| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
26/09/2016 |
|
Data da última atualização: |
09/02/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ALVES, E. O. dos S.; LIMA NETO, F. P.; SANTOS, C. A. F.; RIBEIRO, I. C. N. dos S.; MELO, C. A. F. de; HOLANDA, I. S. A.; SOUZA, A. P. de; CORRÊA, R. X. |
|
Afiliação: |
ELAINI OLIVEIRA DOS SANTOS ALVES, Universidade Estadual de Santa Cruz, Ilhéus, BA; FRANCISCO PINHEIRO LIMA NETO, CPATSA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA; IERLA CARLA NUNES DOS SANTOS RIBEIRO; CLÁUSIO ANTÔNIO FERREIRA DE MELO, Universidade Estadual de Santa Cruz, Ilhéus, BA; IONÁ SANTOS ARAÚJO HOLANDA, UFERSA; ANETE PEREIRA DE SOUZA, UNICAMP; RONAN XAVIER CORRÊA, Universidade Estadual de Santa Cruz, Ilhéus, BA. |
|
Título: |
Genetic diversity of mango accessions (Mangifera indica) using new microsatellite markers and morphological descriptors. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Australian Journal of Crop Science, v. 10, n. 9, p. 1281-1287, 2016. |
|
ISSN: |
1835-2707 |
|
DOI: |
10.21475/ajcs.2016.10.09.p7729 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Genetic diversity estimates based on morphological and molecular data can provide different information on the relationship between cultivars of a species. This study aimed to develop new microsatellite markers as additional tools in genetic studies on mangoes (Mangifera indica L.), and to analyze the genetic variability of 20 mango cultivars based on morphological descriptors and microsatellite markers. We aimed to better understand the cultivars enhanced breeding histories and to support crossbreeding planning. Positive clones were selected from a DNA library enriched for microsatellite regions for sequencing and primer design. Four plants of each of the 20 accessions were used for observations, based on 48 morphological descriptors. Twenty accessions were analyzed using 27 microsatellite markers, of which 16 were developed during this study. The clusters, based on the morphological descriptors by Ward - MLM strategy and the microsatellite markers, suggested that Brazilian mango cultivars have extensive genetic diversity and are related to cultivars with different provenances, demonstrating their different enhanced breeding histories. |
|
Palavras-Chave: |
Diversidade genética; Genetic breeding; Mango; Melhoramento genético; Molecular marker; SSR; Variabilidade genética; Ward-MLM. |
|
Thesagro: |
Manga; Mangifera Indica; Marcador molecular. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147771/1/Carlos-2016.pdf
|
|
Marc: |
LEADER 02251naa a2200361 a 4500
001 2053435
005 2017-02-09
008 2016 bl uuuu u00u1 u #d
022 $a1835-2707
024 7 $a10.21475/ajcs.2016.10.09.p7729$2DOI
100 1 $aALVES, E. O. dos S.
245 $aGenetic diversity of mango accessions (Mangifera indica) using new microsatellite markers and morphological descriptors.$h[electronic resource]
260 $c2016
520 $aGenetic diversity estimates based on morphological and molecular data can provide different information on the relationship between cultivars of a species. This study aimed to develop new microsatellite markers as additional tools in genetic studies on mangoes (Mangifera indica L.), and to analyze the genetic variability of 20 mango cultivars based on morphological descriptors and microsatellite markers. We aimed to better understand the cultivars enhanced breeding histories and to support crossbreeding planning. Positive clones were selected from a DNA library enriched for microsatellite regions for sequencing and primer design. Four plants of each of the 20 accessions were used for observations, based on 48 morphological descriptors. Twenty accessions were analyzed using 27 microsatellite markers, of which 16 were developed during this study. The clusters, based on the morphological descriptors by Ward - MLM strategy and the microsatellite markers, suggested that Brazilian mango cultivars have extensive genetic diversity and are related to cultivars with different provenances, demonstrating their different enhanced breeding histories.
650 $aManga
650 $aMangifera Indica
650 $aMarcador molecular
653 $aDiversidade genética
653 $aGenetic breeding
653 $aMango
653 $aMelhoramento genético
653 $aMolecular marker
653 $aSSR
653 $aVariabilidade genética
653 $aWard-MLM
700 1 $aLIMA NETO, F. P.
700 1 $aSANTOS, C. A. F.
700 1 $aRIBEIRO, I. C. N. dos S.
700 1 $aMELO, C. A. F. de
700 1 $aHOLANDA, I. S. A.
700 1 $aSOUZA, A. P. de
700 1 $aCORRÊA, R. X.
773 $tAustralian Journal of Crop Science$gv. 10, n. 9, p. 1281-1287, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
19/10/2021 |
|
Data da última atualização: |
09/06/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
MERCANTE, L. A.; ANDRE, R. S.; FACURE, M. H. M.; FUGIKAWA-SANTOS, L.; CORREA, D. S. |
|
Afiliação: |
DANIEL SOUZA CORREA, CNPDIA. |
|
Título: |
Design of a bioelectronic tongue for glucose monitoring using zinc oxide nanofibers and graphene derivatives. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Sensors and Actuators Reports, v. 3, 100050, 2021. |
|
ISSN: |
2666-0539 |
|
DOI: |
https://doi.org/10.1016/j.snr.2021.100050 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Monitoring glucose levels is critical for diabetes management and might be a key step in the development of individualized treatment strategies. In this scenario, tracking salivary glucose has been recognized as a promising strategy due to its merits of ease sampling and non-invasive nature. In this paper, we report on the development of an electrical impedance-based biosensor array to distinguish glucose at different concentrations in saliva. The enzymatic biosensors were made of gold interdigitated electrodes coated with pristine electrospun zinc oxide nanofibers (NFZ) and NFZ combined with graphene-based nanomaterials (i.e., reduced graphene oxide - rGO and graphene quantum dots - GQDs), on which a layer of glucose oxidase (GOx) enzyme was adsorbed. Electrical impedance measurements indicate that the NFZ-GQDs@GOx and NFZ-rGO@GOx platforms presented good linear relationship with glucose concentration in the range of 0.1 to 6 mM. The highest sensitivity was reached for NFZ-rGO@GOx with a detection limit (LOD) of 14 μM, while the LOD was 32 μM for NFZ-GQDs@GOx. Both biosensors were also capable of detecting glucose in artificial saliva using aliquots of 10 μL, with recovery between 87.3 and 106.8%. Furthermore, the three sensing units (NFZ@GOx, NFZ-rGO@GOx and NFZ-GQDs@GOx) were employed to build a bioelectronic tongue. Using Principal Component Analysis (PCA) technique to project the electrical impedance data of all sensing units allowed the discrimination of the different glucose concentrations and interferents. This study reveals the applicability of the developed bioelectronic tongue as noninvasive glucose sensors, which approach could also be pottentially adapted to detect other disease biomarkers present in saliva MenosMonitoring glucose levels is critical for diabetes management and might be a key step in the development of individualized treatment strategies. In this scenario, tracking salivary glucose has been recognized as a promising strategy due to its merits of ease sampling and non-invasive nature. In this paper, we report on the development of an electrical impedance-based biosensor array to distinguish glucose at different concentrations in saliva. The enzymatic biosensors were made of gold interdigitated electrodes coated with pristine electrospun zinc oxide nanofibers (NFZ) and NFZ combined with graphene-based nanomaterials (i.e., reduced graphene oxide - rGO and graphene quantum dots - GQDs), on which a layer of glucose oxidase (GOx) enzyme was adsorbed. Electrical impedance measurements indicate that the NFZ-GQDs@GOx and NFZ-rGO@GOx platforms presented good linear relationship with glucose concentration in the range of 0.1 to 6 mM. The highest sensitivity was reached for NFZ-rGO@GOx with a detection limit (LOD) of 14 μM, while the LOD was 32 μM for NFZ-GQDs@GOx. Both biosensors were also capable of detecting glucose in artificial saliva using aliquots of 10 μL, with recovery between 87.3 and 106.8%. Furthermore, the three sensing units (NFZ@GOx, NFZ-rGO@GOx and NFZ-GQDs@GOx) were employed to build a bioelectronic tongue. Using Principal Component Analysis (PCA) technique to project the electrical impedance data of all sensing units allowed the discrimination of the different ... Mostrar Tudo |
|
Palavras-Chave: |
Electrospinning; Electrospinning Electrospun nanofibers; Reduced graphene oxide. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02503naa a2200229 a 4500
001 2135416
005 2022-06-09
008 2021 bl uuuu u00u1 u #d
022 $a2666-0539
024 7 $ahttps://doi.org/10.1016/j.snr.2021.100050$2DOI
100 1 $aMERCANTE, L. A.
245 $aDesign of a bioelectronic tongue for glucose monitoring using zinc oxide nanofibers and graphene derivatives.$h[electronic resource]
260 $c2021
520 $aMonitoring glucose levels is critical for diabetes management and might be a key step in the development of individualized treatment strategies. In this scenario, tracking salivary glucose has been recognized as a promising strategy due to its merits of ease sampling and non-invasive nature. In this paper, we report on the development of an electrical impedance-based biosensor array to distinguish glucose at different concentrations in saliva. The enzymatic biosensors were made of gold interdigitated electrodes coated with pristine electrospun zinc oxide nanofibers (NFZ) and NFZ combined with graphene-based nanomaterials (i.e., reduced graphene oxide - rGO and graphene quantum dots - GQDs), on which a layer of glucose oxidase (GOx) enzyme was adsorbed. Electrical impedance measurements indicate that the NFZ-GQDs@GOx and NFZ-rGO@GOx platforms presented good linear relationship with glucose concentration in the range of 0.1 to 6 mM. The highest sensitivity was reached for NFZ-rGO@GOx with a detection limit (LOD) of 14 μM, while the LOD was 32 μM for NFZ-GQDs@GOx. Both biosensors were also capable of detecting glucose in artificial saliva using aliquots of 10 μL, with recovery between 87.3 and 106.8%. Furthermore, the three sensing units (NFZ@GOx, NFZ-rGO@GOx and NFZ-GQDs@GOx) were employed to build a bioelectronic tongue. Using Principal Component Analysis (PCA) technique to project the electrical impedance data of all sensing units allowed the discrimination of the different glucose concentrations and interferents. This study reveals the applicability of the developed bioelectronic tongue as noninvasive glucose sensors, which approach could also be pottentially adapted to detect other disease biomarkers present in saliva
653 $aElectrospinning
653 $aElectrospinning Electrospun nanofibers
653 $aReduced graphene oxide
700 1 $aANDRE, R. S.
700 1 $aFACURE, M. H. M.
700 1 $aFUGIKAWA-SANTOS, L.
700 1 $aCORREA, D. S.
773 $tSensors and Actuators Reports$gv. 3, 100050, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
