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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
23/04/2019 |
Data da última atualização: |
06/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. |
Afiliação: |
SILVIA DE OLIVEIRA DORTA, ESALQ; JULIANA BALBINOTTE, Centro de Citricultura Sylvio Moreira; ROSE MONNERAT, ESALQ; JOAO ROBERTO SPOTTI LOPES, Centro de Citricultura Sylvio Moreira; TATIANE DA CUNHA, ESALQ; ODIMAR ZANUZO ZANARDI, Fundecitrus; MARCELO PEDREIRA DE MIRANDA, Fundecitrus; MARCOS ANTONIO MACHADO, Centro de Citricultura Sylvio Moreira; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Insect Science, p.1-12, 2019. |
ISSN: |
1744-7917 |
Idioma: |
Inglês |
Conteúdo: |
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. MenosBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for... Mostrar Tudo |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Thesaurus Nal: |
Citrus; Diaphorina citri; Plant diseases and disorders. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196193/1/Dorta-et-al-2019-Insect-Science.pdf
|
Marc: |
LEADER 02385naa a2200289 a 4500 001 2108421 005 2019-12-06 008 2019 bl uuuu u00u1 u #d 022 $a1744-7917 100 1 $aDORTA, S. de O. 245 $aSelection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera$bLiviidae) nymphs.$h[electronic resource] 260 $c2019 520 $aBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. 650 $aCitrus 650 $aDiaphorina citri 650 $aPlant diseases and disorders 650 $aDoença de Planta 650 $aFruta Cítrica 700 1 $aBALBINOTTE, J. 700 1 $aMONNERAT, R. 700 1 $aLOPES, J. R. S. 700 1 $aCUNHA, T. da 700 1 $aZANARDI, O. Z. 700 1 $aMIRANDA, M. P. de 700 1 $aMACHADO, M. A. 700 1 $aASTUA, J. de F. 773 $tInsect Science, p.1-12, 2019.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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101. | | SALTON, J. C.; TOMAZI, M.; COMUNELLO, E.; ZANATTA, J. A.; RABELLO, L. M. Condutividade elétrica e atributos físicos e químicos de um Latossolo após 15 anos sob sistemas de manejo em Mato Grosso do Sul. In: INAMASU, R. Y.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; BERNARDI, A. C. de C. (Ed.). Agricultura de precisão: um novo olhar. São Carlos: Embrapa Instrumentação, 2011. p. 254-260.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Florestas; Embrapa Instrumentação. |
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103. | | RIBAS, H. S.; FLUMIGNAN, D. L.; SILVA, C. J. da; COMUNELLO, E.; GEISENHOFF, L. O. Determinação do coeficiente de cultivo (Kc) da cana-de-açúcar para a região de Dourados, MS. In: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2019, Dourados. Resumos... Brasília, DF: Embrapa, 2019. JIPE 2019Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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109. | | MOTTA, I. de S.; COMUNELLO, E.; ITO, M. A.; OBA, G. C.; BITENCOURT, I. A. N. Produtividade do maracujazeiro-azedo em função de idades da muda. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 27.; ENCONTRO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 17., 2022, Florianópolis, SC. Anais... + Ciência + Saúde. Florianópolis, SC: Epagri, 2022.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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110. | | JUNQUEIRA, P. H.; SALTON, J. C.; TOMAZI, M.; COMUNELLO, E.; CASTILHO, L. Qualidade estrutural do solo em diferentes sistemas de manejo no município de Ponta Porã, MS In: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2018, Dourados. Resumos... Brasília, DF: Embrapa, 2018. JIPE 2018Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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112. | | FIETZ, C. R.; LAMAS, F. M.; COMUNELLO, E.; FLUMIGNAN, D. L. Risco climático para o algodoeiro na região de Dourados, MS, em diferentes épocas de semeadura. In: CONGRESSO BRASILEIRO DO ALGODÃO, 12., 2019, Goiânia, GO. Anais... Goiânia: Abrapa, 2019.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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114. | | GONÇALVES, G. G. G.; DANIEL, O.; COMUNELLO, E.; ARAI, F. K.; VITORINO, A. C. T. Evolução do uso e cobertura do solo na Bacia Hidrográfica do Rio Dourados-MS, Brasil. Caminhos de Geografia: Revista on line, v. 11, n. 36, p. 366-374, dez. 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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116. | | SMALING, E. M. A.; ROSCOE, R.; LESSCHEN, J. P.; BOUWMAN, A. F.; COMUNELLO, E. From forest to wate: Assessment of the Brazilian soybean chain, using nitrogen as a marker. Agriculture, Ecosystems and Envivonment, v. 128, n. 3, p. 185-197, Nov. 2008.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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120. | | FIETZ, C. R.; COMUNELLO, E.; FLUMIGNAN, D. L.; LAMAS, F. M. Época de semeadura do algodoeiro na região de Dourados, MS, com base no risco climático. In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 21., 2019, Catalão. Anais... Catalão: CBAGRO: UFGO, 2019. p. 1196-1200Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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Registros recuperados : 166 | |
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