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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
19/09/2018 |
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Data da última atualização: |
19/09/2018 |
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Autoria: |
MICHEL, U.; KROZOWSKI, Z.; McMASTER, J.; YU, J. H.; FINDLAY, J. K. |
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Título: |
The biphasic modulation of inhibin mRNA levels and secretion by PMSG in rat granulosa cells in vitro. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 3, n. 2, p. 215-226, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Granulosa cell cultures derived from diethylstilboestrol-treated immature rats were used to study the in vitro effect of pregnant mare serum gonadotrophin (PMSG) on steady state mRNA levels for the inhibin alpha and beta A subunits and the secretion of immunoreactive inhibin and progesterone. After 48 h treatment the dose-response curve of PMSG revealed a maximum stimulation (2.5-3.5 fold) of cytosolic alpha and beta A mRNAs over the range of 1 to 10 mU PMSG mL-1, with corresponding stimulation of inhibin secretion. A high dose of PMSG (160-500 mU mL-1) clearly suppressed inhibin alpha mRNA levels as well as inhibin secretion, whereas progesterone (P) was maximally stimulated (up to 600 fold). Although the level of cytosolic inhibin beta A subunit mRNA was also down-regulated by a high concentration of PMSG in the culture medium, the doses required to suppress its mRNA level to less than those of the control varied. These data demonstrate that low doses of follicle stimulating hormone/luteinizing hormone (FSH/LH)-like (PMSG) activity enhances and high doses decrease the steady-state mRNA levels of inhibin in rat granulosa cells in vitro; this biphasic regulation in vitro reflects the differential regulation of inhibin secretion observed during the rat oestrous cycle. |
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Palavras-Chave: |
Drug effects; Gonadotropins Equine; RNA Messenger. |
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Thesaurus Nal: |
Cultured cells; Gene expression regulation; Gonadotropins; Granulosa cells; Kinetics; Pharmacology; Progesterone; Rats; Secretion. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02167naa a2200313 a 4500
001 2096001
005 2018-09-19
008 1991 bl uuuu u00u1 u #d
100 1 $aMICHEL, U.
245 $aThe biphasic modulation of inhibin mRNA levels and secretion by PMSG in rat granulosa cells in vitro.
260 $c1991
520 $aAbstract: Granulosa cell cultures derived from diethylstilboestrol-treated immature rats were used to study the in vitro effect of pregnant mare serum gonadotrophin (PMSG) on steady state mRNA levels for the inhibin alpha and beta A subunits and the secretion of immunoreactive inhibin and progesterone. After 48 h treatment the dose-response curve of PMSG revealed a maximum stimulation (2.5-3.5 fold) of cytosolic alpha and beta A mRNAs over the range of 1 to 10 mU PMSG mL-1, with corresponding stimulation of inhibin secretion. A high dose of PMSG (160-500 mU mL-1) clearly suppressed inhibin alpha mRNA levels as well as inhibin secretion, whereas progesterone (P) was maximally stimulated (up to 600 fold). Although the level of cytosolic inhibin beta A subunit mRNA was also down-regulated by a high concentration of PMSG in the culture medium, the doses required to suppress its mRNA level to less than those of the control varied. These data demonstrate that low doses of follicle stimulating hormone/luteinizing hormone (FSH/LH)-like (PMSG) activity enhances and high doses decrease the steady-state mRNA levels of inhibin in rat granulosa cells in vitro; this biphasic regulation in vitro reflects the differential regulation of inhibin secretion observed during the rat oestrous cycle.
650 $aCultured cells
650 $aGene expression regulation
650 $aGonadotropins
650 $aGranulosa cells
650 $aKinetics
650 $aPharmacology
650 $aProgesterone
650 $aRats
650 $aSecretion
653 $aDrug effects
653 $aGonadotropins Equine
653 $aRNA Messenger
700 1 $aKROZOWSKI, Z.
700 1 $aMcMASTER, J.
700 1 $aYU, J. H.
700 1 $aFINDLAY, J. K.
773 $tReproduction, Fertility and Development$gv. 3, n. 2, p. 215-226, 1991.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| 6. | | MONTANARELLA, L.; MAY, W. B.; TONG, Y.; FONTANA, A.; KLIMANOV, A.; KONTOBOYTSEVA, A.; LOSS, A.; NOOV, B.; LABAZ, B.; SMRECZAK, B.; HONGGUANG, C.; CLERICI, C.; SANCHEZ, C. O.; PENG, D.; TIMOFEEVA, E.; GIASSON, E.; BAZARRADNAA, E.; WEI, F.; FONTES, F.; PEREIRA, G.; ERDOGAN, H. E.; HUSEIN, H. H.; SKRYLNYK, I.; SOBOCKÁ, J.; QU, J.; CLARKE, J. L.; WANG, J.; STUCHI, J. F.; KONYUSHKOVA, M.; SILVA, L. S. da; YAO, L.; VOROTYNTSEVA, L.; SOVETBEK, M.; BOLAÑOS-BENAVIDES, M. M.; ENTZ, M.; ZAKHAROVA, M.; ST. LUCE, M.; SCHELLENBERG, M. P.; SCHELLENBERG, M.; MIROSHNICHENKO, M.; CLARKE, N.; NONGHARNPITAK, N.; NYAMSAMBUU, N.; TURSUNOVNA, O. R.; CARFAGNO, P.; SCHENATO, R. B.; KAPUR, S.; WAN, S.; DYBDAL, S.; BALIUK, S.; KUYPER, T. W.; SHISHKOV, T. A.; BARON, V.; HETMANENKO, V.; CARDONA, W. A.; XU, X.; LIU, X.; GENG, X.; MA, X.; CHEN, X.; ZHANG, Y. Sustainable management of black soils: from practices to policies. In: FAO. Global status of black soils. Rome, 2022. cap. 4, p. 107-144.| Tipo: Capítulo em Livro Técnico-Científico |
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| Registros recuperados : 6 | |
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