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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/09/2018 |
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Data da última atualização: |
29/11/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ELOY, A. M. X. |
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Afiliação: |
ANGELA MARIA XAVIER ELOY, CNPC. |
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Título: |
Proteins candidates for complementary diagnosis of goats naturally infected by Lentivirus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Virology & Mycology, v. 7, p. 53, 2018. Edição dos Joint Event 10th International Virology Summit; 4th International Conference on Influenza & Zoonotic Diseases |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The caprine arthritis encephalitis (CAE) is a disease caused by Lentivirus genus, Orthoretrovirinae subfamily, and Retroviridae family belonging to the same human immunodeficiency virus (HIV) family. This viral disease is widespread in the world, affecting especially goat dairy, being easily transmitted by contact among animals secretion, milk ingestion and also through contaminated ejaculate. Has no cure and is difficult to control, since the tests in use are not accurate due to virus latency, providing false negative results. The present study aimed to identify the major seminal plasma protein profile of goats chronically infected by CAE. Two groups containing five males each, aging 4 to 5 years were used. The first group was composed by naturally and chronically CAE-infected animals and the control by seronegative, both confirmed by two blood tests of Western blotting (WB) and by polymerase chain reaction (PCR). The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. The proteins of high expression identified only in seropositive animals play an important role in the viral infection, such as the protease arylsulfatase A, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. These results show that seminal plasma proteins are involved on reproductive process protection in chronically infected goats by CAE. As the arylsulfatase A enzyme participates in the physiological events of fertilization in bulls and sheep, and it is absent in seronegative goat to CAEV, probably the main function of this enzyme in goats can be related to metabolism control of sulfatides, involved to virus control. MenosAbstract: The caprine arthritis encephalitis (CAE) is a disease caused by Lentivirus genus, Orthoretrovirinae subfamily, and Retroviridae family belonging to the same human immunodeficiency virus (HIV) family. This viral disease is widespread in the world, affecting especially goat dairy, being easily transmitted by contact among animals secretion, milk ingestion and also through contaminated ejaculate. Has no cure and is difficult to control, since the tests in use are not accurate due to virus latency, providing false negative results. The present study aimed to identify the major seminal plasma protein profile of goats chronically infected by CAE. Two groups containing five males each, aging 4 to 5 years were used. The first group was composed by naturally and chronically CAE-infected animals and the control by seronegative, both confirmed by two blood tests of Western blotting (WB) and by polymerase chain reaction (PCR). The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. The proteins of high expression identified only in seropositive animals play an important role in the viral infection, such as the protease arylsulfatase A, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like i... Mostrar Tudo |
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Palavras-Chave: |
CAE. |
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Thesaurus Nal: |
Caprine arthritis-encephalitis; Goats; Lentivirus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183042/1/CNPC-2018-Proteins.pdf
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Marc: |
LEADER 02656nam a2200157 a 4500
001 2095765
005 2023-11-29
008 2018 bl uuuu u00u1 u #d
100 1 $aELOY, A. M. X.
245 $aProteins candidates for complementary diagnosis of goats naturally infected by Lentivirus.$h[electronic resource]
260 $aVirology & Mycology, v. 7, p. 53, 2018. Edição dos Joint Event 10th International Virology Summit; 4th International Conference on Influenza & Zoonotic Diseases$c2018
520 $aAbstract: The caprine arthritis encephalitis (CAE) is a disease caused by Lentivirus genus, Orthoretrovirinae subfamily, and Retroviridae family belonging to the same human immunodeficiency virus (HIV) family. This viral disease is widespread in the world, affecting especially goat dairy, being easily transmitted by contact among animals secretion, milk ingestion and also through contaminated ejaculate. Has no cure and is difficult to control, since the tests in use are not accurate due to virus latency, providing false negative results. The present study aimed to identify the major seminal plasma protein profile of goats chronically infected by CAE. Two groups containing five males each, aging 4 to 5 years were used. The first group was composed by naturally and chronically CAE-infected animals and the control by seronegative, both confirmed by two blood tests of Western blotting (WB) and by polymerase chain reaction (PCR). The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. The proteins of high expression identified only in seropositive animals play an important role in the viral infection, such as the protease arylsulfatase A, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. These results show that seminal plasma proteins are involved on reproductive process protection in chronically infected goats by CAE. As the arylsulfatase A enzyme participates in the physiological events of fertilization in bulls and sheep, and it is absent in seronegative goat to CAEV, probably the main function of this enzyme in goats can be related to metabolism control of sulfatides, involved to virus control.
650 $aCaprine arthritis-encephalitis
650 $aGoats
650 $aLentivirus
653 $aCAE
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
11/07/2007 |
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Data da última atualização: |
03/01/2024 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
CARVALHO, K. J. M. de; FREIRE FILHO, F. R.; LOPES, A. C. de A.; ROCHA, M. de M.; GOMES, R. L. F.; SOUSA, I. de S. |
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Título: |
Controle genético do comprimento do pedúnculo em feijão-caupi. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 1.; REUNIÃO NACIONAL DE FEIJÃO-CAUPI, 6., 2006, Teresina. Tecnologias para o agronegócio: anais. Teresina: Embrapa Meio-Norte, 2006. |
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Descrição Física: |
1 CD-ROM. |
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Série: |
(Embrapa Meio-Norte. Documentos, 121). |
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Idioma: |
Português |
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Conteúdo: |
O estudo da herança do caráter comprimento do pedúnculo em feijão-caupi foi realizado através de estimativas dos parâmetros genéticos, baseando-se em médias e variâncias, a partir do cruzamento entre genótipos parentais TVx-5058-09C de pedúnculo curto e TR 96-282-22G de pedúnculo longo. Estes genótipos e as gerações F1, F2, RC1 (P1 x F1) e RC2 (P2 x F1) foram avaliados quanto ao caráter citado, num experimento de blocos casualizados com quatro repetições. Foram estimados valores para as variâncias fenotípica, genotípica, ambiental, aditiva, e devido à dominância; herdabilidades no sentido amplo e restrito; grau médio de dominância e número mínimo de genes determinando o caráter. O modelo aditivo-dominante foi suficiente para explicar a variação observada, o efeito gênico aditivo foi o mais importante no controle do caráter em estudo, sendo este controlado por cinco genes. O alto valor da herdabilidade no sentido restrito permite a seleção precoce para o comprimento do pedúnculo. |
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Palavras-Chave: |
Arquitetura da planta; Modelo aditivo-dominante. |
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Thesagro: |
Parâmetro Genético. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94336/1/GM31.pdf
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Marc: |
LEADER 01843nam a2200229 a 4500
001 1068802
005 2024-01-03
008 2006 bl uuuu u00u1 u #d
100 1 $aCARVALHO, K. J. M. de
245 $aControle genético do comprimento do pedúnculo em feijão-caupi.
260 $aIn: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 1.; REUNIÃO NACIONAL DE FEIJÃO-CAUPI, 6., 2006, Teresina. Tecnologias para o agronegócio: anais. Teresina: Embrapa Meio-Norte$c2006
300 $c1 CD-ROM.
490 $a(Embrapa Meio-Norte. Documentos, 121).
520 $aO estudo da herança do caráter comprimento do pedúnculo em feijão-caupi foi realizado através de estimativas dos parâmetros genéticos, baseando-se em médias e variâncias, a partir do cruzamento entre genótipos parentais TVx-5058-09C de pedúnculo curto e TR 96-282-22G de pedúnculo longo. Estes genótipos e as gerações F1, F2, RC1 (P1 x F1) e RC2 (P2 x F1) foram avaliados quanto ao caráter citado, num experimento de blocos casualizados com quatro repetições. Foram estimados valores para as variâncias fenotípica, genotípica, ambiental, aditiva, e devido à dominância; herdabilidades no sentido amplo e restrito; grau médio de dominância e número mínimo de genes determinando o caráter. O modelo aditivo-dominante foi suficiente para explicar a variação observada, o efeito gênico aditivo foi o mais importante no controle do caráter em estudo, sendo este controlado por cinco genes. O alto valor da herdabilidade no sentido restrito permite a seleção precoce para o comprimento do pedúnculo.
650 $aParâmetro Genético
653 $aArquitetura da planta
653 $aModelo aditivo-dominante
700 1 $aFREIRE FILHO, F. R.
700 1 $aLOPES, A. C. de A.
700 1 $aROCHA, M. de M.
700 1 $aGOMES, R. L. F.
700 1 $aSOUSA, I. de S.
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Embrapa Meio-Norte (CPAMN) |
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