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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
03/01/2018 |
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Data da última atualização: |
03/01/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, L. V.; GOULARTE, N. A. de A.; MAGRIN, F. P; HAWERROTH, F. J.; SIMOES, F. |
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Afiliação: |
Lisiane Viaceli de Oliveira; Natália Aparecida de Almeida Goularte; Fernanda Pelizzari Magrin; FERNANDO JOSE HAWERROTH, CNPUV; Fabiano Simões. |
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Título: |
Manejo da poda verde em macieiras "Gala" em pomar sob tela antigranizo. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: SALÃO INTEGRADO DE ENSINO, PESQUISA E EXTENSÃO, 7.; JORNADA DE PÓS-GRADUAÇÃO, 3.; SEMINÁRIO SOBRE TERRITORIALIDADE, 2., 2017, Tapes, RS. Anais... Tapes, RS: SIEPEX, 2017. |
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Idioma: |
Português |
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Conteúdo: |
A macieira quando cultivada sob tela antigranizo exibe desenvolvimento vegetativo mais intenso do que observado em pomares a pleno sol. Ramos muito vigorosos prejudicam a eficiência de tratamentos fitossanitários e reduz a qualidade produtiva da planta. |
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Palavras-Chave: |
Macieira; Macieira Gala; Malus doméstica Borkh; Tela antigranizo. |
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Thesagro: |
Cultivo Protegido; Frutificação; Maçã; Malus doméstica; Pomar. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170258/1/2335-5378-1-PB-1.pdf
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Marc: |
LEADER 01130nam a2200265 a 4500
001 2084157
005 2018-01-03
008 2017 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, L. V.
245 $aManejo da poda verde em macieiras "Gala" em pomar sob tela antigranizo.$h[electronic resource]
260 $aIn: SALÃO INTEGRADO DE ENSINO, PESQUISA E EXTENSÃO, 7.; JORNADA DE PÓS-GRADUAÇÃO, 3.; SEMINÁRIO SOBRE TERRITORIALIDADE, 2., 2017, Tapes, RS. Anais... Tapes, RS: SIEPEX$c2017
520 $aA macieira quando cultivada sob tela antigranizo exibe desenvolvimento vegetativo mais intenso do que observado em pomares a pleno sol. Ramos muito vigorosos prejudicam a eficiência de tratamentos fitossanitários e reduz a qualidade produtiva da planta.
650 $aCultivo Protegido
650 $aFrutificação
650 $aMaçã
650 $aMalus doméstica
650 $aPomar
653 $aMacieira
653 $aMacieira Gala
653 $aMalus doméstica Borkh
653 $aTela antigranizo
700 1 $aGOULARTE, N. A. de A.
700 1 $aMAGRIN, F. P
700 1 $aHAWERROTH, F. J.
700 1 $aSIMOES, F.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/10/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SOUSA, P. C.; SANTOS, E. A. A.; SILVA, A. M.; BEZERRA, J. A. B.; SOUZA, A. L. P.; LIMA, G. L.; OLIVEIRA, M. F.; SILVA, A. R. |
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Afiliação: |
PATRÍCIA C. SOUSA, UFERSA; ERIKA A. A. SANTOS, UFERSA; ANDRÉIA M. SILVA, UFERSA; JOSÉ A. B. BEZERRA, UFERSA; ANA L. P. SOUZA, UFERSA; GABRIELA L. LIMA, UFRR; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA. |
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Título: |
Identification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 767-774, ago. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol. MenosThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented da... Mostrar Tudo |
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Palavras-Chave: |
Dano ultraestrutural e funcional; Euphractus sexcinctus; Membrane integrity; Mitochondrial activity; Six-banded armadillo; Sperm; Tatu-peba; Ultraestrutura; Ultrastructural and functional damages; Xenarthras. |
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Thesagro: |
Criopreservação; Espermatozoide; Membrana; Mitocondria; Sêmen. |
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Thesaurus NAL: |
Cryopreservation; Spermatozoa; Ultrastructure. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148299/1/Identification-of-ultrastructural.pdf
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Marc: |
LEADER 03089naa a2200421 a 4500
001 2054060
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSOUSA, P. C.
245 $aIdentification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation.
260 $c2016
520 $aThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol.
650 $aCryopreservation
650 $aSpermatozoa
650 $aUltrastructure
650 $aCriopreservação
650 $aEspermatozoide
650 $aMembrana
650 $aMitocondria
650 $aSêmen
653 $aDano ultraestrutural e funcional
653 $aEuphractus sexcinctus
653 $aMembrane integrity
653 $aMitochondrial activity
653 $aSix-banded armadillo
653 $aSperm
653 $aTatu-peba
653 $aUltraestrutura
653 $aUltrastructural and functional damages
653 $aXenarthras
700 1 $aSANTOS, E. A. A.
700 1 $aSILVA, A. M.
700 1 $aBEZERRA, J. A. B.
700 1 $aSOUZA, A. L. P.
700 1 $aLIMA, G. L.
700 1 $aOLIVEIRA, M. F.
700 1 $aSILVA, A. R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 767-774, ago. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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