. A common first step for domesticating nonmodel organisms is the development of a set of genetic engineering tools, termed a synthetic biology toolbox. A toolbox comprises transformation protocols, replicating vectors, genome engineering (e.g., CRISPR/Cas9), constitutive and inducible promoter systems, and other gene expression control elements. This work validated synthetic biology toolboxes in three nitrogen-fixing soil bacteria: Azotobacter vinelandii, Stutzerimonas stutzeri (Pseudomonas stutzeri), and a new isolate of Klebsiella variicola. All three organisms were amenable to transformation and reporter protein expression, with several functional inducible systems available for each organism. S. stutzeri and K. variicola showed more reliable plasmid-based expression, resulting in successful Cas9 recombineering to create scarless deletions and insertions. Using these tools, we generated mutants with inducible nitrogenase activity and introduced heterologous genes to produce resorcinol products with relevant biological activity in the rhizosphere.
650 $aGenome
650 $aSynthetic biology
650 $aNitrogenase
650 $aSolo
653 $aBiologia sintética
653 $aCaixa de ferramentas
653 $aCas9
653 $aCRISPR
653 $aEdição de genoma
700 1 $aYÑIGEZ-GUTIERREZ, A.
700 1 $aINFANTE, V.
700 1 $aMACINTYRE, A.
700 1 $aFERNANDES JUNIOR, P. I.
700 1 $aANÉ, J.-M.
700 1 $aPFLEGER, B.
773 $tACS Synthetic Biology$gv. 12, n. 12, p. 3623-3634, 2023.
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Embrapa Semiárido (CPATSA) |
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| 1. |  | VENKATARAMAN, M.; YÑIGEZ-GUTIERREZ, A.; INFANTE, V.; MACINTYRE, A.; FERNANDES JUNIOR, P. I.; ANÉ, J.-M.; PFLEGER, B. Synthetic biology toolbox for nitrogen-fixing soil microbes. ACS Synthetic Biology, v. 12, n. 12, p. 3623-3634, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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