Data/hora: 25/05/2024 14:10:31

Biblioteca(s): | Embrapa Agricultura Digital.
Data corrente: | 03/06/2009
Data da última atualização: | 30/11/2010
Autoria: | GAVIOLI, E. A.; DI MAURO, A. O.; CENTURION, M. A. P. da C.; DI MAURO, S. M. Z.
Afiliação: | EDUARDO ANTONIO GAVIOLI, ITES; ANTONIO ORLANDO DI MAURO, FCAV/UNESP; MARIA APARECIDA PESSOA DA CRUZ CENTURION, FCAV/UNESP; SÔNIA MARLI ZINGARETTI DI MAURO, UNESP.
Título: | Development of SCAR marker linked to stem canker resistance gene in soybean.
Ano de publicação: | 2007
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Londrina, v. 7, n. 2, p. 133-140, Mar. 2007.
Idioma: | Inglês
Conteúdo: | Stem canker caused by the fungus Diaporthe phaseolorum f. sp. meridionalis is a disease that limits soybean cultivation. Phenotypic evaluations aiming at disease resistance require labor-intensive processes, as for instance handling and transport of phytopathogens. The use of DNA markers in the selective procedures eases certain phases, besides being practical, safe and reliable. A RAPD fragment of 588pb was identified among bulks of resistant and susceptible plants in the cross BR92-15454 (R) x IAC-11 (S). Through co-segregation, the distance between the resistance locus and the fragment was estimated at 7.4 ± 2.1 cM, with a Lodmax. of 23.072 (first year) and at 6.0 ± 3.4 cM with a Lodmax. of 7.806 (second year). The fragment was converted into a SCAR marker and digested with enzyme Hinc II, which made the classification in homozygous resistant, heterozygous resistant and susceptible plants possible. This SCAR marker is suitable for use in the improvement program conducted in Jaboticabal.
Palavras-Chave: | Gene de resistência; RAPD; Resistência ao cancro da haste; Seleção assistida.
Thesagro: | Agricultura; Biotecnologia; Genética; Glycine Max; Soja.
Thesaurus Nal: | Diaporthe phaseolorum.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Agricultura Digital (CNPTIA)
Exemplares: | CNPTIA - (13083-1) - ADD - AP - PP -  -  -  - DIS