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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/01/2016 |
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Data da última atualização: |
02/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, A. D.; SCAPIM, C. A.; MACHADO, M. de F. P. da S.; MANGOLIN, C. A.; SILVA, T. A.; CANTAGALI, L. B.; TEIXEIRA, F. F.; MORA, F. |
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Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS. |
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Título: |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Scientia Agrícola, Piracicaba, v. 72, n. 6, p. 513-519, nov./dez. 2015. |
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DOI: |
http://dx.doi.org/10.1590/0103-9016-2014-0307 |
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Idioma: |
Português |
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Conteúdo: |
Information on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were recorded in the MG161-Branco Doce and Doce Cubano OPVs. The polymorphism information content ranged from 0.19 (Umc2319) to 0.71 (Umc2205). The analysis of molecular variance revealed that most of the genetic variability was concentrated within the cultivars of sweet corn (75 %), with less variability between them (25 %). The consensus tree derived from the neighbor-joining (NJ) algorithm using 1,000 bootstrapping replicates revealed seven genetically different groups. Nei?s diversity values varied between 0.103 (Doce do Hawai × CNPH-1 cultivars) and 0.645 (Amarelo Doce × Lili cultivars), indicating a narrow genetic basis. The Lili hybrid was the most distant cultivar, as revealed by Principal Coordinates Analysis and the NJ tree. This study on genetic diversity will be useful for planning future studies on sweet corn genetic resources and can complement the breeding programs for this crop. MenosInformation on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were recorded in the MG161-Branco Doce and Doce Cubano OPVs. The polymorphism information content ranged from 0.19 (Umc2319) to 0.71 (Umc2205). The analysis of molecular variance revealed that most of the genetic variability was concentrated within the cultivars of sweet corn (75 %), with less variability between them (25 %). The consensus tree derived from the neighbor-joining (NJ) algorithm using 1,000 bootstrapping replicates revealed seven genetically different groups. Nei?s diversity values varied between 0.103 (Doce do Hawai × CNPH-1 cultivars) and 0.645 (Amarelo Doce × Lili cultivars), indicating a narrow genetic basis. The Lili hybrid was the most distant cultivar, as revealed by Principal Coordinates Analysis and the N... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética. |
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Thesagro: |
Germoplasma; Milho. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137731/1/Genetic-diversity-1.pdf
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Marc: |
LEADER 02445naa a2200253 a 4500
001 2035081
005 2016-02-02
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/0103-9016-2014-0307$2DOI
100 1 $aLOPES, A. D.
245 $aGenetic diversity assessed by microsatellite markers in sweet corn cultivars.$h[electronic resource]
260 $c2015
520 $aInformation on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were recorded in the MG161-Branco Doce and Doce Cubano OPVs. The polymorphism information content ranged from 0.19 (Umc2319) to 0.71 (Umc2205). The analysis of molecular variance revealed that most of the genetic variability was concentrated within the cultivars of sweet corn (75 %), with less variability between them (25 %). The consensus tree derived from the neighbor-joining (NJ) algorithm using 1,000 bootstrapping replicates revealed seven genetically different groups. Nei?s diversity values varied between 0.103 (Doce do Hawai × CNPH-1 cultivars) and 0.645 (Amarelo Doce × Lili cultivars), indicating a narrow genetic basis. The Lili hybrid was the most distant cultivar, as revealed by Principal Coordinates Analysis and the NJ tree. This study on genetic diversity will be useful for planning future studies on sweet corn genetic resources and can complement the breeding programs for this crop.
650 $aGermoplasma
650 $aMilho
653 $aDiversidade genética
700 1 $aSCAPIM, C. A.
700 1 $aMACHADO, M. de F. P. da S.
700 1 $aMANGOLIN, C. A.
700 1 $aSILVA, T. A.
700 1 $aCANTAGALI, L. B.
700 1 $aTEIXEIRA, F. F.
700 1 $aMORA, F.
773 $tScientia Agrícola, Piracicaba$gv. 72, n. 6, p. 513-519, nov./dez. 2015.
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