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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
15/12/2022 |
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Data da última atualização: |
01/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARRETO, P.; DAMBIRE, C.; SHARMA, G.; VICENTE, J.; OSBORNE, R.; YASSITEPE, J. E. de C. T.; GIBBS, D. J.; MAIA, I. G.; HOLDSWORTH, M. J.; ARRUDA, P. |
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Afiliação: |
PEDRO BARRETO, UNIVERSIDADE ESTADUAL PAULISTA; CHARLENE DAMBIRE, UNIVERSITY OF NOTTINGHAM; GUNJAN SHARMA, UNIVERSITY OF NOTTINGHAM; JORGE VICENTE, UNIVERSITY OF NOTTINGHAM; RORY OSBORNE, UNIVERSITY OF BIRMINGHAM; JULIANA ERIKA DE C T YASSITEPE, CNPTIA; DANIEL J. GIBBS, UNIVERSITY OF BIRMINGHAM; IVAN G. MAIA, UNIVERSIDADE ESTADUAL PAULISTA; MICHAEL J. HOLDSWORTH, UNIVERSITY OF NOTTINGHAM; PAULO ARRUDA, UNIVERSIDADE ESTADUAL DE CAMPINAS. |
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Título: |
Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Current Biology, v. 32, n. 6, p. 1403-1411, Mar. 2022. |
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DOI: |
https://doi.org/10.1016/j.cub.2022.01.037 |
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Idioma: |
Inglês |
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Notas: |
Short communication. Na publicação: Juliana Yassitepe. |
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Conteúdo: |
SUMMARY. Mitochondrial retrograde signaling is an important component of intracellular stress signaling in eukaryotes. UNCOUPLING PROTEIN (UCP)1 is an abundant plant inner-mitochondrial membrane protein with multiple functions including uncoupled respiration and amino-acid transport1,2 that influences broad abiotic stress responses. Although the mechanism(s) through which this retrograde function acts is unknown, overexpression of UCP1 activates expression of hypoxia (low oxygen)-associated nuclear genes.3,4 Here we show in Arabidopsis thaliana that UCP1 influences nuclear gene expression and physiological response by inhibiting the cytoplasmic PLANT CYSTEINE OXIDASE (PCO) branch of the PROTEOLYSIS (PRT)6 N-degron pathway, a major mechanism of oxygen and nitric oxide (NO) sensing.5 Overexpression of UCP1 (UCP1ox) resulted in the stabilization of an artificial PCO N-degron pathway substrate, and stability of this reporter protein was influenced by pharmacological interventions that control UCP1 activity. Hypoxia and salt-tolerant phenotypes observed in UCP1ox lines resembled those observed for the PRT6 N-recognin E3 ligase mutant prt6-1. Genetic analysis showed that UCP1 regulation of hypoxia responses required the activity of PCO N-degron pathway ETHYLENE RESPONSE FACTOR (ERF)VII substrates. Transcript expression analysis indicated that UCP1 regulation of hypoxia-related gene expression is a normal component of seedling development. Our results show that mitochondrial retrograde signaling represses the PCO N-degron pathway, enhancing substrate function, thus facilitating downstream stress responses. This work reveals a novel mechanism through which mitochondrial retrograde signaling influences nuclear response to hypoxia by inhibition of an ancient cytoplasmic pathway of eukaryotic oxygen sensing. MenosSUMMARY. Mitochondrial retrograde signaling is an important component of intracellular stress signaling in eukaryotes. UNCOUPLING PROTEIN (UCP)1 is an abundant plant inner-mitochondrial membrane protein with multiple functions including uncoupled respiration and amino-acid transport1,2 that influences broad abiotic stress responses. Although the mechanism(s) through which this retrograde function acts is unknown, overexpression of UCP1 activates expression of hypoxia (low oxygen)-associated nuclear genes.3,4 Here we show in Arabidopsis thaliana that UCP1 influences nuclear gene expression and physiological response by inhibiting the cytoplasmic PLANT CYSTEINE OXIDASE (PCO) branch of the PROTEOLYSIS (PRT)6 N-degron pathway, a major mechanism of oxygen and nitric oxide (NO) sensing.5 Overexpression of UCP1 (UCP1ox) resulted in the stabilization of an artificial PCO N-degron pathway substrate, and stability of this reporter protein was influenced by pharmacological interventions that control UCP1 activity. Hypoxia and salt-tolerant phenotypes observed in UCP1ox lines resembled those observed for the PRT6 N-recognin E3 ligase mutant prt6-1. Genetic analysis showed that UCP1 regulation of hypoxia responses required the activity of PCO N-degron pathway ETHYLENE RESPONSE FACTOR (ERF)VII substrates. Transcript expression analysis indicated that UCP1 regulation of hypoxia-related gene expression is a normal component of seedling development. Our results show that mitochondrial retrog... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica; Resposta fisiológica. |
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Thesagro: |
Mitocôndria. |
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Thesaurus Nal: |
Arabidopsis thaliana; Gene expression; Gene overexpression; Hypoxia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1149731/1/AP-Mitochondrial-retrograde-2022.pdf
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Marc: |
LEADER 02869naa a2200337 a 4500
001 2149731
005 2023-03-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cub.2022.01.037$2DOI
100 1 $aBARRETO, P.
245 $aMitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway.$h[electronic resource]
260 $c2022
500 $aShort communication. Na publicação: Juliana Yassitepe.
520 $aSUMMARY. Mitochondrial retrograde signaling is an important component of intracellular stress signaling in eukaryotes. UNCOUPLING PROTEIN (UCP)1 is an abundant plant inner-mitochondrial membrane protein with multiple functions including uncoupled respiration and amino-acid transport1,2 that influences broad abiotic stress responses. Although the mechanism(s) through which this retrograde function acts is unknown, overexpression of UCP1 activates expression of hypoxia (low oxygen)-associated nuclear genes.3,4 Here we show in Arabidopsis thaliana that UCP1 influences nuclear gene expression and physiological response by inhibiting the cytoplasmic PLANT CYSTEINE OXIDASE (PCO) branch of the PROTEOLYSIS (PRT)6 N-degron pathway, a major mechanism of oxygen and nitric oxide (NO) sensing.5 Overexpression of UCP1 (UCP1ox) resulted in the stabilization of an artificial PCO N-degron pathway substrate, and stability of this reporter protein was influenced by pharmacological interventions that control UCP1 activity. Hypoxia and salt-tolerant phenotypes observed in UCP1ox lines resembled those observed for the PRT6 N-recognin E3 ligase mutant prt6-1. Genetic analysis showed that UCP1 regulation of hypoxia responses required the activity of PCO N-degron pathway ETHYLENE RESPONSE FACTOR (ERF)VII substrates. Transcript expression analysis indicated that UCP1 regulation of hypoxia-related gene expression is a normal component of seedling development. Our results show that mitochondrial retrograde signaling represses the PCO N-degron pathway, enhancing substrate function, thus facilitating downstream stress responses. This work reveals a novel mechanism through which mitochondrial retrograde signaling influences nuclear response to hypoxia by inhibition of an ancient cytoplasmic pathway of eukaryotic oxygen sensing.
650 $aArabidopsis thaliana
650 $aGene expression
650 $aGene overexpression
650 $aHypoxia
650 $aMitocôndria
653 $aExpressão gênica
653 $aResposta fisiológica
700 1 $aDAMBIRE, C.
700 1 $aSHARMA, G.
700 1 $aVICENTE, J.
700 1 $aOSBORNE, R.
700 1 $aYASSITEPE, J. E. de C. T.
700 1 $aGIBBS, D. J.
700 1 $aMAIA, I. G.
700 1 $aHOLDSWORTH, M. J.
700 1 $aARRUDA, P.
773 $tCurrent Biology$gv. 32, n. 6, p. 1403-1411, Mar. 2022.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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| Registros recuperados : 32 | |
| 4. |  | CAMARGO, I. de; YASSITEPE, J. E. de C. T.; FERNANDES, F. R.; RODRIGUES, G. C. Caracterização fenotípica de linhagens de milho para tolerância à estresse hídrico. In: MOSTRA DE ESTAGIÁRIOS E BOLSISTAS DA EMBRAPA INFORMÁTICA AGROPECUÁRIA, 14., 2018, Campinas. Resumos expandidos... Brasília, DF: Embrapa, 2018. p. 49-53. (Embrapa Informática Agropecuária. Eventos técnicos & científicos, 1). Editores técnicos: Carla Geovana do Nascimento Macário, Carla Cristiane Osawa, Flávia Bussaglia Fiorini, Maria Fernanda Moura, Poliana Fernanda Giachetto.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 5. | | SOUZA, A.; YASSITEPE, J. E. de C. T.; FERNANDES, F. R. Método não destrutivo para isolamento DNA de sementes de milho visando à genotipagem em larga escala. In: MOSTRA DE ESTAGIÁRIOS E BOLSISTAS DA EMBRAPA INFORMÁTICA AGROPECUÁRIA, 14., 2018, Campinas. Resumos expandidos... Brasília, DF: Embrapa, 2018. p. 54-57. (Embrapa Informática Agropecuária. Eventos técnicos & científicos, 1). Editores técnicos: Carla Geovana do Nascimento Macário, Carla Cristiane Osawa, Flávia Bussaglia Fiorini, Maria Fernanda Moura, Poliana Fernanda Giachetto.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 8. | | SANTOS, P. M.; FAVERO, A. P.; PEZZOPANE, J. R. M.; VIGNA, B. B. Z.; YASSITEPE, J. E. de C. T. Coleta, conservação, melhoramento e recomendação de uso de recursos genéticos visando às mudanças do clima. In: SOTTA, E. D.; SAMPAIO, F. G.; MARZALL, K.; SILVA, W. G. da. Estratégias de adaptação às mudanças do clima dos sistemas agropecuários brasileiros. Brasília, DF: MAPA, 2021. p.26-31.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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| 9. | | SANTOS, P. M.; FAVERO, A. P.; PEZZOPANE, J. R. M.; VIGNA, B. B. Z.; YASSITEPE, J. E. de C. T. Collection, conservation, improvement, and recommendations on using genetic resources aiming at climate change. In: SOTTA, E. D.; SAMPAIO, F. G.; MARZALL, K.; SILVA, W . G. da. Adapting to climate change: strategies for brazilian agricultural and livestock system. Brasília, DF: MAPA, 2021. p.26-31.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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| 11. | | BARRETO, P.; KOLTUN, A.; NONATO, J.; YASSITEPE, J. E. de C. T.; MAIA, I. de G.; ARRUDA, P. Metabolism and signaling of plant mitochondria in adaptation to environmental stresses. International Journal of Molecular Sciences, v. 23, n. 19, 11176, 2022. Na publicação: Juliana Yassitepe.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 14. | | PEREIRA, H. D.; RODRIGUES, T. de S.; DANTE, R. A.; GERHARDT, I. R.; YASSITEPE, J. E. de C. T. Searching natural variation for water stress response in maize. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 12., 2023, Caxambu, MG. Anais [...]. Piracicaba: Sociedade Brasileira de Melhoramento de Plantas, 2023. Na publicação: Juliana Erika Yassitepe.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 15. | | LOPES FILHO, J. H.; SILVA, V. C. H. da; SANTOS, J. C. dos; DANTE, R. A.; GERHARDT, I. R.; YASSITEPE, J. E. de C. T.; FERNANDES, F. R. Introdução à edição genômica em plantas: desafios da agricultura moderna para o presente e o futuro. In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. H. (Ed.). Tecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura. Brasília, DF: Embrapa, 2020. cap. 1, p. 11-48.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 16. | | LOPES FILHO, J. H.; SILVA, V. C. H. da; SANTOS, J. C. dos; DANTE, R. A.; GERHARDT, I. R.; YASSITEPE, J. E. de C. T.; FERNANDES, F. R. Introduction to genome editing in plants. In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021. chap. 1, p. 11-46.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 17. | | HERNANDES-LOPES, J.; YASSITEPE, J. E. de C. T.; KOLTUN, A.; PAUWELS, L.; SILVA, V. C. H. da; DANTE, R. A.; GERHARDT, I. R.; ARRUDA, P. Genome editing in maize: toward improving complex traits in a global crop. Genetics and Molecular Biology, v. 46, n. 1, e20220217, 2023. Suplemento 1.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 19. | | BARRETO, P.; DAMBIRE, C.; SHARMA, G.; VICENTE, J.; OSBORNE, R.; YASSITEPE, J. E. de C. T.; GIBBS, D. J.; MAIA, I. G.; HOLDSWORTH, M. J.; ARRUDA, P. Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway. Current Biology, v. 32, n. 6, p. 1403-1411, Mar. 2022. Short communication. Na publicação: Juliana Yassitepe.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 20. | | HELEODORO, T.; RIBEIRO, A. P.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; VINECKY, F.; DE LUCA, P. C.; ARRUDA, P.; DANTE, R. A.; GERHARDT, I. R.; YASSITEPE, J. E. de C. T.; FERNANDES, F. R.; CANÇADO, G. M. de A. Liberação planejada no meio ambiente (LPMA) de plantas transgênicas de cana-de-açúcar. In: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA, 13., Campinas. Anais... [S.l: s.n], 2019 p. 1-9. CIIC 2019. Nº 19605. Na publicação: Hugo Molinari; Adilson A. Kobayashi; Isabel R. Gerhadt; Juliana Erika Teixeira Yassitepe; Fernada R. Fernades; Geraldo M. A. Cançado.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia. |
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| Registros recuperados : 32 | |
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