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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
20/09/2011 |
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Data da última atualização: |
01/03/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; COOKE, P.; WOOD, D. |
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Afiliação: |
CELLI RODRIGUES MUNIZ, CNPAT; FRANCISCO DAS CHAGAS OLIVEIRA FREIRE, CNPAT; FRANCISCO MARTO PINTO VIANA, CNPAT; JOSE EMILSON CARDOSO, CNPAT; PETER COOKE, USDA; DELILAH WOOD, USDA. |
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Título: |
Colonization of cashew plants by Lasiodiplodia theobromae: microscopical features. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Micron, v. 42, n. 5, p. 419-428 , 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Lasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in Brazil. In an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. Lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. Optical microscopy analysis revealed a longitudinally sectioned hyphae located within the xylem vessels, showing an extensive hyphal development in the secondary xylem tissue. SEM images demonstrated that the fungus was found in some asymptomatic samples, particularly within the xylem vessels as confirmed by the optical images. Symptomatic sample images showed an extensive distribution of the fungus along the secondary xylem, within the vessels, infecting xylem parenchyma. A closer look in the secondary xylem parenchyma reveals a heavy and profuse invasion of the cells with a distinguishable cell wall disintegration and fully hyphae dispersal. There was no reactivity of Lasiodiplodia-antisera against mycelial extracts of Colletotrichum gloeosporioides, Phomopsis anardii and Pestalotiopsis guepinii. Following incubation of sections with the polyclonal antisera, the hyphae were intensely and regularly labeled. Rays, vessels and parenchyma cells were the preferred pathway for L. theobromae colonization. Artificial infection provides the information that the vascular cylinder is undoubtedly employed and used by the fungus for hyphae distribution. Immunofluorescence assay employed in situ was applied and the polyclonal antisera produced was able to recognize the fungus and proved to be a sensitive technique to detect it. MenosLasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in Brazil. In an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. Lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. Optical microscopy analysis revealed a longitudinally sectioned hyphae located within the xylem vessels, showing an extensive hyphal development in the secondary xylem tissue. SEM images demonstrated that the fungus was found in some asymptomatic samples, particularly within the xylem vessels as confirmed by the optical images. Symptomatic sample images showed an extensive distribution of the fungus along the secondary xylem, within the vessels, infecting xylem parenchyma. A closer look in the secondary xylem parenchyma reveals a heavy and profuse invasion of the cells with a distinguishable cell wall disintegration and fully hyphae dispersal. There was no reactivity of Lasiodiplodia-antisera against mycelial extracts of Colletotrichum gloeosporioides, Phomopsis anardii and Pestalotiopsis guepinii. Following incubation of sections with the polyclonal antisera, the hyphae were intensely and regularly labeled. Rays, vessels and parenchyma cells were the preferred pathway for L. theobromae colonization. Artificial in... Mostrar Tudo |
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Palavras-Chave: |
Anacardium occidentale L; Cell wall degradation; Endophytic fungi; Immunofluorescence. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02449naa a2200229 a 4500
001 1900945
005 2013-03-01
008 2011 bl uuuu u00u1 u #d
100 1 $aMUNIZ, C. R.
245 $aColonization of cashew plants by Lasiodiplodia theobromae$bmicroscopical features.
260 $c2011
520 $aLasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in Brazil. In an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. Lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. Optical microscopy analysis revealed a longitudinally sectioned hyphae located within the xylem vessels, showing an extensive hyphal development in the secondary xylem tissue. SEM images demonstrated that the fungus was found in some asymptomatic samples, particularly within the xylem vessels as confirmed by the optical images. Symptomatic sample images showed an extensive distribution of the fungus along the secondary xylem, within the vessels, infecting xylem parenchyma. A closer look in the secondary xylem parenchyma reveals a heavy and profuse invasion of the cells with a distinguishable cell wall disintegration and fully hyphae dispersal. There was no reactivity of Lasiodiplodia-antisera against mycelial extracts of Colletotrichum gloeosporioides, Phomopsis anardii and Pestalotiopsis guepinii. Following incubation of sections with the polyclonal antisera, the hyphae were intensely and regularly labeled. Rays, vessels and parenchyma cells were the preferred pathway for L. theobromae colonization. Artificial infection provides the information that the vascular cylinder is undoubtedly employed and used by the fungus for hyphae distribution. Immunofluorescence assay employed in situ was applied and the polyclonal antisera produced was able to recognize the fungus and proved to be a sensitive technique to detect it.
653 $aAnacardium occidentale L
653 $aCell wall degradation
653 $aEndophytic fungi
653 $aImmunofluorescence
700 1 $aFREIRE, F. das C. O.
700 1 $aVIANA, F. M. P.
700 1 $aCARDOSO, J. E.
700 1 $aCOOKE, P.
700 1 $aWOOD, D.
773 $tMicron$gv. 42, n. 5, p. 419-428 , 2011.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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| Registros recuperados : 40 | |
| 1. |  | MALMONGE, J. A.; MEDEIRO, E. S.; ROSA, M. F.; WOOD, D. F.; MATTOSO, L. H. C. Compósito de borracha natural proveniente da guayule reforçado com microfibras de celulose. In: CONGRESSO BRASILEIRO DE POLIMEROS, 10., 2009, Foz do Iguaçu, PR. Livro de resumos... São Carlos: ABPol, 2009. p. 106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 2. | | MALMONGE, J. A.; MEDEIROS, E. S.; ROSA, M. F.; WOOD, D.; MATTOSO, L. H. C. Compósito de borracha natural proveniente da guayule reforçado com microfibras de celulose. In: CONGRESSO BRASILEIRO DE POLIMEROS, 10., 2009, Foz do Iguaçu, PR. Anais... São Carlos: ABPol , 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 6. | | MATTOSO, L. H. C.; BAKER, D. A.; AVLONI, J.; WOOD, D. F.; ORTS, W. J. Nanocompistes made from cellulose and polyaniline. In: MACRO 2006; WORLD POLYMER CONGRESS, Rio de Janeiro, Brazil; INTERNATIONAL SYMPOSIUM ON MACROMOLECULES, 41ST., 2006, Rio de Janeiro. Program: abstracts... [S. l.]: ABPol, 2006. ref. 1637.| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | ROSA, M. F.; CHIOU, B.; MEDEIROS, E. S.; WOOD, D. F.; MATTOSO, L. H. C.; ORTS, W. J.; IMAM, S. Biodegradable composites based on starch/EVA blends and coir. In: CLEAN TECHNOLOGY AND SUSTENTAINABLE INDUSTRIES CONFERENCE, 11., 2008, Boston. Abstract... Boston: Nano Science and Technology Institute, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 8. | | ROSA, M. F.; CHIOU, B.; MEDEIROS, E. S.; WOOD, D. F.; MATTOSO, L. H. C.; ORTS, W. J.; IMAM, S. Biodegradable composites based on starch/EVOH/glycerol blends and coconut fibers. Journal of Applied Polymer Science, v. 111, n. 2, p. 612-618, 2008. DOI: 10.1002/app.29| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 9. | | MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; COOKE, P.; WOOD, D. Colonization of cashew plants by Lasiodiplodia theobromae: microscopical features. Micron, v. 42, n. 5, p. 419-428 , 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|  |
| 10. | | ROSA, M. F.; CHIOU, B.; MEDEIROS, E. S.; WOOD, D. F.; MATTOSO, L. H. C.; ORTS, W. J.; IMAM, S. Composite of immature cor fibers and starch/EVOH/glycerol blends. In: BRAZILIAN MRS MEETING, 7., 2008, Guarujá. Resumos... Guarujá: Sociedade Brasileira de Pesquisa de Materiais, 2008. VII Encontro SBPMat.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 11. | | MALMONGE, J. A.; MEDEIROS, E. S.; ROSA, M. de F.; WOOD, D. F.; MATTOSO, L. H. C.; McMAHAN, C. M. Compósito de borracha natural proveniente da guayule reforçado com microfibras de celulose. In: CONGRESSO BRASILEIRO DE POLÍMEROS, 10., 2009, Foz do Iguaçu. Anais... São Carlos: Associação Brasileira de Polímeros, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|  |
| 12. | | MATTOSO, L. H. C.; MEDEIROS, E. S.; BAKER, D. A.; AVLONI, J.; WOOD, D. F.; ORTS, W. J. Electrically conductive nanocomposites made from cellulose nanofibrils and polyaniline. Journal of nanoscience and nanotechnology, Stevenson Ranch, v. 9, n. 5, p. 2917-2922, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | AZEREDO, H. M. C.; MATTOSO, L. H. C.; WOOD, D.; WILLIAMS, T. G.; AVENA-BUSTILLOS, R. J.; MCHUGH, T. H. Nanocomposite edible films from mango puree reinforced with cellulose nanofibers. Journal of food science, Chicago, v. 74, n. 5, p. N31-N35, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | MIRANDA, M.; SUN, X.; FERENCE, C.; PLOTTO, A.; BAI, J.; WOOD, D.; ASSIS, O. B. G. de; FERREIRA, M. D. Nano- and Micro- Carnauba Wax Emulsions versus Shellac Protective Coatings on Postharvest Citrus Quality. Journal of the American Society for Horticultural Science,v. 146, n. 1, 2021. 40-49| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 15. | | MARTINS, M. A.; McMAHAN, C. M.; WOOD, D. F.; MORENO, R. M. B.; MATTOSO, L. H. C.; GONÇALVES, P. S. Particle size investigation on natural rubber from different clones of brazilian IAC series. In: INTERNATIONAL MACROMOLECULAR COLLOQUIUM - IMC, 11.; INTERNATIONAL SYMPOSIUM ON NATURAL POLYMERS AND COMPOSITES - ISNaPol, 6., 2007, Gramado, RS. Natural polymers and composites. Anais... [São Carlos: ABPol, 2007]. não paginado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | MARTINS, M. A.; McMAHAN, C. M.; WOOD, D. F.; MORENO, R. M. B.; MATTOSO, L. H. C.; GONÇALVES, P. S. Particle size investigation on natural rubber from different clones of brazilian IAC series. In: INTERNATIONAL MACROMOLECULAR COLLOQUIUM - IMC, 11.; INTERNATIONAL SYMPOSIUM ON NATURAL POLYMERS AND COMPOSITES - ISNaPol, 6., 2007, Gramado, RS. Natural polymers and composites. Program and proceedings... [São Carlos: ABPol, 2007]. p. Poster 83.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 18. | | CAMPOS, A. de; MARCONCINI, J. M.; IMAM, S. H.; KLAMCZYNSKI, A.; ORTIS, W. J.; WOOD, D. H.; WILLIAMS, T. G.; MARTINS-FRANCHETTI, S. M.; MATTOSO, L. H. C. Biodegradação e propriedades mecânicas e morfológicas do biocompósito de amido termoplástico e policaprolactona com fibras de sisal. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 294-296 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 19. | | ROSA, M. F.; MEDEIROS, E. S.; MALMONGE, J. A.; WOOD, D. F.; MATTOSO, L. H. C.; ORTS, W. J.; IMAM, S. H. Cellulose nanocrystals from coconut fiber: preparation and characterization. In: INTERNATIONAL CONFERENCE ON ADVANCED MATERIALS - ICAM, 11.; ENCONTRO SBPMat, 8., 2009, Rio de Janeiro. Abstracts... Rio de Janeiro: SBPMat, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Instrumentação. |
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| 20. | | ROSA, M. de F.; MEDEIROS, E. S.; MALMONGE, J. A.; GREGORSKI, K. S.; WOOD, D. F.; MATTOSO, L. H. C.; GLENN, G.; ORTS, W. J.; IMAM, S. H. Cellulose nanowhiskers from coconut husk fibers: Effect of preparation conditions on their thermal and morphological behavior. Carbohydrate Polymers, Leiden (Holanda), v. 81, n. 1, p. 83-92, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| Registros recuperados : 40 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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