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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
17/09/2020 |
Data da última atualização: |
16/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
NERY, F. M. B.; MELO, F. L.; BOITEUX, L. S.; RIBEIRO, S. da G.; RESENDE, R. O.; ORÍLIO, A. F.; BATISTA, J. G.; LIMA, M. F.; PEREIRA-CARVALHO, R. C. |
Afiliação: |
FLÁVIA M. B. NERY, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; LEONARDO SILVA BOITEUX, CNPH; SIMONE DA GRACA RIBEIRO, Cenargen; RENATO O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANELISE F. ORÍLIO, UNIVERSIDADE DE BRASÍLIA; JOSIANE G. BATISTA, UNIVERSIDADE DE BRASÍLIA; MIRTES FREITAS LIMA, CNPH; RITA C. PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA. |
Título: |
Molecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the Order Picornavirales. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Viruses, v. 12, n. 9, 950, 2020 |
DOI: |
https://doi.org/10.3390/v12090950 |
Idioma: |
Inglês |
Conteúdo: |
In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. MenosIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel viru... Mostrar Tudo |
Palavras-Chave: |
HDaV1; HDaV2; Virome. |
Thesagro: |
Hovenia Dulcis. |
Thesaurus Nal: |
Metagenomics; Picornavirales; Viruses. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219297/1/ARTIGO-MolecularCharacterizationHoveniaDulcis.pdf
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Marc: |
LEADER 02445naa a2200313 a 4500 001 2128264 005 2020-12-16 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/v12090950$2DOI 100 1 $aNERY, F. M. B. 245 $aMolecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2)$bnew tentative species within the Order Picornavirales.$h[electronic resource] 260 $c2020 520 $aIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. 650 $aMetagenomics 650 $aPicornavirales 650 $aViruses 650 $aHovenia Dulcis 653 $aHDaV1 653 $aHDaV2 653 $aVirome 700 1 $aMELO, F. L. 700 1 $aBOITEUX, L. S. 700 1 $aRIBEIRO, S. da G. 700 1 $aRESENDE, R. O. 700 1 $aORÍLIO, A. F. 700 1 $aBATISTA, J. G. 700 1 $aLIMA, M. F. 700 1 $aPEREIRA-CARVALHO, R. C. 773 $tViruses$gv. 12, n. 9, 950, 2020
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registros recuperados : 55 | |
9. | | GUIMARÃES, L. A.; DUSI, D. M. A.; SILVEIRA, E. D.; CARNEIRO, V. T. C. Análise da expressão de genes do tipo mads-box em Brachiaria brizantha (A. Rich.) Stapf por qRT-PCR e hibridização in situ. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 48.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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11. | | SILVEIRA, E. D.; GUIMARÃES, L. A.; MARTINS, N. F.; SILVA, F. R.; CARNEIRO, V. T. C. Análise de est´s de bibliotecas de ovário de Brachiaria brizantha apomítica e sexual em diferentes estágios de desenvolvimento. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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12. | | GUIMARÃES, L. A.; SILVEIRA, E. D.; SILVA, F. R.; CARNEIRO, V. T. C. Análise de bibliotecas de cDNA de ovários de B. brizantha apomítica e sexual em megasporogênese e megagametogênese (Analysis of cDNA libraries of ovaries from apomitic and sexual Brachiaria brizantha in megasporogenesis and megagametogenesis). In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 596.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | GUIMARÃES, L. A.; SILVEIRA, E. D.; SILVA, F. R.; CARNEIRO, V. T. C. Análise de bibliotecas de cdna de ovários de Brachiaria brizantha apomítica e sexual em megasporogênese e megagametogênese. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 45.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | SILVEIRA, E. D.; GUIMARÃES, L. A.; ALVES FERREIRA, M.; CARNEIRO, V. T. de C. Identification of the best reference genes for qPCR in sexual and apomictic B. brizantha. In: CONGRESSO INTERNACIONAL DE REPRODUÇÃO SEXUAL EM PLANTAS, 20., 2008, Brasília, DF. [Anais...]. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 240 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 259). p. 204.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | GUIMARÃES, L. A.; SILVEIRA, E. D.; DUSI, D. M. A.; CARNEIRO, V. T. C. Descrição de seqüências homólogas ao gene homeótico AGAMOUS em Brachiaria brizantha. In: CONGRESSO BRASILEIRO DE GENÉTICA, 52., 2006, Foz do Iguaçu, PR. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. p. 1285.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | GUIMARÃES, L. A.; SILVEIRA, E. D.; DUSI, D. M. A; CARNEIRO, V. T. C. Descrição de seqüências homólogas ao gene homeótico Agamous em Brachiaria brizantha. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 76.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | GRYNBERG, P.; GUIMARÃES, L. A.; COSTA, M. M. C.; TOGAWA, R. C.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Comprehensive profiling and characterization of Arachis stenosperma (peanut) and Meloidogyne arenaria (plantroot nematode) smallRNAs identified during the course of the infection. In: INTERNATIONAL CONFERENCE OF THE AB3C, 12., 2016, Belo Horizonte. [Proceedings...] [S.l.]: AB3C, 2016. X-meeting 2016.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 55 | |
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