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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
27/05/2016 |
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Data da última atualização: |
29/01/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TULLIO, L. D.; PAULITSCH, F.; REICHERT, P. R. S.; KLEPA, M. S.; KLEPA, M. S.; PILEGGI, M.; GOMES, D. F.; HUNGRIA, M.; BATISTA, J. S. S. |
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Afiliação: |
UEPG; UEPG; UEPG; UEPG; UEPG; MARIANGELA HUNGRIA DA CUNHA, CNPSO; UEPG. |
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Título: |
Proteomic analysis of Rhizobium freirei PRF 81 responses to low pH. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2015. |
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Idioma: |
Português |
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Notas: |
Res. 1407-2. |
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Conteúdo: |
Rhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions. MenosRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant chan... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143444/1/R1407-2.PDF
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Marc: |
LEADER 03060nam a2200241 a 4500
001 2045758
005 2018-01-29
008 2015 bl uuuu u00u1 u #d
100 1 $aTULLIO, L. D.
245 $aProteomic analysis of Rhizobium freirei PRF 81 responses to low pH.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia$c2015
500 $aRes. 1407-2.
520 $aRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aPAULITSCH, F.
700 1 $aREICHERT, P. R. S.
700 1 $aKLEPA, M. S.
700 1 $aKLEPA, M. S.
700 1 $aPILEGGI, M.
700 1 $aGOMES, D. F.
700 1 $aHUNGRIA, M.
700 1 $aBATISTA, J. S. S.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
28/12/2023 |
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Data da última atualização: |
28/12/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DAL PIZZOL, M. S.; IBELLI, A. M. G.; CANTAO, M. E.; PEIXOTO, J. de O.; FERNANDES, L. T.; MORES, M. A. Z.; CAMPOS, F. G.; OLIVEIRA, H. C. de; LEDUR, M. C. |
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Afiliação: |
MARIANE SPUDEIT DAL PIZZOL, Universidade do Estado de Santa Catarina/Campus Chapecó; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; LANA TEIXEIRA FERNANDES; MARCOS ANTONIO ZANELLA MORES, CNPSA; FRANCELLY GERALDA CAMPOS, Universidade Federal de Viçosa; HANIEL CEDRAZ DE OLIVEIRA, Universidade Federal de Viçosa; MONICA CORREA LEDUR, CNPSA. |
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Título: |
MicroRNAS da via de sinalização da insulina envolvidos na manifestação de white striping em frangos de corte aos 28 dias de idade. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: WORKSHOP DE CIÊNCIA, TECNOLOGIA E INOVAÇÃO, 7., 2023, Francisco Beltrão. Anais... Francisco Beltrão: UTFPR, 2023. p. 429-438. |
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Idioma: |
Português |
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Palavras-Chave: |
Epigenética; MicroRNAs; White Striping. |
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Thesagro: |
Frango de Corte. |
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Thesaurus NAL: |
Broiler chickens. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160310/1/final10167.pdf
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Marc: |
LEADER 00894nam a2200253 a 4500
001 2160310
005 2023-12-28
008 2023 bl uuuu u00u1 u #d
100 1 $aDAL PIZZOL, M. S.
245 $aMicroRNAS da via de sinalização da insulina envolvidos na manifestação de white striping em frangos de corte aos 28 dias de idade.$h[electronic resource]
260 $aIn: WORKSHOP DE CIÊNCIA, TECNOLOGIA E INOVAÇÃO, 7., 2023, Francisco Beltrão. Anais... Francisco Beltrão: UTFPR, 2023. p. 429-438.$c2023
650 $aBroiler chickens
650 $aFrango de Corte
653 $aEpigenética
653 $aMicroRNAs
653 $aWhite Striping
700 1 $aIBELLI, A. M. G.
700 1 $aCANTAO, M. E.
700 1 $aPEIXOTO, J. de O.
700 1 $aFERNANDES, L. T.
700 1 $aMORES, M. A. Z.
700 1 $aCAMPOS, F. G.
700 1 $aOLIVEIRA, H. C. de
700 1 $aLEDUR, M. C.
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