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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pesca e Aquicultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/11/2020 |
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Data da última atualização: |
22/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARRIERO, M. M.; HENRIQUE SILVA, F.; MEIRA, C. M.; GATO, I. M. Q.; CAETANO, A. R.; LOBO, F. P.; ALVES, A. L.; VARELA, E. S.; MAIA, A. A. M. |
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Afiliação: |
MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP. |
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Título: |
Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 43, n. 4, e20200006, 2020. |
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ISSN: |
1678-4685 |
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DOI: |
https://doi.org/10.1590/1678-4685-gmb-2020-0006 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract. In the present study, the complete characterization of cDNA and genomic sequences of IL-1 and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1 cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1 and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups |
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Palavras-Chave: |
Bacterial infection; Expressão gênica; Fish immunology; Imunologia de peixe; Infecção bacteriana; Interleukin; South American fish. |
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Thesagro: |
Infecção; Pacu; Peixe. |
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Thesaurus Nal: |
Fish; Gene expression; Interleukins; Piaractus mesopotamicus. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220516/1/gmb-2020.pdf
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Marc: |
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022 $a1678-4685
024 7 $ahttps://doi.org/10.1590/1678-4685-gmb-2020-0006$2DOI
100 1 $aCARRIERO, M. M.
245 $aMolecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.$h[electronic resource]
260 $c2020
520 $aAbstract. In the present study, the complete characterization of cDNA and genomic sequences of IL-1 and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1 cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1 and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups
650 $aFish
650 $aGene expression
650 $aInterleukins
650 $aPiaractus mesopotamicus
650 $aInfecção
650 $aPacu
650 $aPeixe
653 $aBacterial infection
653 $aExpressão gênica
653 $aFish immunology
653 $aImunologia de peixe
653 $aInfecção bacteriana
653 $aInterleukin
653 $aSouth American fish
700 1 $aHENRIQUE SILVA, F.
700 1 $aMEIRA, C. M.
700 1 $aGATO, I. M. Q.
700 1 $aCAETANO, A. R.
700 1 $aLOBO, F. P.
700 1 $aALVES, A. L.
700 1 $aVARELA, E. S.
700 1 $aMAIA, A. A. M.
773 $tGenetics and Molecular Biology$gv. 43, n. 4, e20200006, 2020.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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