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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/11/2022 |
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Data da última atualização: |
24/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROMERO PELÁEZ, R. D.; WISCHRAL, D.; CUNHA, J. R. B.; MENDES, T. D.; PACHECO, T. F.; SIQUEIRA, F. G. de; ALMEIDA, J. R. M. de. |
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Afiliação: |
RUBÉN DARÍO ROMERO PELÁEZ, UNIVERSIDADE DE BRASÍLIA; DAIANA WISCHRAL, CNPAE; JOICE RAÍSA BARBOSA CUNHA, CNPAE; THAIS DEMARCHI MENDES, CNPAE; THALYTA FRAGA PACHECO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Production of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Fermentation, v. 8, n. 10, 522, 2022. |
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Páginas: |
17 p. |
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ISSN: |
2311-5637 |
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DOI: |
https://doi.org/10.3390/fermentation8100522 |
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Idioma: |
Inglês |
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Conteúdo: |
This work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. MenosThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high effi... Mostrar Tudo |
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Palavras-Chave: |
Co-cultures; Ethanolic fermentation; Laccases; Panus lecomtei. |
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Thesaurus Nal: |
Cellulases; Trichoderma reesei. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148635/1/Production-of-enzymatic-extract.pdf
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Marc: |
LEADER 02486naa a2200301 a 4500
001 2148635
005 2022-11-24
008 2022 bl uuuu u00u1 u #d
022 $a2311-5637
024 7 $ahttps://doi.org/10.3390/fermentation8100522$2DOI
100 1 $aROMERO PELÁEZ, R. D.
245 $aProduction of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei.$h[electronic resource]
260 $c2022
300 $a17 p.
520 $aThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast.
650 $aCellulases
650 $aTrichoderma reesei
653 $aCo-cultures
653 $aEthanolic fermentation
653 $aLaccases
653 $aPanus lecomtei
700 1 $aWISCHRAL, D.
700 1 $aCUNHA, J. R. B.
700 1 $aMENDES, T. D.
700 1 $aPACHECO, T. F.
700 1 $aSIQUEIRA, F. G. de
700 1 $aALMEIDA, J. R. M. de
773 $tFermentation$gv. 8, n. 10, 522, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Origem |
Tipo/Formato |
Classificação |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
25/11/2022 |
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Data da última atualização: |
25/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; ESTEVES, S. N.; OLIVEIRA, H. N. DE; GIGLIOTI, R.; GIGLIOTI, C.; CARVALHO, C. DE O.; FERREZINI, J.; SCHIAVONE, D. C. |
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Afiliação: |
ANA CAROLINA DE SOUZA CHAGAS, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; HENRIQUE N. DE OLIVEIRA, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Botucatu, Botucatu, SP; RODRIGO GIGLIOTI, Faculdade de Ciências Agrárias e Veterinárias, Unesp/ Campus Jaboticabal, Jaboticabal, SP; CAROLINA GIGLIOTI, Universidade Federal de São Carlos, São Carlos, SP - Bolsistas CNPq e FAPESP; CAMILA DE O. CARVALHO, Universidade Federal de São Carlos, São Carlos, SP - Bolsistas CNPq e FAPESP; JENIFER FERREZINI, Universidade Central Paulista, São Carlos, SP - Bolsistas CNPq; DANIELE C. SCHIAVONE, Universidade Central Paulista, São Carlos, SP - Bolsistas CNPq. |
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Título: |
Parasitismo por nematóides gastrintestinais em matrizes e cordeiros criados em São Carlos, São Paulo. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinária, v. 17, Supl. 1, p. 126-132, 2008. |
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Idioma: |
Português |
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Conteúdo: |
Gastrointestinal nematode infections were evaluated in Santa Inês crossbreed sheep (Santa Inês predominance) in a rotational grazing system and in lambs kept indoors, born from the breeding of these females with purebred Santa Ines, Dorper and Suffolk males. Fecal egg counts (FEC), fecal cultures, packed cell volume (PCV) and weights were evaluated during two years. Climate data were registered. Only animals that presented FEC higher than 4,000 and/or PCV lower than 21% were drenched with anthelmintic treatment and this rational use controlled the infection in the flock satisfactorily. An adequate diet during the rainy season and supplementation in dry period were important to increase relative resistance and resilience to parasites, since Haemonchus contortus was detected year-round in this region. The peripartum physiological condition influenced gastrointestinal nematode infections significantly. Crossbred ½ Santa Inês + ½ Dorper lambs did not show a significant difference in FEC when compared with other breeds, but they had greater live weight and reached slaughter weight earlier. |
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Palavras-Chave: |
Control. |
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Thesagro: |
Haemonchus Contortus. |
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Thesaurus NAL: |
Breeding; Epidemiology; Sheep. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148750/1/ParasitismoNematoidesGastrintestinais.pdf
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Marc: |
LEADER 01943naa a2200277 a 4500
001 2148750
005 2022-11-25
008 2008 bl uuuu u00u1 u #d
100 1 $aCHAGAS, A. C. de S.
245 $aParasitismo por nematóides gastrintestinais em matrizes e cordeiros criados em São Carlos, São Paulo.$h[electronic resource]
260 $c2008
520 $aGastrointestinal nematode infections were evaluated in Santa Inês crossbreed sheep (Santa Inês predominance) in a rotational grazing system and in lambs kept indoors, born from the breeding of these females with purebred Santa Ines, Dorper and Suffolk males. Fecal egg counts (FEC), fecal cultures, packed cell volume (PCV) and weights were evaluated during two years. Climate data were registered. Only animals that presented FEC higher than 4,000 and/or PCV lower than 21% were drenched with anthelmintic treatment and this rational use controlled the infection in the flock satisfactorily. An adequate diet during the rainy season and supplementation in dry period were important to increase relative resistance and resilience to parasites, since Haemonchus contortus was detected year-round in this region. The peripartum physiological condition influenced gastrointestinal nematode infections significantly. Crossbred ½ Santa Inês + ½ Dorper lambs did not show a significant difference in FEC when compared with other breeds, but they had greater live weight and reached slaughter weight earlier.
650 $aBreeding
650 $aEpidemiology
650 $aSheep
650 $aHaemonchus Contortus
653 $aControl
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aESTEVES, S. N.
700 1 $aOLIVEIRA, H. N. DE
700 1 $aGIGLIOTI, R.
700 1 $aGIGLIOTI, C.
700 1 $aCARVALHO, C. DE O.
700 1 $aFERREZINI, J.
700 1 $aSCHIAVONE, D. C.
773 $tRevista Brasileira de Parasitologia Veterinária$gv. 17, Supl. 1, p. 126-132, 2008.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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