| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
14/10/2010 |
|
Data da última atualização: |
30/06/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PEDROSO, A. de F.; ADESOGAN, A. T.; QUEIROZ, O. C. M.; WILLIAMS, S. K. |
|
Afiliação: |
ANDRE DE FARIA PEDROSO, CPPSE; A. T. ADESOGAN, UNIVERSITY OF FLORIDA/GAINESVILLE; O. C. M. QUEIROZ, UNIVERSITY OF FLORIDA/GAINSVILLE; S. K. WILLIAMS, UNIVERSITY OF FLORIDA/GAINSVILLE. |
|
Título: |
Control of Escherichia coli O157:H7 in corn silage with or without various inoculants: Efficacy and mode of action. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Journal of Dairy Science, v. 93, n. 3, p. 1098-1104, 2010. |
|
Páginas: |
p. 402 |
|
DOI: |
10.3168/jds.2009-2433 |
|
Idioma: |
Português |
|
Conteúdo: |
This study aimed to evaluate the effectiveness of 3 commercial bacterial inoculants at controlling Escherichia coli O157:H7 in corn silages during ensiling and feedout phases of silage production. A second objective was to determine whether the inoculants exhibited and transferred antibacterial activity against E. coli O157:H7 to the silages. Chopped corn forage was ensiled after treatment with the following: distilled water (control); 5 × 105 cfu/g of E. coli O157:H7 (EC); EC and 1 × 106 cfu/g of Pediococcus pentosaceus and Propionibacterium freudenreichii (EC+BII); EC and 1 × 106 cfu/g of Lactobacillus buchneri (EC+LB); and EC and 1 × 106 cfu/g of L. buchneri and P. pentosaceus (EC+B500). Each treatment was ensiled in triplicate in mini silos for 3, 7, 31, and 82 d and analyzed for pH and E. coli O157:H7 counts. Samples from d 82 were also analyzed for volatile fatty acids, lactate, and aerobic stability. Antibacterial activity of inoculants and silages was determined by the Kirby-Bauer disc diffusion test. The pH of silages from all treatments decreased below 4 within 3 d of ensiling and remained low until d 82. Therefore, E. coli O157:H7 was not detected in silages after any of the ensiling durations. Applying inoculants containing L. buchneri resulted in less lactate, more acetate, and greater aerobic stability compared with the control. Applying EC+BII containing P. freudenreichii did not increase propionate or aerobic stability. Subsamples of d 82 silages were reinoculated with 1 × 105 cfu/g of E. coli O157:H7 either immediately after silo opening on d 82 or after 144h of aerobic exposure (d 88), and E. coli were enumerated 24h later. All silages reinoculated with the pathogen on d 82 had similar, low pH values (<4) and no E. coli were detected 24h later. Control, EC, and EC+BII silages reinoculated with the pathogen after 144h of aerobic exposure had relatively greater pH values (4.71, 5.67, and 6.03, respectively) and E. coli counts (2.87, 6.73, and 6.87 log cfu/g, respectively) 24h later, whereas those treated with L. buchneri had low pH values (<4) and undetectable (EC+B500) or 10-fold lower (1.97, cfu/g; EC+LB) E. coli counts. All pure cultures of commercial bacterial inoculants exhibited antibacterial activity independent of pH against E. coli O157:H7, but the pH-independent activity did not persist in the treated silages, suggesting that E. coli elimination from silages was mediated by pH reduction. MenosThis study aimed to evaluate the effectiveness of 3 commercial bacterial inoculants at controlling Escherichia coli O157:H7 in corn silages during ensiling and feedout phases of silage production. A second objective was to determine whether the inoculants exhibited and transferred antibacterial activity against E. coli O157:H7 to the silages. Chopped corn forage was ensiled after treatment with the following: distilled water (control); 5 × 105 cfu/g of E. coli O157:H7 (EC); EC and 1 × 106 cfu/g of Pediococcus pentosaceus and Propionibacterium freudenreichii (EC+BII); EC and 1 × 106 cfu/g of Lactobacillus buchneri (EC+LB); and EC and 1 × 106 cfu/g of L. buchneri and P. pentosaceus (EC+B500). Each treatment was ensiled in triplicate in mini silos for 3, 7, 31, and 82 d and analyzed for pH and E. coli O157:H7 counts. Samples from d 82 were also analyzed for volatile fatty acids, lactate, and aerobic stability. Antibacterial activity of inoculants and silages was determined by the Kirby-Bauer disc diffusion test. The pH of silages from all treatments decreased below 4 within 3 d of ensiling and remained low until d 82. Therefore, E. coli O157:H7 was not detected in silages after any of the ensiling durations. Applying inoculants containing L. buchneri resulted in less lactate, more acetate, and greater aerobic stability compared with the control. Applying EC+BII containing P. freudenreichii did not increase propionate or aerobic stability. Subsamples of d 82 silages were reinocu... Mostrar Tudo |
|
Palavras-Chave: |
Antibacterial activity; Bacterial inoculant. |
|
Thesaurus Nal: |
Escherichia coli O157; silage. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 03190naa a2200229 a 4500
001 1864172
005 2023-06-30
008 2010 bl uuuu u00u1 u #d
024 7 $a10.3168/jds.2009-2433$2DOI
100 1 $aPEDROSO, A. de F.
245 $aControl of Escherichia coli O157$bH7 in corn silage with or without various inoculants: Efficacy and mode of action.$h[electronic resource]
260 $c2010
300 $ap. 402
520 $aThis study aimed to evaluate the effectiveness of 3 commercial bacterial inoculants at controlling Escherichia coli O157:H7 in corn silages during ensiling and feedout phases of silage production. A second objective was to determine whether the inoculants exhibited and transferred antibacterial activity against E. coli O157:H7 to the silages. Chopped corn forage was ensiled after treatment with the following: distilled water (control); 5 × 105 cfu/g of E. coli O157:H7 (EC); EC and 1 × 106 cfu/g of Pediococcus pentosaceus and Propionibacterium freudenreichii (EC+BII); EC and 1 × 106 cfu/g of Lactobacillus buchneri (EC+LB); and EC and 1 × 106 cfu/g of L. buchneri and P. pentosaceus (EC+B500). Each treatment was ensiled in triplicate in mini silos for 3, 7, 31, and 82 d and analyzed for pH and E. coli O157:H7 counts. Samples from d 82 were also analyzed for volatile fatty acids, lactate, and aerobic stability. Antibacterial activity of inoculants and silages was determined by the Kirby-Bauer disc diffusion test. The pH of silages from all treatments decreased below 4 within 3 d of ensiling and remained low until d 82. Therefore, E. coli O157:H7 was not detected in silages after any of the ensiling durations. Applying inoculants containing L. buchneri resulted in less lactate, more acetate, and greater aerobic stability compared with the control. Applying EC+BII containing P. freudenreichii did not increase propionate or aerobic stability. Subsamples of d 82 silages were reinoculated with 1 × 105 cfu/g of E. coli O157:H7 either immediately after silo opening on d 82 or after 144h of aerobic exposure (d 88), and E. coli were enumerated 24h later. All silages reinoculated with the pathogen on d 82 had similar, low pH values (<4) and no E. coli were detected 24h later. Control, EC, and EC+BII silages reinoculated with the pathogen after 144h of aerobic exposure had relatively greater pH values (4.71, 5.67, and 6.03, respectively) and E. coli counts (2.87, 6.73, and 6.87 log cfu/g, respectively) 24h later, whereas those treated with L. buchneri had low pH values (<4) and undetectable (EC+B500) or 10-fold lower (1.97, cfu/g; EC+LB) E. coli counts. All pure cultures of commercial bacterial inoculants exhibited antibacterial activity independent of pH against E. coli O157:H7, but the pH-independent activity did not persist in the treated silages, suggesting that E. coli elimination from silages was mediated by pH reduction.
650 $aEscherichia coli O157
650 $asilage
653 $aAntibacterial activity
653 $aBacterial inoculant
700 1 $aADESOGAN, A. T.
700 1 $aQUEIROZ, O. C. M.
700 1 $aWILLIAMS, S. K.
773 $tJournal of Dairy Science$gv. 93, n. 3, p. 1098-1104, 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
20/05/1998 |
|
Data da última atualização: |
25/04/2019 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
QUEIROZ, M. A. de; SOUZA, F. de F. |
|
Afiliação: |
MANOEL ABÍLIO DE QUEIROZ, CPATSA; FLAVIO DE FRANCA SOUZA. |
|
Título: |
Selecao e linhagens de melancia para cultivo irrigado no semi-arido brasileiro. |
|
Ano de publicação: |
1998 |
|
Fonte/Imprenta: |
In: ENCONTRO DE GENETICA DO NORDESTE, 13., 1998, Feira de Santana. Anais... Feira de Santana: SBG: UEFS, 1998. |
|
Páginas: |
p. 368. |
|
Idioma: |
Português |
|
Conteúdo: |
Alguns parentais promissores de melancia (Citrullus lanatus (Thunb.) Matsun. & Nakai) foram escolhidos para o desenvolvimento de linahgens produtivas e de boas caracteristicas de fruto. As plantas selecionadas são autofecundadas sucessivamente. Trinta proogenies, provenientes de autofecundacao de diferentes parentais, foram avaliadas quanto aaos seguintes caracteres; peso de frutos; teor de solidos soluveis; formato do fruto; espessura, cor e padrao de casca; progenie foram avaliadas. O paternal PE-13 apresentou frutos com peso acima de 13 kg e teor de solidos soluveis acima de 10 obrix. As demais progenies apresentaram peso medio variando de 1,9 a 7,5 kg e teor medio de solidos soluveis e alongados, quanto ao formato, e lisstrados, rendilhados e uniformes, quanto ao padrao de casca. A cor da casca variou de verde-claro a verde escuro, e a cor da polpa variou de branca a vermelha. A espessura media da casca variou de 0,8 a 1,9 cm e a prolificidade media, de 2,5 a 14,8 frutos/planta. As primeiras flores masculina e feminina apareceram, em media, entre 39 e 51 dias e entre 43 e 56 dias, apos o plantio, respectivamente. As treze melhores progenies, quanto as caracteristicas avaliadas, foram selecionadas para serem novamente autofecundadas, e assim dar continuidade ao programa de melhoramento. |
|
Palavras-Chave: |
Regiao semi-arida; Semi-arid region. |
|
Thesagro: |
Citrullus Lanatus; Irrigação; Linhagem; Melancia. |
|
Thesaurus NAL: |
Brazil; irrigation; progeny; watermelons. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196423/1/Encontro-de-Genetica-do-nordeste-pag-368.pdf
|
|
Marc: |
LEADER 02043nam a2200253 a 4500
001 1132955
005 2019-04-25
008 1998 bl uuuu u00u1 u #d
100 1 $aQUEIROZ, M. A. de
245 $aSelecao e linhagens de melancia para cultivo irrigado no semi-arido brasileiro.
260 $aIn: ENCONTRO DE GENETICA DO NORDESTE, 13., 1998, Feira de Santana. Anais... Feira de Santana: SBG: UEFS$c1998
300 $ap. 368.
520 $aAlguns parentais promissores de melancia (Citrullus lanatus (Thunb.) Matsun. & Nakai) foram escolhidos para o desenvolvimento de linahgens produtivas e de boas caracteristicas de fruto. As plantas selecionadas são autofecundadas sucessivamente. Trinta proogenies, provenientes de autofecundacao de diferentes parentais, foram avaliadas quanto aaos seguintes caracteres; peso de frutos; teor de solidos soluveis; formato do fruto; espessura, cor e padrao de casca; progenie foram avaliadas. O paternal PE-13 apresentou frutos com peso acima de 13 kg e teor de solidos soluveis acima de 10 obrix. As demais progenies apresentaram peso medio variando de 1,9 a 7,5 kg e teor medio de solidos soluveis e alongados, quanto ao formato, e lisstrados, rendilhados e uniformes, quanto ao padrao de casca. A cor da casca variou de verde-claro a verde escuro, e a cor da polpa variou de branca a vermelha. A espessura media da casca variou de 0,8 a 1,9 cm e a prolificidade media, de 2,5 a 14,8 frutos/planta. As primeiras flores masculina e feminina apareceram, em media, entre 39 e 51 dias e entre 43 e 56 dias, apos o plantio, respectivamente. As treze melhores progenies, quanto as caracteristicas avaliadas, foram selecionadas para serem novamente autofecundadas, e assim dar continuidade ao programa de melhoramento.
650 $aBrazil
650 $airrigation
650 $aprogeny
650 $awatermelons
650 $aCitrullus Lanatus
650 $aIrrigação
650 $aLinhagem
650 $aMelancia
653 $aRegiao semi-arida
653 $aSemi-arid region
700 1 $aSOUZA, F. de F.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
