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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
14/10/2020 |
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Data da última atualização: |
30/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TERRA. L. R.; FERREIRA, M. M. C.; TERAO, D.; QUEIROZ, S. C. do N. de. |
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Afiliação: |
LARISSA ROCHA TERRA, IQ-UNICAMP; MARCIA MIGUEL CASTRO FERREIRA, IQ-UNICAMP; DANIEL TERAO, CNPMA; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA. |
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Título: |
Otimização do processo não invasivo e não destrutivo de extração e análise de metabólitos voláteis de mamão papaia por SPME-GC-MS. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Química Nova, v. 43, n. 9, p. 1240-1245, 2020. |
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ISSN: |
1678-7064 |
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DOI: |
http://dx.doi.org/10.21577/0100-4042.20170611 |
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Idioma: |
Português |
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Conteúdo: |
Abstract: The analysis of volatiles is an alternative in the routine of quality control of fruits, since the volatile metabolites can change in the presence of microorganisms. A well-established method for extracting volatiles is the solid phase microextraction (SPME). This work aims to define some parameters (fiber coating, conditioning time and fiber exposure time) for an effective extraction. Whole papaya was inserted into a hermetically closed glass bottle and the system was left in conditioning for the volatiles accumulation. Then, extraction by SPME and subsequent desorption in a gas chromatograph was performed. Two fibers were evaluated: a 100 μm polydimethylsiloxane (PDMS) and a 65 μm polydimethylsiloxane/divinylbenzene (PDMS/DVB). The PDMS/DVB fiber extracted the highest number of volatiles. Central composite design (CCD) was performed to assess the effect of papaya conditioning time and the fiber exposure time on the response: number of detectable peaks. Both variables studied and their interactions were significant for the response. ANOVA showed that the quadratic terms and the lack of fit were not significant (p <0.05). A conditioning time of 10 min and exposure time of 30 min was sufficient for the detection of more than 100 compounds. |
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Palavras-Chave: |
Central composite design; Fruit; Response surface methodology Metabolomics. |
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Thesagro: |
Análise Química; Carica Papaya; Cromatografia Gasosa; Espectrometria; Mamão; Microrganismo. |
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Thesaurus Nal: |
Chemical analysis; Gas chromatography; Mass spectrometry; Metabolites; Microbial detection; Papayas; Solid phase microextraction; Volatile organic compounds. |
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Categoria do assunto: |
H Saúde e Patologia
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218363/1/Terao-Optimization-extraction-2020.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216646/1/Terao-Otimizacao-processo-2020.pdf
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Marc: |
LEADER 02491naa a2200385 a 4500
001 2127193
005 2020-11-30
008 2020 bl uuuu u00u1 u #d
022 $a1678-7064
024 7 $ahttp://dx.doi.org/10.21577/0100-4042.20170611$2DOI
100 1 $aTERRA. L. R.
245 $aOtimização do processo não invasivo e não destrutivo de extração e análise de metabólitos voláteis de mamão papaia por SPME-GC-MS.$h[electronic resource]
260 $c2020
520 $aAbstract: The analysis of volatiles is an alternative in the routine of quality control of fruits, since the volatile metabolites can change in the presence of microorganisms. A well-established method for extracting volatiles is the solid phase microextraction (SPME). This work aims to define some parameters (fiber coating, conditioning time and fiber exposure time) for an effective extraction. Whole papaya was inserted into a hermetically closed glass bottle and the system was left in conditioning for the volatiles accumulation. Then, extraction by SPME and subsequent desorption in a gas chromatograph was performed. Two fibers were evaluated: a 100 μm polydimethylsiloxane (PDMS) and a 65 μm polydimethylsiloxane/divinylbenzene (PDMS/DVB). The PDMS/DVB fiber extracted the highest number of volatiles. Central composite design (CCD) was performed to assess the effect of papaya conditioning time and the fiber exposure time on the response: number of detectable peaks. Both variables studied and their interactions were significant for the response. ANOVA showed that the quadratic terms and the lack of fit were not significant (p <0.05). A conditioning time of 10 min and exposure time of 30 min was sufficient for the detection of more than 100 compounds.
650 $aChemical analysis
650 $aGas chromatography
650 $aMass spectrometry
650 $aMetabolites
650 $aMicrobial detection
650 $aPapayas
650 $aSolid phase microextraction
650 $aVolatile organic compounds
650 $aAnálise Química
650 $aCarica Papaya
650 $aCromatografia Gasosa
650 $aEspectrometria
650 $aMamão
650 $aMicrorganismo
653 $aCentral composite design
653 $aFruit
653 $aResponse surface methodology Metabolomics
700 1 $aFERREIRA, M. M. C.
700 1 $aTERAO, D.
700 1 $aQUEIROZ, S. C. do N. de
773 $tQuímica Nova$gv. 43, n. 9, p. 1240-1245, 2020.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
