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Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
13/09/2012 |
Data da última atualização: |
05/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
PINHEIRO, T. M.; ARAÚJO, L. G. de; SILVA-LOBO, V. L. da; PRABHU, A. S.; FILIPPI, M. C. de. |
Afiliação: |
THIAGO MARTINS PINHEIRO, UNIVERSIDADE FEDERAL DE GOIÁS; LEILA GARCÊS DE ARAÚJO, UNIVERSIDADE FEDERAL DE GOIÁS; VALACIA LEMES DA SILVA LOBO, CNPAF; ANNE SITARAMA PRABHU, CNPAF; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
Título: |
Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 12, n. 3, p. 164-170, jul./ago. 2012. |
Idioma: |
Inglês |
Conteúdo: |
The rice cultivar Cica-8 exhibit differential reaction to several pathotypes of Magnaporthe oryzae. The objective of the present investigation was to determine the number of alleles involved in the expression of resistance to leaf blast and identify microsatellite markers linked to these alleles. A cross between cultivar Metica-1 and Cica-8 susceptible and resistant, respectively, to pathotype IB-1 (Py1049) was made to obtain F1, F2, BC1:1 and BC1:2 progenies. Greenhouse tests for leaf blast reaction showed that resistance is controlled by a monogenic dominant gene. For testing microsatellite markers, DNA of both resistant and susceptible parents and F1 and F2 populations was extracted. As expected for single dominant gene the F2 populations segregated at a ratio of 3:1. Of the 11 microsatellite markers tested, one marker RM 7102 was found to be closely linked to the resistant allele at a distance of 2.7 cM, in the cultivar Cica-8 to pathotype IB-1. |
Thesagro: |
Arroz; Brusone; Oryza sativa. |
Thesaurus NAL: |
Genetic markers; Magnaporthe grisea; Marker-assisted selection; Rice. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66202/1/arttag.pdf
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Marc: |
LEADER 01728naa a2200253 a 4500 001 1933575 005 2022-05-05 008 2012 bl uuuu u00u1 u #d 100 1 $aPINHEIRO, T. M. 245 $aTagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8.$h[electronic resource] 260 $c2012 520 $aThe rice cultivar Cica-8 exhibit differential reaction to several pathotypes of Magnaporthe oryzae. The objective of the present investigation was to determine the number of alleles involved in the expression of resistance to leaf blast and identify microsatellite markers linked to these alleles. A cross between cultivar Metica-1 and Cica-8 susceptible and resistant, respectively, to pathotype IB-1 (Py1049) was made to obtain F1, F2, BC1:1 and BC1:2 progenies. Greenhouse tests for leaf blast reaction showed that resistance is controlled by a monogenic dominant gene. For testing microsatellite markers, DNA of both resistant and susceptible parents and F1 and F2 populations was extracted. As expected for single dominant gene the F2 populations segregated at a ratio of 3:1. Of the 11 microsatellite markers tested, one marker RM 7102 was found to be closely linked to the resistant allele at a distance of 2.7 cM, in the cultivar Cica-8 to pathotype IB-1. 650 $aGenetic markers 650 $aMagnaporthe grisea 650 $aMarker-assisted selection 650 $aRice 650 $aArroz 650 $aBrusone 650 $aOryza sativa 700 1 $aARAÚJO, L. G. de 700 1 $aSILVA-LOBO, V. L. da 700 1 $aPRABHU, A. S. 700 1 $aFILIPPI, M. C. de 773 $tCrop Breeding and Applied Biotechnology$gv. 12, n. 3, p. 164-170, jul./ago. 2012.
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Embrapa Arroz e Feijão (CNPAF) |
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