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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
10/11/2020 |
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Data da última atualização: |
10/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAVALCANTE, G. S.; MORAIS, S. M. de; ANDRÉ, W. P. P.; ARAÚJO FILHO, J. V. de; MUNIZ, C. R.; ROCHA, L. O. da; RIBEIRO, W. L. C.; RODRIGUES, A. L. M.; OLIVEIRA, L. M. B. de; BEVILAQUA, C. M. L.; RAMOS, M. V. |
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Afiliação: |
GÉSSICA SOARES CAVALCANTE, Laboratório de Química de Produtos Naturais, Núcleo de Pesquisa em Sanidade Animal, Universidade Estadual do Ceará; SELENE MAIA DE MORAIS, Laboratório de Química de Produtos Naturais, Núcleo de Pesquisa em Sanidade Animal, Universidade Estadual do Ceará; Laboratório de Análises Cromatográficas e Espectroscópicas UECE; WEIBSON PAZ PINHEIRO ANDRÉ, Laboratório de Doenças Parasitárias, Núcleo de Pesquisa em Sanidade Animal, Universidade Estadual do Ceará; JOSÉ VILEMAR DE ARAÚJO FILHO, Laboratório de Doenças Parasitárias, Núcleo de Pesquisa em Sanidade Animal, Universidade Estadual do Ceará; CELLI RODRIGUES MUNIZ, CNPAT; LETÍCIA OLIVEIRA DA ROCHA, Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará; WESLEY LYEVERTON CORREIA RIBEIRO, Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará; ANA LIVYA MOREIRA RODRIGUES, Laboratório de Análises Cromatográficas e Espectroscópicas, Centro de Ciências e Tecnologia, Universidade Estadual do Ceará; LORENA MAYANA BESERRA DE OLIVEIRA, Laboratório de Doenças Parasitárias, Núcleo de Pesquisa em Sanidade Animal, Universidade Estadual do Ceará; CLAUDIA MARIA LEAL BEVILAQUA, Laboratório de Doenças Parasitárias, Núcleo de Pesquisa em Sanidade Animal, Universidade Estadual do Ceará; MÁRCIO VIANA RAMOS, Laboratório de Bioquímica e Biologia de Proteínas Vegetais, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará. |
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Título: |
Chemical constituents of calotropis procera latex and ultrastructural effects on haemonchus contortus. |
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Título original: |
Constituintes químicos do látex de calotropis procera e efeitos ultraestruturais em haemonchus contortus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinaria, v. 29, n. 2, article number e001320, p. 1-12, 2020. |
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DOI: |
DOI: 10.1590/S1984-29612020045 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Cutícula; Latex protein; Metabólitos secundários; Proteínas do látex. |
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Thesagro: |
Anti-Helmíntico; Haemonchus Contortus; Nematóide. |
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Thesaurus Nal: |
Animal cuticle; Animal parasitic nematodes; Anthelmintics; Secondary metabolites. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217622/1/ART20087.pdf
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Marc: |
LEADER 01326naa a2200385 a 4500
001 2126444
005 2020-11-10
008 2020 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1590/S1984-29612020045$2DOI
100 1 $aCAVALCANTE, G. S.
240 $aConstituintes químicos do látex de calotropis procera e efeitos ultraestruturais em haemonchus contortus.
245 $aChemical constituents of calotropis procera latex and ultrastructural effects on haemonchus contortus.$h[electronic resource]
260 $c2020
650 $aAnimal cuticle
650 $aAnimal parasitic nematodes
650 $aAnthelmintics
650 $aSecondary metabolites
650 $aAnti-Helmíntico
650 $aHaemonchus Contortus
650 $aNematóide
653 $aCutícula
653 $aLatex protein
653 $aMetabólitos secundários
653 $aProteínas do látex
700 1 $aMORAIS, S. M. de
700 1 $aANDRÉ, W. P. P.
700 1 $aARAÚJO FILHO, J. V. de
700 1 $aMUNIZ, C. R.
700 1 $aROCHA, L. O. da
700 1 $aRIBEIRO, W. L. C.
700 1 $aRODRIGUES, A. L. M.
700 1 $aOLIVEIRA, L. M. B. de
700 1 $aBEVILAQUA, C. M. L.
700 1 $aRAMOS, M. V.
773 $tRevista Brasileira de Parasitologia Veterinaria$gv. 29, n. 2, article number e001320, p. 1-12, 2020.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Solos. |
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Data corrente: |
06/02/2015 |
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Data da última atualização: |
06/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S. |
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Afiliação: |
D. C. A. LEITE, UFRJ; FABIANO DE CARVALHO BALIEIRO, CNPS; C. A. PIRES; BEATA EMOKE MADARI, CNPAF; A. S. ROSADO, UFRJ; HEITOR LUIZ DA COSTA COUTINHO, CNPS; R. S. PEIXOTO, UFRJ. |
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Título: |
Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014. |
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DOI: |
10.1590/S1517-83822014000100023 |
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Idioma: |
Inglês |
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Conteúdo: |
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples. MenosMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-... Mostrar Tudo |
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Palavras-Chave: |
Comunidades microbianas; Extração de DNA; Índice de pureza do DNA; PCR-DGGE. |
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Thesaurus NAL: |
biochar. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117433/1/BJMv45n1a23.pdf
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Marc: |
LEADER 02342naa a2200265 a 4500
001 2007986
005 2015-02-06
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1590/S1517-83822014000100023$2DOI
100 1 $aLEITE, D. C. A.
245 $aComparison of DNA extraction protocols for microbial communities from soil treated with biochar.$h[electronic resource]
260 $c2014
520 $aMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples.
650 $abiochar
653 $aComunidades microbianas
653 $aExtração de DNA
653 $aÍndice de pureza do DNA
653 $aPCR-DGGE
700 1 $aBALIEIRO, F. C.
700 1 $aC. A. PIRES
700 1 $aMADARI, B. E.
700 1 $aROSADO, A. S.
700 1 $aCOUTINHO, H. L. C.
700 1 $aPEIXOTO, R. S.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 45, n. 1, p. 175-183, 2014.
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Registro original: |
Embrapa Solos (CNPS) |
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