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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
14/10/2014 |
Data da última atualização: |
23/01/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ZULETA, L. F. G.; CUNHA, C. de O.; CARVALHO, F. M. de; CIAPINA, L. P.; SOUZA, R. C; MERCANTE, F. M.; FARIA, S. M. de; BALDANI, J. I.; STRALIOTTO, R.; HUNGRIA, M.; VASCONCELOS, A. T. R. de. |
Afiliação: |
SERGIO MIANA DE FARIA, CNPAB; JOSE IVO BALDANI, CNPAB; ROSANGELA STRALIOTTO, CNPAB. |
Título: |
The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
BMC Genomics, v.15, n. 1, p. 535, Jun. 2014. |
DOI: |
10.1186/1471-2164-15-535 |
Idioma: |
Português |
Notas: |
The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes |
Conteúdo: |
BACKGROUND: Burkholderia species play an important ecological role related to xenobiosis, the promotion of plant growth, the biocontrol of agricultural diseases, and symbiotic and non-symbiotic biological nitrogen fixation. Here, we highlight our study as providing the first complete genome of a symbiotic strain of B. phenoliruptrix, BR3459a (=CLA1), which was originally isolated in Brazil from nodules of Mimosa flocculosa and is effective in fixing nitrogen in association with this leguminous species. RESULTS: Genomic comparisons with other pathogenic and non-pathogenic Burkholderia strains grouped B. phenoliruptrix BR3459a with plant-associated beneficial and environmental species, although it shares a high percentage of its gene repertoire with species of the B. cepacia complex (Bcc) and "pseudomallei" group. The genomic analyses showed that the bce genes involved in exopolysaccharide production are clustered together in the same genomic region, constituting part of the Group III cluster of non-pathogenic bacteria. Regarding environmental stresses, we highlight genes that might be relevant in responses to osmotic, heat, cold and general stresses. Furthermore, a number of particularly interesting genes involved in the machinery of the T1SS, T2SS, T3SS, T4ASS and T6SS secretion systems were identified. The xenobiotic properties of strain BR3459a were also investigated, and some enzymes involved in the degradation of styrene, nitrotoluene, dioxin, chlorocyclohexane, chlorobenzene and caprolactam were identified. The genomic analyses also revealed a large number of antibiotic-related genes, the most important of which were correlated with streptomycin and novobiocin. The symbiotic plasmid showed high sequence identity with the symbiotic plasmid of B. phymatum. Additionally, comparative analysis of 545 housekeeping genes among pathogenic and non-pathogenic Burkholderia species strongly supports the definition of a new genus for the second branch, which would include BR3459a. CONCLUSIONS: The analyses of B. phenoliruptrix BR3459a showed key property of fixing nitrogen that together with genes for high tolerance to environmental stresses might explain a successful strategy of symbiosis in the tropics. The strain also harbours interesting sets of genes with biotechnological potential. However, the resemblance of certain genes to those of pathogenic Burkholderia raise concerns about large-scale applications in agriculture or for bioremediation. MenosBACKGROUND: Burkholderia species play an important ecological role related to xenobiosis, the promotion of plant growth, the biocontrol of agricultural diseases, and symbiotic and non-symbiotic biological nitrogen fixation. Here, we highlight our study as providing the first complete genome of a symbiotic strain of B. phenoliruptrix, BR3459a (=CLA1), which was originally isolated in Brazil from nodules of Mimosa flocculosa and is effective in fixing nitrogen in association with this leguminous species. RESULTS: Genomic comparisons with other pathogenic and non-pathogenic Burkholderia strains grouped B. phenoliruptrix BR3459a with plant-associated beneficial and environmental species, although it shares a high percentage of its gene repertoire with species of the B. cepacia complex (Bcc) and "pseudomallei" group. The genomic analyses showed that the bce genes involved in exopolysaccharide production are clustered together in the same genomic region, constituting part of the Group III cluster of non-pathogenic bacteria. Regarding environmental stresses, we highlight genes that might be relevant in responses to osmotic, heat, cold and general stresses. Furthermore, a number of particularly interesting genes involved in the machinery of the T1SS, T2SS, T3SS, T4ASS and T6SS secretion systems were identified. The xenobiotic properties of strain BR3459a were also investigated, and some enzymes involved in the degradation of styrene, nitrotoluene, dioxin, chlorocyclohexane, chlorobe... Mostrar Tudo |
Palavras-Chave: |
Comparative genomics; FBN; Genômica; Nitrogen fixing; Pathogenic Bacteria. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03613naa a2200325 a 4500 001 1997358 005 2015-01-23 008 2014 bl --- 0-- u #d 024 7 $a10.1186/1471-2164-15-535$2DOI 100 1 $aZULETA, L. F. G. 245 $aThe complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa$bhighlighting the coexistence of symbiotic and pathogenic genes. 260 $c2014 500 $aThe complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes 520 $aBACKGROUND: Burkholderia species play an important ecological role related to xenobiosis, the promotion of plant growth, the biocontrol of agricultural diseases, and symbiotic and non-symbiotic biological nitrogen fixation. Here, we highlight our study as providing the first complete genome of a symbiotic strain of B. phenoliruptrix, BR3459a (=CLA1), which was originally isolated in Brazil from nodules of Mimosa flocculosa and is effective in fixing nitrogen in association with this leguminous species. RESULTS: Genomic comparisons with other pathogenic and non-pathogenic Burkholderia strains grouped B. phenoliruptrix BR3459a with plant-associated beneficial and environmental species, although it shares a high percentage of its gene repertoire with species of the B. cepacia complex (Bcc) and "pseudomallei" group. The genomic analyses showed that the bce genes involved in exopolysaccharide production are clustered together in the same genomic region, constituting part of the Group III cluster of non-pathogenic bacteria. Regarding environmental stresses, we highlight genes that might be relevant in responses to osmotic, heat, cold and general stresses. Furthermore, a number of particularly interesting genes involved in the machinery of the T1SS, T2SS, T3SS, T4ASS and T6SS secretion systems were identified. The xenobiotic properties of strain BR3459a were also investigated, and some enzymes involved in the degradation of styrene, nitrotoluene, dioxin, chlorocyclohexane, chlorobenzene and caprolactam were identified. The genomic analyses also revealed a large number of antibiotic-related genes, the most important of which were correlated with streptomycin and novobiocin. The symbiotic plasmid showed high sequence identity with the symbiotic plasmid of B. phymatum. Additionally, comparative analysis of 545 housekeeping genes among pathogenic and non-pathogenic Burkholderia species strongly supports the definition of a new genus for the second branch, which would include BR3459a. CONCLUSIONS: The analyses of B. phenoliruptrix BR3459a showed key property of fixing nitrogen that together with genes for high tolerance to environmental stresses might explain a successful strategy of symbiosis in the tropics. The strain also harbours interesting sets of genes with biotechnological potential. However, the resemblance of certain genes to those of pathogenic Burkholderia raise concerns about large-scale applications in agriculture or for bioremediation. 653 $aComparative genomics 653 $aFBN 653 $aGenômica 653 $aNitrogen fixing 653 $aPathogenic Bacteria 700 1 $aCUNHA, C. de O. 700 1 $aCARVALHO, F. M. de 700 1 $aCIAPINA, L. P. 700 1 $aSOUZA, R. C 700 1 $aMERCANTE, F. M. 700 1 $aFARIA, S. M. de 700 1 $aBALDANI, J. I. 700 1 $aSTRALIOTTO, R. 700 1 $aHUNGRIA, M. 700 1 $aVASCONCELOS, A. T. R. de 773 $tBMC Genomics$gv.15, n. 1, p. 535, Jun. 2014.
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Embrapa Agrobiologia (CNPAB) |
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Registros recuperados : 5 | |
1. | | ZULETA, L. F. G.; CUNHA, C. de O.; CARVALHO, F. M. de; CIAPINA, L. P.; SOUZA, R. C; MERCANTE, F. M.; FARIA, S. M. de; BALDANI, J. I.; STRALIOTTO, R.; HUNGRIA, M.; VASCONCELOS, A. T. R. de. The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes. BMC Genomics, v.15, p. 535, Jun. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Soja. |
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2. | | ZULETA, L. F. G.; CUNHA, C. de O.; CARVALHO, F. M. de; CIAPINA, L. P.; SOUZA, R. C; MERCANTE, F. M.; FARIA, S. M. de; BALDANI, J. I.; STRALIOTTO, R.; HUNGRIA, M.; VASCONCELOS, A. T. R. de. The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes. BMC Genomics, v.15, n. 1, p. 535, Jun. 2014. The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genesTipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agrobiologia. |
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3. | | CUNHA, C. de O.; ZULETA, L. F. G.; ALMEIDA, L. G. P. de; CIAPINA, L. P.; BORGES, W. L.; PITARD, R. M.; BALDANI, J. I.; STRALIOTTO, R.; FARIA, S. M. de; HUNGRIA, M. Complete genome sequence of Burkholderia phenoliruptriz BR3459a (CLA 1), a heat-tolerant, nitrogen fixing symbiont of Mimosa flocculosa. Journal of bacteriology, v. 194, n. 23, p. 6675-6676, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agrobiologia. |
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4. | | CUNHA, C. de O. C.; ZULETA, L. F. G.; ALMEIDA, L. G. P. de; CIAPINA, L. P.; BORGES, W. L.; PITARD, R. M.; BALDANI, J. I.; STRALIOTTO, R.; FARIA, S. M. de; HUNGRIA, M.; CAVADA, B. S.; MERCANTE, F. M.; VASCONCELOS, A. T. R. de. Complete genome sequence of Burkholderia phenoliruptrix BR3459a (CLA1), a heat-tolerant, nitrogen-fixing symbiont of Mimosa flocculosa. Journal of Bacteriology, Washington, v. 194, n. 23, p. 6675-6676, Dec. 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agrobiologia; Embrapa Soja. |
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5. | | CUNHA, C. de O.; ZULETA, L. F. G.; ALMEIDA, L. G. P. de; CIAPINA, L. P.; BORGES, W. L.; PITARD, R. M.; BALDANI, J. I.; STRALIOTTO, R.; FARIA, S. M. de; HUNGRIA, M.; CAVADA, B. S.; MERCANTE, F. M.; VASCONCELOS, A. T. R. de. Complete genome sequence of Burkholderia phenoliruptrix BR3459a (CLA1), a heat-tolerant, nitrogen-fixing symbiont of mimosa flocculosa. Journal of Bacteriology, Washington, v. 194, n. 23, p. 6675-6676, Dec. 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Amapá. |
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Registros recuperados : 5 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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