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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
21/12/2011 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAMARGO, L. S. de A.; BOITE, M. C.; WOHLRES-VIANA, S.; MOTA, G. B.; SERAPIÃO, R. V.; SÁ, W. F. de; VIANA, J. H. M.; NOGUEIRA, L. A. G. |
Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARIANA CORTES BOITE, FIOCRUZ; SABINE WOHLRES-VIANA, UFJF; GUSTAVO BRUNO MOTA, UNB; RAQUEL VARELA SERAPIÃO, PESAGRO; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL; LUIZ ALTAMIRO GARCIA NOGUEIRA, UFF. |
Título: |
Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Cryobiology, v. 63, n. 3, p. 256-262, 2011. |
DOI: |
https://doi.org/10.1016/j.cryobiol.2011.09.135 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. MenosThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-... Mostrar Tudo |
Palavras-Chave: |
Embryo. |
Thesaurus Nal: |
cryopreservation; osmosis; vitrification. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02924naa a2200265 a 4500 001 1910654 005 2024-02-05 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.cryobiol.2011.09.135$2DOI 100 1 $aCAMARGO, L. S. de A. 245 $aOsmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro.$h[electronic resource] 260 $c2011 520 $aThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. 650 $acryopreservation 650 $aosmosis 650 $avitrification 653 $aEmbryo 700 1 $aBOITE, M. C. 700 1 $aWOHLRES-VIANA, S. 700 1 $aMOTA, G. B. 700 1 $aSERAPIÃO, R. V. 700 1 $aSÁ, W. F. de 700 1 $aVIANA, J. H. M. 700 1 $aNOGUEIRA, L. A. G. 773 $tCryobiology$gv. 63, n. 3, p. 256-262, 2011.
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Registros recuperados : 44 | |
21. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N; MACHADO, M. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; VIANA, J. H. M. Intrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus. Reproduction Fertility and Development, v. 29, n. 10, p. 1958-1970, 2017. Publicado online em 19 nov. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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23. | | COSTA, F. Q.; PEREIRA, M. M.; WOHLRES VIANA, S.; SERAPIAO, R. V.; CARVALHO, B. C.; VIANA, J. H. M.; NOGUEIRA, L. A. G.; CAMARGO, L. S. de A. Effect of heat stress during oocyte maturation on gene expression of in vitro-fertilized and parthenogenetic bovine blastocysts. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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24. | | WOHLRES-VIANA, S.; BOITE, M. C.; PEREIRA, M. M.; SA, W. F. de; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. Effect of production system and breed on relative expression in bovine embryos In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceeding... San Diego: Csiro, 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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25. | | ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. Reproductive Biology and Endocrinology, v. 11, article 73, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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26. | | RODRIGUES, M. F.; ALVES, C. C. S.; FIGUEIREDO, B. B. M.; REZENDE, A. B.; WOHLRES-VIANA, S.; SILVA, V. L.; MACHADO, M. A.; TEIXEIRA, H. C. Tumour necrosis factor receptors and apoptosis of alveolar macrophages during early infection with attenuaed and virulent Mycobacterium bovis. Immunology, v. 139, n. 4, p. 503-512, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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28. | | CAMARGO, L. S. de A.; PEREIRA, M. M.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; IGUMA, L. T.; CARVALHO, B. C. de; SIQUEIRA, L. G. B.; VIANA, J. H. M. Altered gene expression in bovine somatic cell nuclear-transfered embryos after trichostatin a treatment. Reproduction, Fertility and Development, v. 24, n. 1, p. 121-122, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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29. | | MINARÉ, T. P.; ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CORDEIRO, C. E. C.; FERNANDES, C. A. C.; VIANA, J. H. M. Characterization of individual differences in the efficiency of superovulation in the Gyr breed. Animal Reproduction, v.10, n.3, p. 546, Jul./Sept. 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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30. | | DOMINGUES, R.; WOHLRES-VIANA, S.; REIS, D. R. de L.; TEIXEIRA, H. C.; FERREIRA, A. P.; GUIMARÃES, S. E. F.; PRATA, M. C. de A.; FURLONG, J.; VERNEQUE, R. da S.; MACHADO, M. A. Expression of immune response genes in peripheral blood of cattle infested with Rhipicephalus microplus. Genetics and Molecular Research, v. 13, n. 2, p. 4013-21, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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31. | | ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; PALHÃO, M. P.; RABELO, N. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PAIM, C. A. S.; VIANA, J. H. M. Follicular fluid and granulosa cell recovery from bovine follicles of different diameters using an adapted transvaginal-guided follicular aspiration system. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 38., 2012, Phoenix, Arizona. Abstracts... [S.l.: s.n.], 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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32. | | CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M. Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Reproduction in Domestic Animals, v. 54, n. 10, p. 1357-1365, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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33. | | BOITÉ, M. C.; NOGUEIRA, L. A. G.; PEREIRA, M. M.; BATISTA, R. I. T. P.; WOHLRES-VIANA, S.; POLISSENI, J.; SERAPIÃO, R. V.; SÁ, W. F.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. A. Comportamento osmótico de embriões bovinos cultivados em meio SOFaa ou CR2aa. Acta Scientiae Veterinariae, v. 35, p. s997, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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34. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. Theriogenology, v. 126, p. 68-74, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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35. | | PEREIRA, M. M.; WOHLRES-VIANA, S.; SALES, J. N. S.; CAMARGO, A. R.; QUINTAO, C. C. R.; RABELO, N. C.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A.; GUIMARAES, M. F. M.; IGUMA, L. T. Differential gene expression od in vitro-matured bovine oocytes qith od without a polar body. In: ANNUAL CONFERENCE OF THE INTERNACIONAL EMBRYO TRANSFES SOCIETY, 2012, Phoenix. Proceedings... [S.l.:s.n.], 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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36. | | BATISTA, R. I. T. P.; FAZA, A. P.; WOHLRES-VIANA, S.; PINTO, I. S. B.; MORAES, M. N. de C. M.; LIMA, K. D. A.; CAMARGO, L. S. A.; VIANA, J. H. M. Resposta superovulatória em camundongos F1 produzidos a partir de diferentes estratégias de cruzamento entre linhagens endogâmicas. Acta Scientiae Veterinariae, v. 35, p. s1299, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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37. | | CAMPOS JÚNIOR, P. H. A.; CARVALHO, B. C.; SERAPIÃO, R. V.; PEREIRA, M. M.; COSTA, F. Q.; POLISSENI, J.; WOHLRES-VIANA, S.; SÁ, W. F.; VIANA, J. H. M.; CAMARGO, L. S. Produção e criotolerância de embriões bovinos produzidos sob diferentes sistemas de cultivo. In: 31ª SEMANA DE BIOLOGIA. MOSTRA DE PRODUÇÃO CIENTÍFICA. 14. 2008, Anais. Juiz de Fora. UFJF, 2008. 4 p. 1 CD.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Gado de Leite. |
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38. | | PEREIRA, M. M.; BATISTA, R. I. T. P.; WOHLRES-VIANA, S.; BOITÉ, M. C.; SERAPIÃO, R. V.; POLISSENI, J.; IGUMA, L. T.; SÁ, W. F.; CAMARGO, L. S. A.; VIANA, J. H. M. Relação entre qualidade morfológica de complexos cumulus-oócitos bovinos e potencial de desenvolvimento in vitro. Acta Scientiae Veterinariae, v. 35, p. s1182, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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39. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; SUDRÉ, A. P.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; MACHADO, M. A.; VIANA, J. H. M. Luteinizing hormone receptor gene expression during follicular divergence in B. taurus vs B. indicus dairy breeds. Animal Reproduction, v.10, n.3, p. 425, Jul./Sept. 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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40. | | CAMARGO, L. S. de A.; BOITE, M. C.; WOHLRES-VIANA, S.; MOTA, G. B.; SERAPIÃO, R. V.; SÁ, W. F. de; VIANA, J. H. M.; NOGUEIRA, L. A. G. Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. Cryobiology, v. 63, n. 3, p. 256-262, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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