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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
13/01/2023 |
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Data da última atualização: |
13/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, E. R.; FREIRE, R. P.; SUZUKI, M. F.; OLIVEIRA, J. E.; YOSIDAKI, V. L.; PERONI, C. N.; SEVILHANO, T.; ZORZETO, M.; TORATI, L. S.; SOARES, C. R. J.; LIMA, I. D. de M.; KRONENBERGER, T.; MALTAROLLO, V. G.; BARTOLINI, P. |
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Afiliação: |
ELIANA ROSA LIMA, IPEN-CNEN; RENAN PASSOS FREIRE, IPEN-CNEN; MIRIAM FUSSAE SUZUKI, IPEN-CNEN; JOÃO EZEQUIEL OLIVEIRA, IPEN-CNEN; VANESSA LUNA YOSIDAKI, IPEN-CNEN; CIBELE NUNES PERONI, IPEN-CNEN; THAÍS SEVILHANO, IPEN-CNEN; MOISÉS ZORZETO, PISCICULTURA RAÇA, MT; LUCAS SIMON TORATI, CNPASA; CARLOS ROBERTO JORGE SOARES, IPEN-CNEN; IGOR DANIEL DE MIRANDA LIMA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; THALES KRONENBERGER, Eberhard Karls University Tübingen, Germany; VINICIUS GONÇALVES MALTAROLLO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; PAOLO BARTOLINI, IPEN-CNEN. |
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Título: |
Isolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biomolecules, v. 13, n. 1, art. 158, 2023. |
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ISSN: |
2218-273X |
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DOI: |
https://doi.org/10.3390/biom13010158 |
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Idioma: |
Inglês |
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Conteúdo: |
In a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies. |
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Palavras-Chave: |
Growth hormone; In silico sequencing. |
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Thesagro: |
Hormônio Animal; Peixe; Pirarucu. |
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Thesaurus Nal: |
Arapaima gigas; Hormones; Molecular models. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150987/1/biomolecules-2023.pdf
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Marc: |
LEADER 02661naa a2200397 a 4500
001 2150987
005 2023-02-13
008 2023 bl uuuu u00u1 u #d
022 $a2218-273X
024 7 $ahttps://doi.org/10.3390/biom13010158$2DOI
100 1 $aLIMA, E. R.
245 $aIsolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH).$h[electronic resource]
260 $c2023
520 $aIn a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies.
650 $aArapaima gigas
650 $aHormones
650 $aMolecular models
650 $aHormônio Animal
650 $aPeixe
650 $aPirarucu
653 $aGrowth hormone
653 $aIn silico sequencing
700 1 $aFREIRE, R. P.
700 1 $aSUZUKI, M. F.
700 1 $aOLIVEIRA, J. E.
700 1 $aYOSIDAKI, V. L.
700 1 $aPERONI, C. N.
700 1 $aSEVILHANO, T.
700 1 $aZORZETO, M.
700 1 $aTORATI, L. S.
700 1 $aSOARES, C. R. J.
700 1 $aLIMA, I. D. de M.
700 1 $aKRONENBERGER, T.
700 1 $aMALTAROLLO, V. G.
700 1 $aBARTOLINI, P.
773 $tBiomolecules$gv. 13, n. 1, art. 158, 2023.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
27/01/2021 |
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Data da última atualização: |
27/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, J. O. da C. e; BRUCKNER, C. H.; CARNEIRO, P. C. S.; RESENDE, M. D. V. de; ALVES, R. S.; SILVA, D. F. P. da. |
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Afiliação: |
JOSÉ OSMAR DA COSTA E SILVA, UFV; CLAUDIO HORST BRUCKNER, UFV; PEDRO CRESCÊNCIO SOUZA CARNEIRO, UFV; MARCOS DEON VILELA DE RESENDE, CNPCa; RODRIGO SILVA ALVES, UFV; DANIELLE FABÍOLA PEREIRA DA SILVA, UFG. |
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Título: |
Estimates of genetic parameters, genetic variability, and selection in the S1 generation of peach. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Ciência Rural, v. 50, n. 10, e20190976, 2020. |
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DOI: |
https://doi.org/10.1590/0103-8478cr20190976 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Estimativas de parâmetros genéticos, variabilidade genética e seleção em geração S 1 de pessegueiros. |
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Conteúdo: |
The growing of peach in mild winter regions is made viable through the use of genotypes that have low need for cold conditions, and this is one of the main aims of breeding for these regions. Thus, the aims of this study were to estimate genetic parameters, evaluate genetic variability, and select families adapted to mild winter regions in the S1 generation of peach through mixed model methodology (REML/BLUP). For that purpose, 22 populations, 84 families, and 2090 individuals were evaluated for the following traits: bud burst rate (BR), node density (ND), plant height (PH), and trunk diameter (TD). Genetic variability was found for all the traits. Individual heritability in the broad sense was of low and medium magnitudes. The PH trait had positive genotypic correlation of high magnitude with TD. The ND trait had moderate negative genotypic correlation with PH and TD. Clustering by the Tocher method resulted in the formation of six mutually exclusive groups. Considering selection intensity of 25%, simultaneous selection for BR, ND, and TD led to predicted gains of 11.3% for BR, 9.7% for ND, -14.2% for PH, and -14.3% for TD, showing the great potential of the germplasm evaluated. |
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Thesagro: |
Fruta; Melhoramento Vegetal; Pêssego; Prunus Persica. |
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Thesaurus NAL: |
Fruit trees; Peaches; Plant breeding. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220704/1/Estimates-of-genetic-parameters-genetic-variability.pdf
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Marc: |
LEADER 02175naa a2200289 a 4500
001 2129623
005 2021-01-27
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/0103-8478cr20190976$2DOI
100 1 $aSILVA, J. O. da C. e
245 $aEstimates of genetic parameters, genetic variability, and selection in the S1 generation of peach.$h[electronic resource]
260 $c2020
500 $aTítulo em português: Estimativas de parâmetros genéticos, variabilidade genética e seleção em geração S 1 de pessegueiros.
520 $aThe growing of peach in mild winter regions is made viable through the use of genotypes that have low need for cold conditions, and this is one of the main aims of breeding for these regions. Thus, the aims of this study were to estimate genetic parameters, evaluate genetic variability, and select families adapted to mild winter regions in the S1 generation of peach through mixed model methodology (REML/BLUP). For that purpose, 22 populations, 84 families, and 2090 individuals were evaluated for the following traits: bud burst rate (BR), node density (ND), plant height (PH), and trunk diameter (TD). Genetic variability was found for all the traits. Individual heritability in the broad sense was of low and medium magnitudes. The PH trait had positive genotypic correlation of high magnitude with TD. The ND trait had moderate negative genotypic correlation with PH and TD. Clustering by the Tocher method resulted in the formation of six mutually exclusive groups. Considering selection intensity of 25%, simultaneous selection for BR, ND, and TD led to predicted gains of 11.3% for BR, 9.7% for ND, -14.2% for PH, and -14.3% for TD, showing the great potential of the germplasm evaluated.
650 $aFruit trees
650 $aPeaches
650 $aPlant breeding
650 $aFruta
650 $aMelhoramento Vegetal
650 $aPêssego
650 $aPrunus Persica
700 1 $aBRUCKNER, C. H.
700 1 $aCARNEIRO, P. C. S.
700 1 $aRESENDE, M. D. V. de
700 1 $aALVES, R. S.
700 1 $aSILVA, D. F. P. da
773 $tCiência Rural$gv. 50, n. 10, e20190976, 2020.
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Registro original: |
Embrapa Café (CNPCa) |
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