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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
25/10/2017 |
Data da última atualização: |
27/10/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, L. B. da; SILVA, L. L. da; FERREIRA, E. P. de B.; SANTOS, K. F. E. N.; MARTIN-DIDONET, C. C. G. |
Afiliação: |
LARISSA BATISTA DA SILVA, UNIVERSIDADE ESTADUAL DE GOIÁS; LUCAS LEONARDO DA SILVA, UNIVERSIDADE ESTADUAL DE GOIÁS; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF; KARINA FREIRE D'EÇA NOGUEIRA SANTOS, UNIVERSIDADE ESTADUAL DE GOIÁS; CLAUDIA CRISTINA GARCIA MARTIN-DIDONET, UNIVERSIDADE ESTADUAL DE GOIÁS. |
Título: |
Polyphasic characterization of endophytic bacteria of sorghum grown on cerrado soil of the Goiás State - Brazil. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
International Journal of Development Research, v. 7, n. 7, p. 14000-14007, July 2017. |
ISSN: |
2230-9926 |
Idioma: |
Inglês |
Conteúdo: |
Sorghum is a cereal of great commercial importance adapted to the tropical and subtropical regions. Isolation of plant-associated bacteria of agronomic interest has been the subject of several research works, however, there are few studies on bacteria isolating from sorghum. The objective of this work was to characterize as plant growth promoters endophytic bacteria isolated from sorghum roots. A total of 24 isolates were selected using the NFb-lactate, LGI-P, and YM semi-selective medium. The characterization was based on the resistance to antibiotics, use of carbon sources, enzyme activity and 16S-23S intergenic analysis. The isolates were more sensitive to the antibiotic streptomycin and showed a higher capacity to produce lipases and cellulases. Carbon sources of acid nature were more restrictive to the bacterial growth. Most of the isolates (95.8%) showed catalase and urease activity, besides to be able to solubilize phosphate. Intergenic analysis (16-23S rDNA) revealed a great diversity among the isolates, with eight groups with about 60% of similarity. In this study, it was possible to identify great physiological and genetic diversity among the bacteria isolated from sorghum roots. Some of these isolates can be considered promising plant growth-promoting rhizobacteria or sources of enzymes for biotechnological use. |
Palavras-Chave: |
Endophyticmicroorganisms; Extracellular enzymes; Phosphate solubilization; Sorghum. |
Thesagro: |
Bactéria; Cerrado; Solo; Sorgo. |
Thesaurus Nal: |
Phosphates. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165557/1/CNPAF-2017-ijdr.pdf
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Marc: |
LEADER 02228naa a2200289 a 4500 001 2078151 005 2017-10-27 008 2017 bl uuuu u00u1 u #d 022 $a2230-9926 100 1 $aSILVA, L. B. da 245 $aPolyphasic characterization of endophytic bacteria of sorghum grown on cerrado soil of the Goiás State - Brazil.$h[electronic resource] 260 $c2017 520 $aSorghum is a cereal of great commercial importance adapted to the tropical and subtropical regions. Isolation of plant-associated bacteria of agronomic interest has been the subject of several research works, however, there are few studies on bacteria isolating from sorghum. The objective of this work was to characterize as plant growth promoters endophytic bacteria isolated from sorghum roots. A total of 24 isolates were selected using the NFb-lactate, LGI-P, and YM semi-selective medium. The characterization was based on the resistance to antibiotics, use of carbon sources, enzyme activity and 16S-23S intergenic analysis. The isolates were more sensitive to the antibiotic streptomycin and showed a higher capacity to produce lipases and cellulases. Carbon sources of acid nature were more restrictive to the bacterial growth. Most of the isolates (95.8%) showed catalase and urease activity, besides to be able to solubilize phosphate. Intergenic analysis (16-23S rDNA) revealed a great diversity among the isolates, with eight groups with about 60% of similarity. In this study, it was possible to identify great physiological and genetic diversity among the bacteria isolated from sorghum roots. Some of these isolates can be considered promising plant growth-promoting rhizobacteria or sources of enzymes for biotechnological use. 650 $aPhosphates 650 $aBactéria 650 $aCerrado 650 $aSolo 650 $aSorgo 653 $aEndophyticmicroorganisms 653 $aExtracellular enzymes 653 $aPhosphate solubilization 653 $aSorghum 700 1 $aSILVA, L. L. da 700 1 $aFERREIRA, E. P. de B. 700 1 $aSANTOS, K. F. E. N. 700 1 $aMARTIN-DIDONET, C. C. G. 773 $tInternational Journal of Development Research$gv. 7, n. 7, p. 14000-14007, July 2017.
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