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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
08/09/2023 |
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Data da última atualização: |
08/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. de; SILVA, C. B. da; HELM, C. V.; ZANONI, P. R. S.; AUER, C. G.; MIGUEL, M. D. |
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Afiliação: |
MAISLIAN DE OLIVEIRA, UNIVERSIDADE FEDERAL DO PARANÁ; CRISTIANE BEZERRA DA SILVA, UNIVERSIDADE FEDERAL DO PARANÁ; CRISTIANE VIEIRA HELM, CNPF; PATRICIA RAQUEL SILVA ZANONI, CNPF; CELSO GARCIA AUER, CNPF; MARILIS DALLARMI MIGUEL, UNIVERSIDADE FEDERAL DO PARANÁ. |
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Título: |
Al2(SO4)3 alters the antioxidant mitochondrial metabolism of Botritys cinerea and optimizes the production of cellulose and oxidative degrading enzymes. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Research, Society and Development, v. 12, n. 8, e14412842996, 12 p., 2023. |
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Idioma: |
Português |
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Conteúdo: |
Abstract The enzymes produced by pathogenic fungi, especially Botritys cinerea, deserve specific attention due to the diversity of their applications, mainly in biofuel production, food processing, and the pharmaceutical industry. Thus, this work used Al2(SO4)3 as a stressor in order to evaluate if the stress levels caused by the concentrations of 100, 250, 500, and 1000 ppm were sufficient to increase the production of hydrolytic cellulolytic enzymes (FPase, CMCase, Avicelase, β- glucosidase, xylanase) and oxidative (laccase and manganese peroxidase). The study also evaluated the stress levels in previously treated mycelia of B. cinerea and whether they corresponded to the different states of mitochondrial respiration. Our study indicates that Al2(SO4)3 increased the production of cellulolytic and oxidative enzymes in all concentrations in a dose-dependent manner and that Al2(SO4)3 alters the mitochondrial respiratory rate, with lower ATP productions, indicating that less-coupled mitochondria were obtained and that this may be due to the increase of oxidative stress. Thus, it is plausible to suggest the use of Al2(SO4)3 in the production of cellulolytic enzymes, which could be used in the hydrolysis stage of second-generation ethanol production processes, as it reduces the time required for enzymatic expression applications in industrial processes. Resumo As enzimas produzidas por fungos patogênicos, especialmente Botritys cinerea, merecem atenção especial devido à diversidade de suas aplicações, principalmente na produção de biocombustíveis, processamento de alimentos e indústria farmacêutica. Assim, este trabalho utilizou o Al2(SO4)3 como estressor a fim de avaliar se os níveis de estresse causados pelas concentrações de 100, 250, 500 e 1000 ppm foram suficientes para aumentar a produção de enzimas celulolíticas hidrolíticas (FPase, CMCase, avicelase, β-glicosidase, xilanase) e oxidativo (lacase e manganês peroxidase). O estudo também avaliou os níveis de estresse em micélios de B. cinerea previamente tratados e se eles correspondiam aos diferentes estados da respiração mitocondrial. Nosso estudo indica que Al2(SO4)3 aumentou a produção de enzimas celulolíticas e oxidativas em todas as concentrações de forma dose-dependente e que Al2(SO4)3 altera a taxa respiratória mitocondrial, com menor produção de ATP, indicando que foram obtidas mitocôndrias menos acopladas e que isso pode ser devido ao aumento do estresse oxidativo. Assim, é plausível sugerir o uso de Al2(SO4)3 na produção de enzimas celulolíticas, poderia ser utilizado na etapa de hidrólise de processos de produção de etanol de segunda geração, pois reduz o tempo necessário para aplicações de expressão enzimática em processos industriais. Palavras-chave: Estresse oxidativo; Acoplamento mitocondrial; Catalase; Peroxidase; Botritys cinerea. MenosAbstract The enzymes produced by pathogenic fungi, especially Botritys cinerea, deserve specific attention due to the diversity of their applications, mainly in biofuel production, food processing, and the pharmaceutical industry. Thus, this work used Al2(SO4)3 as a stressor in order to evaluate if the stress levels caused by the concentrations of 100, 250, 500, and 1000 ppm were sufficient to increase the production of hydrolytic cellulolytic enzymes (FPase, CMCase, Avicelase, β- glucosidase, xylanase) and oxidative (laccase and manganese peroxidase). The study also evaluated the stress levels in previously treated mycelia of B. cinerea and whether they corresponded to the different states of mitochondrial respiration. Our study indicates that Al2(SO4)3 increased the production of cellulolytic and oxidative enzymes in all concentrations in a dose-dependent manner and that Al2(SO4)3 alters the mitochondrial respiratory rate, with lower ATP productions, indicating that less-coupled mitochondria were obtained and that this may be due to the increase of oxidative stress. Thus, it is plausible to suggest the use of Al2(SO4)3 in the production of cellulolytic enzymes, which could be used in the hydrolysis stage of second-generation ethanol production processes, as it reduces the time required for enzymatic expression applications in industrial processes. Resumo As enzimas produzidas por fungos patogênicos, especialmente Botritys cinerea, merecem atenção especial devido à div... Mostrar Tudo |
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Palavras-Chave: |
Acoplamento mitocondrial; Botritys cinerea; Estresse oxidativo; Mitochondrial coupling. |
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Thesagro: |
Catalase; Peroxidase. |
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Thesaurus Nal: |
Oxidative stress; Peroxidases. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156531/1/ResearchSocietyDevelopment-2023-BotritysCinerea.pdf
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Marc: |
LEADER 03794naa a2200277 a 4500
001 2156531
005 2023-09-08
008 2023 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, M. de
245 $aAl2(SO4)3 alters the antioxidant mitochondrial metabolism of Botritys cinerea and optimizes the production of cellulose and oxidative degrading enzymes.$h[electronic resource]
260 $c2023
520 $aAbstract The enzymes produced by pathogenic fungi, especially Botritys cinerea, deserve specific attention due to the diversity of their applications, mainly in biofuel production, food processing, and the pharmaceutical industry. Thus, this work used Al2(SO4)3 as a stressor in order to evaluate if the stress levels caused by the concentrations of 100, 250, 500, and 1000 ppm were sufficient to increase the production of hydrolytic cellulolytic enzymes (FPase, CMCase, Avicelase, β- glucosidase, xylanase) and oxidative (laccase and manganese peroxidase). The study also evaluated the stress levels in previously treated mycelia of B. cinerea and whether they corresponded to the different states of mitochondrial respiration. Our study indicates that Al2(SO4)3 increased the production of cellulolytic and oxidative enzymes in all concentrations in a dose-dependent manner and that Al2(SO4)3 alters the mitochondrial respiratory rate, with lower ATP productions, indicating that less-coupled mitochondria were obtained and that this may be due to the increase of oxidative stress. Thus, it is plausible to suggest the use of Al2(SO4)3 in the production of cellulolytic enzymes, which could be used in the hydrolysis stage of second-generation ethanol production processes, as it reduces the time required for enzymatic expression applications in industrial processes. Resumo As enzimas produzidas por fungos patogênicos, especialmente Botritys cinerea, merecem atenção especial devido à diversidade de suas aplicações, principalmente na produção de biocombustíveis, processamento de alimentos e indústria farmacêutica. Assim, este trabalho utilizou o Al2(SO4)3 como estressor a fim de avaliar se os níveis de estresse causados pelas concentrações de 100, 250, 500 e 1000 ppm foram suficientes para aumentar a produção de enzimas celulolíticas hidrolíticas (FPase, CMCase, avicelase, β-glicosidase, xilanase) e oxidativo (lacase e manganês peroxidase). O estudo também avaliou os níveis de estresse em micélios de B. cinerea previamente tratados e se eles correspondiam aos diferentes estados da respiração mitocondrial. Nosso estudo indica que Al2(SO4)3 aumentou a produção de enzimas celulolíticas e oxidativas em todas as concentrações de forma dose-dependente e que Al2(SO4)3 altera a taxa respiratória mitocondrial, com menor produção de ATP, indicando que foram obtidas mitocôndrias menos acopladas e que isso pode ser devido ao aumento do estresse oxidativo. Assim, é plausível sugerir o uso de Al2(SO4)3 na produção de enzimas celulolíticas, poderia ser utilizado na etapa de hidrólise de processos de produção de etanol de segunda geração, pois reduz o tempo necessário para aplicações de expressão enzimática em processos industriais. Palavras-chave: Estresse oxidativo; Acoplamento mitocondrial; Catalase; Peroxidase; Botritys cinerea.
650 $aOxidative stress
650 $aPeroxidases
650 $aCatalase
650 $aPeroxidase
653 $aAcoplamento mitocondrial
653 $aBotritys cinerea
653 $aEstresse oxidativo
653 $aMitochondrial coupling
700 1 $aSILVA, C. B. da
700 1 $aHELM, C. V.
700 1 $aZANONI, P. R. S.
700 1 $aAUER, C. G.
700 1 $aMIGUEL, M. D.
773 $tResearch, Society and Development$gv. 12, n. 8, e14412842996, 12 p., 2023.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
06/01/2020 |
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Data da última atualização: |
07/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PAIVA, A. L. B.; MUDADU, M. de A.; PEREIRA, E. H. T.; MARRI, C. A.; GUERRA-DUARTE, C.; DINIZ, M. R. V. |
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Afiliação: |
ANA L. B. PAIVA, Fundação Ezequiel Dias; MAURICIO DE ALVARENGA MUDADU, CNPTIA; ELAINE H. T. PEREIRA; CAMILA A. MARRI; CLARA GUERRA-DUARTE; MARCELO R. V. DINIZ. |
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Título: |
Transcriptome analysis of the spider Phoneutria pertyi venom glands reveals novel venom components for the genus Phoneutria. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Toxicon, v. 163, p. 59-69, May 2019. |
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DOI: |
https://doi.org/10.1016/j.toxicon.2019.03.014 |
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Idioma: |
Inglês |
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Conteúdo: |
Phoneutria nigriventer spider venom has been studied for more than 40 years and several components with pharmacological potential have been described in it. However, studies on venoms from other species of the Phoneutria genus are scarce. In this work, a conventional cDNA library from the species Phoneutria pertyi venom glands was constructed, aiming to identify novel putative cysteine-rich peptide toxins for the genus Phoneutria. 296 unique sequences were identified and 51 sequences corresponded to putative cysteine-rich peptide toxins. Besides cysteine-rich peptide toxins, other putative venom components such as protease inhibitors, defensins and serine proteinases were identified. Furthermore, by manual curation of the sequences with no match at UniProt, we were able to identify glycine-rich proteins (GRP), a class of venom component never described in Phoneutria genus. This work describes the first complete sequences of toxins from the venom of P. pertyi and reveals that, despite most of the retrieved toxins show a high identity to toxins identified in Phoneutria genus, novel putative toxins remains to be described. |
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Palavras-Chave: |
Cysteine-rich toxin; Toxina rica em cisteína; Transcriptoma; Veneno de aranha; Venom glands. |
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Thesagro: |
Aranha. |
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Thesaurus NAL: |
Phoneutria nigriventer; Transcriptome. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02036naa a2200289 a 4500
001 2118189
005 2020-01-07
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.toxicon.2019.03.014$2DOI
100 1 $aPAIVA, A. L. B.
245 $aTranscriptome analysis of the spider Phoneutria pertyi venom glands reveals novel venom components for the genus Phoneutria.$h[electronic resource]
260 $c2019
520 $aPhoneutria nigriventer spider venom has been studied for more than 40 years and several components with pharmacological potential have been described in it. However, studies on venoms from other species of the Phoneutria genus are scarce. In this work, a conventional cDNA library from the species Phoneutria pertyi venom glands was constructed, aiming to identify novel putative cysteine-rich peptide toxins for the genus Phoneutria. 296 unique sequences were identified and 51 sequences corresponded to putative cysteine-rich peptide toxins. Besides cysteine-rich peptide toxins, other putative venom components such as protease inhibitors, defensins and serine proteinases were identified. Furthermore, by manual curation of the sequences with no match at UniProt, we were able to identify glycine-rich proteins (GRP), a class of venom component never described in Phoneutria genus. This work describes the first complete sequences of toxins from the venom of P. pertyi and reveals that, despite most of the retrieved toxins show a high identity to toxins identified in Phoneutria genus, novel putative toxins remains to be described.
650 $aPhoneutria nigriventer
650 $aTranscriptome
650 $aAranha
653 $aCysteine-rich toxin
653 $aToxina rica em cisteína
653 $aTranscriptoma
653 $aVeneno de aranha
653 $aVenom glands
700 1 $aMUDADU, M. de A.
700 1 $aPEREIRA, E. H. T.
700 1 $aMARRI, C. A.
700 1 $aGUERRA-DUARTE, C.
700 1 $aDINIZ, M. R. V.
773 $tToxicon$gv. 163, p. 59-69, May 2019.
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Embrapa Agricultura Digital (CNPTIA) |
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