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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/01/2023 |
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Data da última atualização: |
09/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASTRO, I. S. L.; BARREIROS, P. R. R. M.; MENDES, T. A. de O.; FLOREZ, J. C.; SILVA, E. M. de A.; PORTO, B. N.; ZAMBOLIM, L.; CAIXETA, E. T. |
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Afiliação: |
ISABEL SAMILA LIMA CASTRO, UNIVERSIDADE FEDERAL DE VIÇOSA; PEDRO RICARDO ROSSI MARQUES BARREIROS, UNIVERSIDADE FEDERAL DE VIÇOSA; TIAGO ANTÔNIO DE OLIVEIRA MENDES, UNIVERSIDADE FEDERAL DE VIÇOSA; JUAN CARLOS FLOREZ, UNIVERSIDADE FEDERAL DE VIÇOSA; EDSON MARIO DE ANDRADE SILVA, UNIVERSIDADE FEDERAL DE VIÇOSA; BRENDA NEVES PORTO, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa. |
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Título: |
Gene expression and interactome analysis of candidate effectors associated with pre- and post-haustorial hemileia vastatrix-coffee interaction. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Biotechnology and Biomedicine, v. 5, n. 4, p. 198-213, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
The present study sought to analyze the putative secreted proteins of Hemileia vastatrix, with potential to function as effector proteins. The H. vastatrix secretome was subjected to functional categorization and the greatest similarities were observed between species of the genus Puccinia sp (398), and Melampsora larici-populina (82). Based on the secretome, 415 Gene Ontology terms were extracted. The putative secretome was also compared to the high-throughput transcriptome of coffee-H. vastatrix interactions. By the transcriptome comparison data and the results of functional annotation and characteristics associated with effector proteins, 15 genes were selected and analyzed using RT-qPCR during compatible and incompatible coffee-H. vastatrix interactions. The expression patterns suggested that the EHv33-18 and EHv33-25 candidate effector may be responsible for faster communication between pathogen and the host during incompatible interaction. Other six candidate are involved in the biotrophic stage of infection, which is characterized by an increase in the expression of effectors, and in enzymes involved in secondary metabolism. Phylogenetic analysis suggest that these eight genes follow evolutionary mechanisms exclusive to the coffee-H. vastatrix interaction, making them important targets in studies aimed at obtaining durable resistance to this disease. Interactomic network made between coffee proteins and H. vastatrix proteins was obtained for the first time and revealed a wide network of interactions between effector EHv33-19 and coffee proteins. The obtained results suggest that there may be communication between the pathogen and the host in the early stage of infection during the urediniospores germination phase. This indicated pre-haustorial resistance complementary to post-haustorial resistance. MenosThe present study sought to analyze the putative secreted proteins of Hemileia vastatrix, with potential to function as effector proteins. The H. vastatrix secretome was subjected to functional categorization and the greatest similarities were observed between species of the genus Puccinia sp (398), and Melampsora larici-populina (82). Based on the secretome, 415 Gene Ontology terms were extracted. The putative secretome was also compared to the high-throughput transcriptome of coffee-H. vastatrix interactions. By the transcriptome comparison data and the results of functional annotation and characteristics associated with effector proteins, 15 genes were selected and analyzed using RT-qPCR during compatible and incompatible coffee-H. vastatrix interactions. The expression patterns suggested that the EHv33-18 and EHv33-25 candidate effector may be responsible for faster communication between pathogen and the host during incompatible interaction. Other six candidate are involved in the biotrophic stage of infection, which is characterized by an increase in the expression of effectors, and in enzymes involved in secondary metabolism. Phylogenetic analysis suggest that these eight genes follow evolutionary mechanisms exclusive to the coffee-H. vastatrix interaction, making them important targets in studies aimed at obtaining durable resistance to this disease. Interactomic network made between coffee proteins and H. vastatrix proteins was obtained for the first time and reveale... Mostrar Tudo |
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Thesaurus Nal: |
Gene interaction; Hemileia; Phylogeny; Plant pathogens. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150799/1/gene-expression-and-interactome.pdf
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Marc: |
LEADER 02645naa a2200253 a 4500
001 2150799
005 2023-01-09
008 2022 bl uuuu u00u1 u #d
100 1 $aCASTRO, I. S. L.
245 $aGene expression and interactome analysis of candidate effectors associated with pre- and post-haustorial hemileia vastatrix-coffee interaction.$h[electronic resource]
260 $c2022
520 $aThe present study sought to analyze the putative secreted proteins of Hemileia vastatrix, with potential to function as effector proteins. The H. vastatrix secretome was subjected to functional categorization and the greatest similarities were observed between species of the genus Puccinia sp (398), and Melampsora larici-populina (82). Based on the secretome, 415 Gene Ontology terms were extracted. The putative secretome was also compared to the high-throughput transcriptome of coffee-H. vastatrix interactions. By the transcriptome comparison data and the results of functional annotation and characteristics associated with effector proteins, 15 genes were selected and analyzed using RT-qPCR during compatible and incompatible coffee-H. vastatrix interactions. The expression patterns suggested that the EHv33-18 and EHv33-25 candidate effector may be responsible for faster communication between pathogen and the host during incompatible interaction. Other six candidate are involved in the biotrophic stage of infection, which is characterized by an increase in the expression of effectors, and in enzymes involved in secondary metabolism. Phylogenetic analysis suggest that these eight genes follow evolutionary mechanisms exclusive to the coffee-H. vastatrix interaction, making them important targets in studies aimed at obtaining durable resistance to this disease. Interactomic network made between coffee proteins and H. vastatrix proteins was obtained for the first time and revealed a wide network of interactions between effector EHv33-19 and coffee proteins. The obtained results suggest that there may be communication between the pathogen and the host in the early stage of infection during the urediniospores germination phase. This indicated pre-haustorial resistance complementary to post-haustorial resistance.
650 $aGene interaction
650 $aHemileia
650 $aPhylogeny
650 $aPlant pathogens
700 1 $aBARREIROS, P. R. R. M.
700 1 $aMENDES, T. A. de O.
700 1 $aFLOREZ, J. C.
700 1 $aSILVA, E. M. de A.
700 1 $aPORTO, B. N.
700 1 $aZAMBOLIM, L.
700 1 $aCAIXETA, E. T.
773 $tJournal of Biotechnology and Biomedicine$gv. 5, n. 4, p. 198-213, 2022.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 207 | |
| 18. |  | SANTANA, M. F.; ZAMBOLIM, E. M.; OLIVEIRA, L. O. de; CAIXETA, E. T.; ZAMBOLIM, L. Análise molecular do rDNA de Hemileia vastatrix. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 19. | | COSTA, R. V.; ZAMBOLIM, L.; SANTOS, F. G.; VALE, F. X. R.; CASELA, C. R. Avaliação da diversificação genética da produção de hospedeira no manejo da antracnose do sorgo. Fitopatologia Brasileira, Brasília, DF, v. 31, p. S205-S206, ago. 2006. Edição dos Resumos do XXXIX Congresso Brasileiro de Fitopatologia, Salvador, BA, ago. 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 20. | | ALVARENGA, S. M.; CAIXETA, E. T.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; SAKIYAMA, N. S. Caracterização funcional e perfil de expressão in silico de quitinases do CAFEST. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 207 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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