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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
05/09/2023 |
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Data da última atualização: |
29/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BACANELLI, G.; ARAUJO, F. R.; VERBISCK, N. V. |
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Afiliação: |
GISELE BACANELLI, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; FLABIO RIBEIRO DE ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC. |
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Título: |
Improved MALDI-TOF MS identification of Mycobacterium tuberculosis by use of an enhanced cell disruption protocol. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Microorganisms, v. 11, issue 7, article 1692, 2023. |
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DOI: |
https://doi.org/10.3390/ microorganisms11071692 |
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Idioma: |
Inglês |
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Notas: |
Communication. |
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Conteúdo: |
ABSTRACT - Mycobacterium tuberculosis is the microorganism that causes tuberculosis, a disease affecting millions of people worldwide. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast, reliable, and cost-effective method for microorganism identification which has been used for the identification of Mycobacterium spp. isolates. However, the mycobacteria cell wall is rich in lipids, which makes it difficult to obtain proteins for MALDI-TOF MS analysis. In this study, two cell preparation protocols were compared: the MycoEx, recommended by MALDI-TOF instrument manufacturer Bruker Daltonics, and the MycoLyser protocol described herein, which used the MagNA Lyser instrument to enhance cell disruption with ethanol. Cell disruption and protein extraction steps with the two protocols were performed using the Mycobacterium tuberculosis H37Rv strain, and the MALDI-TOF MS results were compared. The MycoLyser protocol allowed for improved Biotyper identification of M. tuberculosis since the log(score) values obtained with this protocol were mostly > 1.800 and significantly higher than that underwent MycoEx processing. The identification reliability was increased as well, considering the Bruker criteria. In view of these results, it is concluded that the MycoLyser protocol for mycobacterial cell disruption and protein extraction improves the MALDI-TOF MS method's efficacy for M. tuberculosis identification. |
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Palavras-Chave: |
MALDI-TOF MS. |
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Thesagro: |
Espectrometria; Microrganismo; Tuberculose. |
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Thesaurus Nal: |
Mass spectrometry; Microorganisms; Mycobacterium tuberculosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/255700/1/Improved-MALDI-TOF-MS-identification-2023.pdf
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Marc: |
LEADER 02272naa a2200253 a 4500
001 2156432
005 2023-11-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ microorganisms11071692$2DOI
100 1 $aBACANELLI, G.
245 $aImproved MALDI-TOF MS identification of Mycobacterium tuberculosis by use of an enhanced cell disruption protocol.$h[electronic resource]
260 $c2023
500 $aCommunication.
520 $aABSTRACT - Mycobacterium tuberculosis is the microorganism that causes tuberculosis, a disease affecting millions of people worldwide. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast, reliable, and cost-effective method for microorganism identification which has been used for the identification of Mycobacterium spp. isolates. However, the mycobacteria cell wall is rich in lipids, which makes it difficult to obtain proteins for MALDI-TOF MS analysis. In this study, two cell preparation protocols were compared: the MycoEx, recommended by MALDI-TOF instrument manufacturer Bruker Daltonics, and the MycoLyser protocol described herein, which used the MagNA Lyser instrument to enhance cell disruption with ethanol. Cell disruption and protein extraction steps with the two protocols were performed using the Mycobacterium tuberculosis H37Rv strain, and the MALDI-TOF MS results were compared. The MycoLyser protocol allowed for improved Biotyper identification of M. tuberculosis since the log(score) values obtained with this protocol were mostly > 1.800 and significantly higher than that underwent MycoEx processing. The identification reliability was increased as well, considering the Bruker criteria. In view of these results, it is concluded that the MycoLyser protocol for mycobacterial cell disruption and protein extraction improves the MALDI-TOF MS method's efficacy for M. tuberculosis identification.
650 $aMass spectrometry
650 $aMicroorganisms
650 $aMycobacterium tuberculosis
650 $aEspectrometria
650 $aMicrorganismo
650 $aTuberculose
653 $aMALDI-TOF MS
700 1 $aARAUJO, F. R.
700 1 $aVERBISCK, N. V.
773 $tMicroorganisms$gv. 11, issue 7, article 1692, 2023.
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