|
|
Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
10/08/2021 |
Data da última atualização: |
10/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ROMERO TISOT, C. M.; ZANELLA, R.; SOUZA, A. P.; LÖF, L. M.; SOARES, J. C. M.; MARQUES, M. G.; ZANELLA, E. L. |
Afiliação: |
CÂNDIDA MALDANER ROMERO TISOT, UPF; RICARDO ZANELLA, UPF; UDESC/Lages; LUCAS MALLMANN LÖF, UPF; UFSM; MARIANA GROKE MARQUES, CNPSA; ERALDO LOURENSO ZANELLA, UPF. |
Título: |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020. |
DOI: |
10.23880/oajvsr-16000202 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation. MenosAbstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addi... Mostrar Tudo |
Palavras-Chave: |
Melatonina. |
Thesagro: |
Criopreservação; Espermatozóide; Garanhão; Reprodução Animal. |
Thesaurus Nal: |
Animal reproduction; Cryopreservation; Melatonin; Reactive oxygen species; Spermatozoa; Stallions. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225036/1/final9560.pdf
|
Marc: |
LEADER 02592naa a2200337 a 4500 001 2133434 005 2021-08-10 008 2020 bl uuuu u00u1 u #d 024 7 $a10.23880/oajvsr-16000202$2DOI 100 1 $aROMERO TISOT, C. M. 245 $aMelatonin effect on cryopreserved sperm cells of crioulo stallions.$h[electronic resource] 260 $c2020 520 $aAbstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation. 650 $aAnimal reproduction 650 $aCryopreservation 650 $aMelatonin 650 $aReactive oxygen species 650 $aSpermatozoa 650 $aStallions 650 $aCriopreservação 650 $aEspermatozóide 650 $aGaranhão 650 $aReprodução Animal 653 $aMelatonina 700 1 $aZANELLA, R. 700 1 $aSOUZA, A. P. 700 1 $aLÖF, L. M. 700 1 $aSOARES, J. C. M. 700 1 $aMARQUES, M. G. 700 1 $aZANELLA, E. L 773 $tOpen Access Journal of Veterinary Science & Research$gv. 5, n. 2, 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 73 | |
6. | | SERRA, V.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LIMA, A. O. de; GASPARIN, G.; COUTINHO, L. L.; MOURÃO, G. B.; REGITANO, L. C. de A. Análise de qualidade de RNA para estudo do perfil transcriptômico de animais extremos para eficiência alimentar da raça Nelore. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 5., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação , 2013. p. 12 (Embrapa Pecuária Sudeste. Documentos, 110). Editado por Ana Rita de Araújo nogueira, simone Cristina Méo NicuraTipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
7. | | TIZIOTO, P. C.; MEIRELLES, S. L.; SOUZA, M. M. de; OLIVEIRA, P. S. N. de; SIQUEIRA, F.; ROSA, A. do N.; TULLIO, R. R. Screening for single nucleotide polymorphisms in MYOD1 gene and its association with meat tenderness in cattle. In: INTERNATIONAL CONFERENCE OF BRAZIL ASSOCIATION AND COMPUTER BIOLOGY 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianóplis: ABC3C: SolBio, 2011. ID 126Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte; Embrapa Pecuária Sudeste. |
| |
10. | | DINIZ, W. J. S.; ROSA, K. O. da; TIZIOTO, P. C.; MOURÃO, G. B.; OLIVEIRA, P. S. N. de; SOUZA, M. M. de; REGITANO, L. C. de A. FABP1 and SLC2A5 expression levels affect feed efficiency-related traits. Agri Gene, v. 15, p. 1-7, e100100, mar. 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
11. | | OLIVEIRA, P. S. N.; MALAGO JUNIOR, W.; TIZIOTO, P. C.; NASCIMENTO, M. L. do; MUDADU, M. de A.; REGITANO, L. C. de A. NEUROD1 gene is not associated with feed efficiency in Nellore cattle. In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Abstract... Guarujá:[ s.n.], 2013. AB.28.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
12. | | OLIVEIRA, P. S. N. de; TIZIOTO, P. C.; NORONHA, C. T.; MALAGO JUNIOR, W.; MUDADU, M. de A.; REGITANO, L. C. de A. A SNP in NEUROD1 is associated with production traits in Nelore beef cattle. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 51., 2014, Barra dos Coqueiros. A produção animal frente às mudanças climáticas e tecnológicas - anais. Barra dos coqueiros: Sociedade Brasileira de Zootecnia, 2014. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
13. | | SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; ZERLOTINI, A.; REGITANO, L. C. Allele specific expression analysis in bovine muscle tissue. In: INTERNATIONAL CONFERENCE OF TH AB3C, 11.; BRAZILIAN SYMPOSIUM OF BIOINFORMATICS, 2015, São Paulo. Proceedings... São Paulo: USP-São Paulo, 2015.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
14. | | ROCHA, M. I. P.; SOUZA, M. M. de; ZERLOTINI NETO, A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LIMA, A. O. de; AFONSO, J.; COUTINHO, L. L.; REGITANO, L. C. de A.; NICIURA, S. C. M. Allele-specific gene expression in liver of Nelore cattle extremes for feed efficiency. In: INTERNATIONAL SOCIETY FOR ANIMAL GENETICS CONFERENCE, 36., 2017, Dublin. Proceedings... Dublin: University College Dublin, 2017. p. 156 - 157.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
15. | | SOUZA, M. M.; ZERLOTINI, A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A. L.; MOKRY, F. B.; CESAR, A. S. M.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. A. Complex pattern of CAST allelic expression in bovine muscle tissue. In: ANNUAL INTERNATIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, 22., 2014, Boston, USA. Program... [Boston]: International Society for Computational Biology, 2014. Não paginado. ISMB 2014. Pôster E13.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
| |
16. | | OLIVEIRA, P. S. N. de; TIZIOTO, P. C.; OLIVEIRA, G. B.; CESAR, A. S. M.; DINIZ, W. J. da S.; LIMA, A. O. de; REECY, J. M.; COUTINHO, L. L.; REGITANO, L. C. de A. Differentially expressed miRNAs in skeletal muscle related to feed efficiency in Nellore cattle. Journal of Animal Science, v. 94, e-suppl. 5; Journal of Dairy Science, v. 99, e-suppl. 1, p. 159, jul. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
17. | | OLIVEIRA, P. S. N. DE; ANDRADE, B. G.; CARDOSO, T. F.; PASCHOAL, J. J.; JOSAHKIAN, L. A.; ALMEIDA, L. F.; MARCONDES, C. R.; MOURÃO, G. B.; COUTINHO, L. L.; REECY, J. M.; REGITANO, L. C. de A. Differentially expressed miRNAs in the stool of Bos indicus divergent for feed efficiency. Italian Journal of Animal Science, v. 22, supplement 1, 2023. p. 193-194. Congress of the Animal Science and Production Association, 25., Monopoli (BARI – ITALY), June 13–16, 2023.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
18. | | DINIZ, W. J. S.; MAZZONI, G.; COUTINHO; BANERJEE, P.; GEISTLINGER, L.; CESAR, A. S. M.; BERTOLINI. F.; AFONSO, J.; OLIVEIRA, P. S. N.; TIZIOTO, P. C.; KADARMIDEEN, H. N.; REGITANO, L. C. de A. Detection of Co-expressed pathway modules associated with mineral concentration and meat quality in nelore cattle. Frontiers in genetics, v. 10, n. 210, p. 1-12, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
19. | | SOMAVILLA, A. L.; REGITANO, L. C. de A.; ROSA, G. J. M.; MOKRY, F. B.; MUDADU, M. de A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; SOUZA, M. M. de; COUTINHO, L. L.; MUNARI, D. P. Genome-enabled prediction of breeding values for feedlot average daily weight gain in nelore cattle. G3: Genes, Genomes, Genetics, v. 7, p. 1-17, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
| |
20. | | BUSS, C. E.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; MUDADU, M. de A.; CESAR, A. S. M.; VENTURA, R. V.; AFONSO, J.; LIMA, A. O. de; COUTINHO, L. L.; TULLIO, R. R.; REGITANO, L. C. de A. Genome-wide efficient mixed-model study for meat quality in Nellore cattle. Journal of Animal Science, v. 94, e-suppl. 5; Journal of Dairy Science, v. 99, e-suppl. 1, p. 159, jul. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
Registros recuperados : 73 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|