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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
10/11/2017 |
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Data da última atualização: |
10/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIBEIRO, C. A. G.; PINTO, M. de O.; MACIEL, T. E. F.; PASTINA, M. M.; GOMES, E. G. de; GUIMARAES, C. T. |
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Afiliação: |
Carlos Alexandre Gomes Ribeiro, Universidade Federal de Viçosa; MARCOS DE OLIVEIRA PINTO, CNPMS; Talles Eduardo Ferreira Maciel, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; Everaldo Gonçalves de Barros, Universidade Católica de Brasília; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
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Título: |
Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientia Agricola, Piracicaba, v. 74, n. 2, p. 163-168, 2017. |
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DOI: |
10.1590/1678-992x-2016-0079 |
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Idioma: |
Inglês |
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Conteúdo: |
The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration. |
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Palavras-Chave: |
Caracterização genética; Genotipagem. |
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Thesagro: |
Variedade; Zea mays. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166544/1/Universal-tail.pdf
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Marc: |
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024 7 $a10.1590/1678-992x-2016-0079$2DOI
100 1 $aRIBEIRO, C. A. G.
245 $aUniversal tail sequence-SSR applied to molecular characterization of tropical maize hybrids.$h[electronic resource]
260 $c2017
520 $aThe development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.
650 $aVariedade
650 $aZea mays
653 $aCaracterização genética
653 $aGenotipagem
700 1 $aPINTO, M. de O.
700 1 $aMACIEL, T. E. F.
700 1 $aPASTINA, M. M.
700 1 $aGOMES, E. G. de
700 1 $aGUIMARAES, C. T.
773 $tScientia Agricola, Piracicaba$gv. 74, n. 2, p. 163-168, 2017.
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Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | RIBEIRO, C. A. G.; PINTO, M. de O.; MACIEL, T. E. F.; PASTINA, M. M.; GOMES, E. G. de; GUIMARAES, C. T. Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids. Scientia Agricola, Piracicaba, v. 74, n. 2, p. 163-168, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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