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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/01/2001 |
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Data da última atualização: |
18/01/2001 |
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Autoria: |
SWIDERSKI, M. R.; ZABOROWSKA, Z.; LEGOCKI, A. B. |
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Título: |
Identification of new nodulin cDNAs from yellow lupine by differential display. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Plant Science, Ireland, v. 151, p. 75-83, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
In this study several sequences that represent yellow lupine nodulin genes are identified. Four different cDNA clones were isolated from a lupine nodule cDNA library and sequenced. Database search revealed that the deduced amino acid of one clone, DD3A20, shows near 80% amino acid similarity to plant stearoyl-acyl carrier protein desaturases and contains crucial amino acids in the fatty acid binding domain which are characteristic for stearoyl-ACP desaturases. The other sequences seem to be unique. Nearly identical signal peptide sequences are present at the N-termini of the DD4A9 and DD2T15 putative poteins. Northern analysis indicated that the pattern of expression of the Lldd4A9, Lldd2A18 and Lldd2T15 genes is the same as that of the lupine leghemoglobin gene LllbII, while the desaturase gene Ll3A20 is turned on at least 3 days earlier. Ll3A20 is also expressed in lupine fruits. RT-PCR analysis of the Lldd3A20 and Lldd2T15 expression confirmed organ-specific and temporal pattern of expression in nodules. |
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Thesagro: |
Lupinus Luteus; Tremoço. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01493naa a2200169 a 4500
001 1621193
005 2001-01-18
008 2000 bl --- 0-- u #d
100 1 $aSWIDERSKI, M. R.
245 $aIdentification of new nodulin cDNAs from yellow lupine by differential display.
260 $c2000
520 $aIn this study several sequences that represent yellow lupine nodulin genes are identified. Four different cDNA clones were isolated from a lupine nodule cDNA library and sequenced. Database search revealed that the deduced amino acid of one clone, DD3A20, shows near 80% amino acid similarity to plant stearoyl-acyl carrier protein desaturases and contains crucial amino acids in the fatty acid binding domain which are characteristic for stearoyl-ACP desaturases. The other sequences seem to be unique. Nearly identical signal peptide sequences are present at the N-termini of the DD4A9 and DD2T15 putative poteins. Northern analysis indicated that the pattern of expression of the Lldd4A9, Lldd2A18 and Lldd2T15 genes is the same as that of the lupine leghemoglobin gene LllbII, while the desaturase gene Ll3A20 is turned on at least 3 days earlier. Ll3A20 is also expressed in lupine fruits. RT-PCR analysis of the Lldd3A20 and Lldd2T15 expression confirmed organ-specific and temporal pattern of expression in nodules.
650 $aLupinus Luteus
650 $aTremoço
700 1 $aZABOROWSKA, Z.
700 1 $aLEGOCKI, A. B.
773 $tPlant Science, Ireland$gv. 151, p. 75-83, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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