Registro Completo |
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
16/11/2022 |
Data da última atualização: |
16/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. K. C. da; SANTOS, M. R. A. dos. |
Afiliação: |
CAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO. |
Título: |
Induction of somatic embryos in Robusta and Conilon varieties of Coffea canephora. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
International Journal of Development Research, v. 12, n. 10, p. 59734-59737, Oct. 2022. |
ISSN: |
2230-9926 |
DOI: |
https://doi.org/10.37118/ijdr.25620.10.2022 |
Idioma: |
Inglês |
Conteúdo: |
Clonal propagation of Coffea canephora varieties is feasible through somatic embryogenesis. The aim of this study was to evaluate the induction of somatic embryos from leaves of C. canephora, Robusta and Conilon varieties, in relation to different concentrations of three growth regulators. The explants were inoculated in a MS culture medium with 2iP (isopentanyladenine), IBA (indolebutyric acid) and BA (6-benzylaminopurine), in factorial combinations, at concentrations of 1.0; 2.0; 3.0; 4.0 and 5.0 mg L-1. At 45 days of culture, the percentage of callus induction was evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The most efficient combinations of growth regulators to induce callogenesis in the explants were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 3.0 mg L-1 of IBA + 3. 0 mg L-1 of BA - for the Robusta variety; and 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. As for the average number of embryos per explant, the most efficient combinations of growth regulators were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 2.0 mg L-1 of IBA + 3 .0 mg L-1 of BA ? for the Robusta cultivar; and 4.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. |
Palavras-Chave: |
Amazônia Ocidental; Cefé Conilon; Clonal propagation; Cotyledonary embryos; Embrapa Rondônia; Embriogênese somática; Porto Velho (RO); Western Amazon. |
Thesagro: |
Café Robusta; Campo Experimental; Coffea Canephora; Cultura de Tecido. |
Thesaurus Nal: |
Demonstration farms; Somatic embryogenesis; Tissue culture. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148353/1/cpafro-18835.pdf
|
Marc: |
LEADER 02361naa a2200337 a 4500 001 2148353 005 2022-11-16 008 2022 bl uuuu u00u1 u #d 022 $a2230-9926 024 7 $ahttps://doi.org/10.37118/ijdr.25620.10.2022$2DOI 100 1 $aSILVA, C. K. C. da 245 $aInduction of somatic embryos in Robusta and Conilon varieties of Coffea canephora.$h[electronic resource] 260 $c2022 520 $aClonal propagation of Coffea canephora varieties is feasible through somatic embryogenesis. The aim of this study was to evaluate the induction of somatic embryos from leaves of C. canephora, Robusta and Conilon varieties, in relation to different concentrations of three growth regulators. The explants were inoculated in a MS culture medium with 2iP (isopentanyladenine), IBA (indolebutyric acid) and BA (6-benzylaminopurine), in factorial combinations, at concentrations of 1.0; 2.0; 3.0; 4.0 and 5.0 mg L-1. At 45 days of culture, the percentage of callus induction was evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The most efficient combinations of growth regulators to induce callogenesis in the explants were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 3.0 mg L-1 of IBA + 3. 0 mg L-1 of BA - for the Robusta variety; and 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. As for the average number of embryos per explant, the most efficient combinations of growth regulators were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 2.0 mg L-1 of IBA + 3 .0 mg L-1 of BA ? for the Robusta cultivar; and 4.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. 650 $aDemonstration farms 650 $aSomatic embryogenesis 650 $aTissue culture 650 $aCafé Robusta 650 $aCampo Experimental 650 $aCoffea Canephora 650 $aCultura de Tecido 653 $aAmazônia Ocidental 653 $aCefé Conilon 653 $aClonal propagation 653 $aCotyledonary embryos 653 $aEmbrapa Rondônia 653 $aEmbriogênese somática 653 $aPorto Velho (RO) 653 $aWestern Amazon 700 1 $aSANTOS, M. R. A. dos 773 $tInternational Journal of Development Research$gv. 12, n. 10, p. 59734-59737, Oct. 2022.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |