| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agricultura Digital. |
|
Data corrente: |
09/01/2020 |
|
Data da última atualização: |
04/02/2020 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. |
|
Afiliação: |
LAUDIANE ZANELLA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JOÃO CARLOS BESPALHOK FILHO, UFPR. |
|
Título: |
RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. |
|
Idioma: |
Inglês |
|
Notas: |
Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. |
|
Conteúdo: |
From the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants. MenosFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histoch... Mostrar Tudo |
|
Thesaurus Nal: |
Populus alba; Populus tremula. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02291nam a2200181 a 4500
001 2118433
005 2020-02-04
008 2019 bl uuuu u01u1 u #d
100 1 $aZANELLA, L.
245 $aRNA interference supression of DOF transcription factor from Populus tremula x Populus alba.$h[electronic resource]
260 $aPesquisa Florestal Brasileira, v. 39, e201902043, p. 322$c2019
500 $aNa publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.
520 $aFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants.
650 $aPopulus alba
650 $aPopulus tremula
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aGERHARDT, I. R.
700 1 $aBESPALHOK FILHO, J. C.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
11/07/1994 |
|
Data da última atualização: |
03/05/2000 |
|
Autoria: |
NASCIMENTO, W. M.; ANDREOLI, C. |
|
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
|
Título: |
Controle de qualidade no beneficiamento de sementes de cenoura. |
|
Ano de publicação: |
1990 |
|
Fonte/Imprenta: |
Revista Brasileira de Sementes, Brasilia, v.12, n.2, p.28-36, 1990. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Carrot; Conditioning; Cultivar Kuronan; Kuronan; Processing; Quality; Seed. |
|
Thesagro: |
Beneficiamento; Cenoura; Cerrado; Controle de Qualidade; Daucus Carota; Germinação; Qualidade; Semente. |
|
Thesaurus NAL: |
carrots; germination; quality control; seeds; varieties. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00909naa a2200361 a 4500
001 1768521
005 2000-05-03
008 1990 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, W. M.
245 $aControle de qualidade no beneficiamento de sementes de cenoura.
260 $c1990
650 $acarrots
650 $agermination
650 $aquality control
650 $aseeds
650 $avarieties
650 $aBeneficiamento
650 $aCenoura
650 $aCerrado
650 $aControle de Qualidade
650 $aDaucus Carota
650 $aGerminação
650 $aQualidade
650 $aSemente
653 $aCarrot
653 $aConditioning
653 $aCultivar Kuronan
653 $aKuronan
653 $aProcessing
653 $aQuality
653 $aSeed
700 1 $aANDREOLI, C.
773 $tRevista Brasileira de Sementes, Brasilia$gv.12, n.2, p.28-36, 1990.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Hortaliças (CNPH) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
