Data/hora: 10/06/2024 04:44:39

Biblioteca(s): 	Embrapa Mandioca e Fruticultura; Embrapa Trigo.	
Data corrente: 	21/03/2023	
Data da última atualização: 	04/04/2023	
Autoria: 	CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; PEREIRA, L. R.; BASTIANEL, M.; LAU, D.; CANALE, M. C.; HARAKAVA, R.; NOVELLI, V. M.; KITAJIMA, E. W. N.; ASTUA, J. de F.	
Afiliação: 	CAMILA CHABI-JESUS, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; PEDRO LUIS RAMOS-GONZÁLEZ, INSTITUTO BIOLÓGICO; ALINE DANIELE TASSI, INSTITUTO BIOLÓGICO; LAURA ROSSETTO PEREIRA, INSTITUTO BIOLÓGICO; MARINÊS BASTIANEL, CENTRO DE CITRICULTURA SYLVIO MOREIRA; DOUGLAS LAU, CNPT; MARIA CRISTINA CANALE, EMPRESA DE PESQUISA AGROPECUÁRIA E EXTENSÃO RURAL DE SANTA CATARINA; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; VALDENICE MOREIRA NOVELLI, CENTRO DE CITRICULTURA SYLVIO MOREIRA; ELLIOT WATA NABE KITAJIMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JULIANA DE FREITAS ASTUA, CNPMF.	
Título: 	Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Plants, v.12, n.6 p.1371. 2023.	
DOI: 	10.3390/plants12061371	
Idioma: 	Inglês	
Conteúdo: 	Citrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(?)RNA viruses, with ORFs in a typical organization of members of the genus Dichorhavirus, were recovered. These genomes shared 98?99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by Brevipalpus phoenicis sensu stricto. In CiBSV-infected citrus plants, B. papayensis and B. azores were found, but the virus could only be transmitted to Arabidopsis plants by B. azores. The study provides the first evidence of the role of B. azores as a viral vector and supports the assignment of CiBSV to the tentative new species Dichorhavirus australis.	
Thesagro: 	Fruta Cítrica; Vírus.	
Thesaurus Nal: 	Mononegavirales; Rhabdoviridae; Vector-borne diseases.	
Categoria do assunto: 	G Melhoramento Genético	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152548/1/plants-12-01371.pdf	
Registro original: 	Embrapa Trigo (CNPT)	
Exemplares: 	CNPMF - (33756-1) - UPC - AP - DD - Publicação digital -  -  - DIS
CNPT - (45512-1) - UPC - AP - DD -  -  -  - DIS