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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
27/09/2023 |
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Data da última atualização: |
25/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARDOSO, S. L.; SOUZA, P. M.; RODRIGUES, K.; MOTA, I. de S.; FERREIRA FILHO, E.; FAVARO, L. C. de L.; ARAUJO, F. S.; HOMEM-DE-MELLO, M.; PESSOA, A.; SILVEIRA, D.; BAZZO, Y. M. F.; MAGALHÃES, P. O. |
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Afiliação: |
SAMUEL LEITE CARDOSO, UNIVERSITY OF BRASILIA; PAULA MONTEIRO SOUZA, UNIVERSITY OF BRASILIA; KELLY RODRIGUES, EMBRAPA AGROENERGIA; ISABELLA DE SOUZA MOTA, UNIVERSITY OF BRASILIA; EDIVALDO FERREIRA FILHO, UNIVERSITY OF BRASÍLIA; LEIA CECILIA DE LIMA FAVARO, CNPAE; FELIPE SALDANHA ARAUJO, UNIVERSITY OF BRASILIA; MAURICIO HOMEM-DE-MELLO, UNIVERSITY OF BRASILIA; ADALBERTO PESSOA, UNIVERSITY OF SÃO PAULO; DÂMARIS SILVEIRA, UNIVERSITY OF BRASILIA; YRIS MARIA FONSECA-BAZZO, UNIVERSITY OF BRASILIA; PÉROLA OLIVEIRA MAGALHÃES, UNIVERSITY OF BRASILIA. |
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Título: |
L-Asparaginase type II from Fusarium proliferatum: heterologous expression and in silico analysis. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Pharmaceutics, v. 15, 2352, 2023. |
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DOI: |
https://doi.org/10.3390/pharmaceutics15092352 |
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Idioma: |
Inglês |
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Conteúdo: |
The search for new drug-producing microorganisms is one of the most promising situations in current world scientific scenarios. The use of molecular biology as well as the cloning of protein and compound genes is already well established as the gold standard method of increasing productivity. Aiming at this increase in productivity, this work aims at the cloning, purification and in silico analysis of L-asparaginase from Fusarium proliferatum in Komagataella phaffii (Pichia pastoris) protein expression systems. The L-asparaginase gene (NCBI OQ439985) has been cloned into Pichia pastoris strains. Enzyme production was analyzed via the quantification of aspartic B-hydroxamate, followed by purification on a DEAE FF ion exchange column. The in silico analysis was proposed based on the combined use of various technological tools. The enzymatic activity found intracellularly was 2.84 IU/g. A purification factor of 1.18 was observed. The in silico analysis revealed the position of five important amino acid residues for enzymatic activity, and likewise, it was possible to predict a monomeric structure with a C-score of 1.59. The production of the enzyme L-asparaginase from F. proliferatum in P. pastoris was demonstrated in this work, being of great importance for the analysis of new methodologies in search of the production of important drugs in therapy. |
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Palavras-Chave: |
In silico analysis. |
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Thesaurus Nal: |
Asparaginase; Fusarium proliferatum; Heterologous gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156949/1/L-Asparaginase-Type-II.pdf
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Marc: |
LEADER 02309naa a2200313 a 4500
001 2156949
005 2023-10-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/pharmaceutics15092352$2DOI
100 1 $aCARDOSO, S. L.
245 $aL-Asparaginase type II from Fusarium proliferatum$bheterologous expression and in silico analysis.$h[electronic resource]
260 $c2023
520 $aThe search for new drug-producing microorganisms is one of the most promising situations in current world scientific scenarios. The use of molecular biology as well as the cloning of protein and compound genes is already well established as the gold standard method of increasing productivity. Aiming at this increase in productivity, this work aims at the cloning, purification and in silico analysis of L-asparaginase from Fusarium proliferatum in Komagataella phaffii (Pichia pastoris) protein expression systems. The L-asparaginase gene (NCBI OQ439985) has been cloned into Pichia pastoris strains. Enzyme production was analyzed via the quantification of aspartic B-hydroxamate, followed by purification on a DEAE FF ion exchange column. The in silico analysis was proposed based on the combined use of various technological tools. The enzymatic activity found intracellularly was 2.84 IU/g. A purification factor of 1.18 was observed. The in silico analysis revealed the position of five important amino acid residues for enzymatic activity, and likewise, it was possible to predict a monomeric structure with a C-score of 1.59. The production of the enzyme L-asparaginase from F. proliferatum in P. pastoris was demonstrated in this work, being of great importance for the analysis of new methodologies in search of the production of important drugs in therapy.
650 $aAsparaginase
650 $aFusarium proliferatum
650 $aHeterologous gene expression
653 $aIn silico analysis
700 1 $aSOUZA, P. M.
700 1 $aRODRIGUES, K.
700 1 $aMOTA, I. de S.
700 1 $aFERREIRA FILHO, E.
700 1 $aFAVARO, L. C. de L.
700 1 $aARAUJO, F. S.
700 1 $aHOMEM-DE-MELLO, M.
700 1 $aPESSOA, A.
700 1 $aSILVEIRA, D.
700 1 $aBAZZO, Y. M. F.
700 1 $aMAGALHÃES, P. O.
773 $tPharmaceutics$gv. 15, 2352, 2023.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Origem |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/11/2018 |
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Data da última atualização: |
06/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CARDOSO, C. J. T.; LIMA, A. C. B. de; NOGUEIRA, E.; OLIVEIRA, M. V. M. de; MELO-STERZA, F. de A. |
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Afiliação: |
CHRISTOPHER JUNIOR TAVARES CARDOSO, UEMS; ANA CAROLINE BINE DE LIMA, UEMS; ERIKLIS NOGUEIRA, CPAP; MARCUS VINICIUS MORAIS DE OLIVEIRA, UEMS; FABIANA DE ANDRADE MELO-STERZA, UEMSD. |
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Título: |
Performance and digestive characteristics in dairy cattle maintained under grazing regime and supplemented with linseed. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 39, n. 4, p. 1805-1818, jul./ago. 2018. |
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DOI: |
10.5433/1679-0359.2018v39n4p1805 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Desempenho e características digestivas em bovinos leiteiros mantidos em regime de pastoreio suplementados com linhaça. |
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Conteúdo: |
Linseed is characterized by the rich unsaturated fatty acids and slow release of oil in the rumen. The objective of the present study was to evaluate the intake, rumen degradability, and digestibility of dairy cattle maintained under grazing regime and supplemented with linseed. Twelve Girolando (¾ Holstein × ¼ Gir) cows with a mean age of 5.2 ± 1.9 years and body weight of 583.9 ± 67.6 kg were used. The cows were maintained in a rotational grazing system on the mombaça grass (Panicum maximum) and divided into 2 experimental groups. The CONTR (n = 6) group received only the base diet (pasture) and the LINS group (n = 6) received pasture plus linseed. Initially, a fifteen-day adaptation period was established to stabilize the desired consumption amount. After this period, the animals received 800 g of linseed for 126 d. The ruminal degradability was evaluated using 2 males with a cannula in the rumen, and they were provided the same experimental diet. The measured dry matter production of pasture did not show differences during the experimental period. The intake of crude protein and ethereal extract was higher, whereas neutral detergent fiber content was lower in the cows supplemented with linseed. However, this did not alter the weight gain and digestibility coefficients, except the digestibility coefficient of the ethereal extract. At the ruminal level, linseed showed low effective and potential degradability with negative effect on grass degradability. |
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Thesagro: |
Linhaça; Suplemento Alimentar; Vaca Leiteira. |
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Thesaurus NAL: |
Dairy cows; Food additives; Linseed. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187680/1/performance-and-digestive-caracteristics-with-linseed-semina-2018.pdf
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Marc: |
LEADER 02465naa a2200265 a 4500
001 2100201
005 2018-12-06
008 2018 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2018v39n4p1805$2DOI
100 1 $aCARDOSO, C. J. T.
245 $aPerformance and digestive characteristics in dairy cattle maintained under grazing regime and supplemented with linseed.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Desempenho e características digestivas em bovinos leiteiros mantidos em regime de pastoreio suplementados com linhaça.
520 $aLinseed is characterized by the rich unsaturated fatty acids and slow release of oil in the rumen. The objective of the present study was to evaluate the intake, rumen degradability, and digestibility of dairy cattle maintained under grazing regime and supplemented with linseed. Twelve Girolando (¾ Holstein × ¼ Gir) cows with a mean age of 5.2 ± 1.9 years and body weight of 583.9 ± 67.6 kg were used. The cows were maintained in a rotational grazing system on the mombaça grass (Panicum maximum) and divided into 2 experimental groups. The CONTR (n = 6) group received only the base diet (pasture) and the LINS group (n = 6) received pasture plus linseed. Initially, a fifteen-day adaptation period was established to stabilize the desired consumption amount. After this period, the animals received 800 g of linseed for 126 d. The ruminal degradability was evaluated using 2 males with a cannula in the rumen, and they were provided the same experimental diet. The measured dry matter production of pasture did not show differences during the experimental period. The intake of crude protein and ethereal extract was higher, whereas neutral detergent fiber content was lower in the cows supplemented with linseed. However, this did not alter the weight gain and digestibility coefficients, except the digestibility coefficient of the ethereal extract. At the ruminal level, linseed showed low effective and potential degradability with negative effect on grass degradability.
650 $aDairy cows
650 $aFood additives
650 $aLinseed
650 $aLinhaça
650 $aSuplemento Alimentar
650 $aVaca Leiteira
700 1 $aLIMA, A. C. B. de
700 1 $aNOGUEIRA, E.
700 1 $aOLIVEIRA, M. V. M. de
700 1 $aMELO-STERZA, F. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 39, n. 4, p. 1805-1818, jul./ago. 2018.
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Registro original: |
Embrapa Pantanal (CPAP) |
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