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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
06/07/2020 |
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Data da última atualização: |
18/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVES, J. N.; ARAUJO, G. G. L. de; PEREIRA, L. G. R.; GONZAGA NETO, S.; MENEZES, D. R.; VOLTOLINI, T. V.; GOIS, G. C.; SOUZA, L. C.; CAMPOS, F. S. |
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Afiliação: |
J. N. Alves; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; S. Gonzaga Neto; D. R. Menezes; TADEU VINHAS VOLTOLINI, CPATSA; G. C. Gois; L. C. Souza; F. S. Campos. |
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Título: |
Incorporating oldman saltbush hay and prickly pear in diets for red Sindhi calves. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
South African Journal of Animal Science, v. 50, n. 3, p. 403-414, 2020. |
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DOI: |
http://dx.doi.org/10.4314/sajas.v50i3.7 |
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Idioma: |
Inglês |
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Conteúdo: |
This study was designed to evaluate the nutrient intake, digestibility, degradability, and ruminal characteristics of Sindhi heifers fed diets that contained a combined total of 75% oldman saltbush hay (hereafter saltbush hay) and prickly pear cactus. Eight 12-month old intact male red Sindhi calves (four fistulated and four non-fistulated) with an initial mean weight of 170 ± 5 kg were assigned to 4 × 4 Latin squares, where factors consisted of four diets, namely 15% hay and 60% cactus; 30% hay and 45% cactus; 45% hay and 30% cactus; and 60% hay and 15% cactus, and four times at which rumen fluid was collected. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) intakes in kg/day and NDF in percentage live weight (LW), water intake, salinity, and conductivity increased with hay level. Intake and digestibility of nonfibrous carbohydrates were curvilinear with higher values in diets containing 30% saltbush hay. The apparent digestibility of dry matter (DM) and organic matter decreased linearly as the concentration of saltbush hay increased in the diet. The pH of the rumen fluid was within the acceptable range for favourable microbial growth. The low temperature and high salinity and conductivity indices in the diets should be viewed with caution at higher concentrations of saltbush hay, because of a possible decrease in nutrient absorption and the development of health problems in the animals. Apparent degradability coefficients of DM and NDF were affected significantly by inclusion of prickly pear and saltbush hay in the diets. MenosThis study was designed to evaluate the nutrient intake, digestibility, degradability, and ruminal characteristics of Sindhi heifers fed diets that contained a combined total of 75% oldman saltbush hay (hereafter saltbush hay) and prickly pear cactus. Eight 12-month old intact male red Sindhi calves (four fistulated and four non-fistulated) with an initial mean weight of 170 ± 5 kg were assigned to 4 × 4 Latin squares, where factors consisted of four diets, namely 15% hay and 60% cactus; 30% hay and 45% cactus; 45% hay and 30% cactus; and 60% hay and 15% cactus, and four times at which rumen fluid was collected. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) intakes in kg/day and NDF in percentage live weight (LW), water intake, salinity, and conductivity increased with hay level. Intake and digestibility of nonfibrous carbohydrates were curvilinear with higher values in diets containing 30% saltbush hay. The apparent digestibility of dry matter (DM) and organic matter decreased linearly as the concentration of saltbush hay increased in the diet. The pH of the rumen fluid was within the acceptable range for favourable microbial growth. The low temperature and high salinity and conductivity indices in the diets should be viewed with caution at higher concentrations of saltbush hay, because of a possible decrease in nutrient absorption and the development of health problems in the animals. Apparent degradability coefficients of DM and NDF were affected significan... Mostrar Tudo |
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Palavras-Chave: |
Oldbush; Raça Sindi; Semiárido. |
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Thesagro: |
Digestibilidade Aparente; Nutrição Animal. |
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Thesaurus Nal: |
Animal nutrition; Atriplex nummularia; Opuntia ficus-indica. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214444/1/Incorporating-oldman-saltbush-hay-and-prickly-pear-in-diets-for-red-Sindhi-calves-2020.pdf
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Marc: |
LEADER 02538naa a2200325 a 4500
001 2123645
005 2021-02-18
008 2020 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4314/sajas.v50i3.7$2DOI
100 1 $aALVES, J. N.
245 $aIncorporating oldman saltbush hay and prickly pear in diets for red Sindhi calves.$h[electronic resource]
260 $c2020
520 $aThis study was designed to evaluate the nutrient intake, digestibility, degradability, and ruminal characteristics of Sindhi heifers fed diets that contained a combined total of 75% oldman saltbush hay (hereafter saltbush hay) and prickly pear cactus. Eight 12-month old intact male red Sindhi calves (four fistulated and four non-fistulated) with an initial mean weight of 170 ± 5 kg were assigned to 4 × 4 Latin squares, where factors consisted of four diets, namely 15% hay and 60% cactus; 30% hay and 45% cactus; 45% hay and 30% cactus; and 60% hay and 15% cactus, and four times at which rumen fluid was collected. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) intakes in kg/day and NDF in percentage live weight (LW), water intake, salinity, and conductivity increased with hay level. Intake and digestibility of nonfibrous carbohydrates were curvilinear with higher values in diets containing 30% saltbush hay. The apparent digestibility of dry matter (DM) and organic matter decreased linearly as the concentration of saltbush hay increased in the diet. The pH of the rumen fluid was within the acceptable range for favourable microbial growth. The low temperature and high salinity and conductivity indices in the diets should be viewed with caution at higher concentrations of saltbush hay, because of a possible decrease in nutrient absorption and the development of health problems in the animals. Apparent degradability coefficients of DM and NDF were affected significantly by inclusion of prickly pear and saltbush hay in the diets.
650 $aAnimal nutrition
650 $aAtriplex nummularia
650 $aOpuntia ficus-indica
650 $aDigestibilidade Aparente
650 $aNutrição Animal
653 $aOldbush
653 $aRaça Sindi
653 $aSemiárido
700 1 $aARAUJO, G. G. L. de
700 1 $aPEREIRA, L. G. R.
700 1 $aGONZAGA NETO, S.
700 1 $aMENEZES, D. R.
700 1 $aVOLTOLINI, T. V.
700 1 $aGOIS, G. C.
700 1 $aSOUZA, L. C.
700 1 $aCAMPOS, F. S.
773 $tSouth African Journal of Animal Science$gv. 50, n. 3, p. 403-414, 2020.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
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Origem |
Tipo/Formato |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Uva e Vinho. |
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Data corrente: |
21/10/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AGUSTINI, B. C.; SILVA, L. P. da; BLOCH JUNIOR, C.; BONFIM, T. M. B.; SILVA, G. A. da. |
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Afiliação: |
BRUNA CARLA AGUSTINI, CNPUV; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, CENARGEN; GILDO ALMEIDA DA SILVA, CNPUV. |
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Título: |
Evaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 98, n. 12, p. 5645-5654, 2014. |
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Idioma: |
Português |
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Notas: |
DOI: 10.1007/s00253-014-5686-7 |
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Conteúdo: |
Yeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand. Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning tomass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated fromgrape must and 67.7% of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After matching the correct taxon for these strains by using molecular biology approaches, the spectra concerning the missing species were added in a supplementary database. This new library was able to accurately predict unidentified species at first instance by MALDI-TOF MS, proving it is a powerful tool for the identification of environmental yeasts. MenosYeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand. Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning tomass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated fromgrape must and 67.7% of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After ma... Mostrar Tudo |
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Palavras-Chave: |
Levedura; MALDI-TOF MS. |
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Thesagro: |
Base de dados; Biologia molecular; Genética; Levedo; Microrganismo. |
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Thesaurus NAL: |
Molecular biology; Yeasts. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110284/1/Silva-AMB.pdf
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Marc: |
LEADER 02726naa a2200289 a 4500
001 1997892
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aAGUSTINI, B. C.
245 $aEvaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database.$h[electronic resource]
260 $c2014
500 $aDOI: 10.1007/s00253-014-5686-7
520 $aYeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand. Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning tomass spectrometry, data available for the differentiation of yeast species for industrial purpose is limited and reference databases commercially available comprise almost exclusively clinical microorganisms. In this context, studies focusing on environmental isolates are required to extend the existing databases. The development of a supplementary database and the assessment of a commercial database for taxonomic identifications of environmental yeast are the aims of this study. We challenge MALDI-TOF MS to create protein profiles for 845 yeast strains isolated fromgrape must and 67.7% of the strains were successfully identified according to previously available manufacturer database. The remaining 32.3 % strains were not identified due to the absence of a reference spectrum. After matching the correct taxon for these strains by using molecular biology approaches, the spectra concerning the missing species were added in a supplementary database. This new library was able to accurately predict unidentified species at first instance by MALDI-TOF MS, proving it is a powerful tool for the identification of environmental yeasts.
650 $aMolecular biology
650 $aYeasts
650 $aBase de dados
650 $aBiologia molecular
650 $aGenética
650 $aLevedo
650 $aMicrorganismo
653 $aLevedura
653 $aMALDI-TOF MS
700 1 $aSILVA, L. P. da
700 1 $aBLOCH JUNIOR, C.
700 1 $aBONFIM, T. M. B.
700 1 $aSILVA, G. A. da
773 $tApplied Microbiology and Biotechnology$gv. 98, n. 12, p. 5645-5654, 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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