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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
07/11/2014 |
Data da última atualização: |
23/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RODRIGUES, T. B.; KHAJURIA, C.; WANG, H.; MATZ, N.; CARDOSO, D. C.; VALICENTE, F. H.; ZHOU, X.; SIEGFRIED, B. |
Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
Título: |
Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera). |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plos One, San Francisco,v. 9, n. 10, p. 1-8, Oct. 2014. |
DOI: |
10.1371/journal.pone.0109825 |
Idioma: |
Inglês |
Conteúdo: |
Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation of qRT-PCR results difficult. In this study, four distinct algorithms (Genorm, NormFinder, BestKeeper and delta-CT) and five candidate HKGs to genes of reference (b-actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; b-tubulin; RPS9, ribosomal protein S9; EF1a, elongation factor-1a) were evaluated to determine the most reliable HKG under different experimental conditions including exposure to dsRNA and Bt toxins and among different tissues and developmental stages. Although all the HKGs tested exhibited relatively stable expression among the different treatments, some differences were noted. Among the five candidate reference genes evaluated, b-actin exhibited highly stable expression among different life stages. RPS9 exhibited the most similar pattern of expression among dsRNA treatments, and both experiments indicated that EF1a was the second most stable gene. EF1a was also the most stable for Bt exposure and among different tissues. These results will enable researchers to use more accurate and reliable normalization of qRT-PCR data in WCR experiments. MenosQuantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation of qRT-PCR results difficult. In this study, four distinct algorithms (Genorm, NormFinder, BestKeeper and delta-CT) and five candidate HKGs to genes of reference (b-actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; b-tubulin; RPS9, ribosomal protein S9; EF1a, elongation factor-1a) were evaluated to determine the most reliable HKG under different experimental conditions including exposure to dsRNA and Bt toxins and among different tissues and developmental stages. Although all the HKGs tested exhibited relatively stable expression among the different treatments, some differences were noted. Among the five candidate reference genes evaluated, b-actin exhibited highly stable expression a... Mostrar Tudo |
Thesagro: |
Gene; Genética; Lagarta; Praga de planta. |
Thesaurus Nal: |
Plant pests. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/111142/1/Validation-reference.pdf
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Marc: |
LEADER 02688naa a2200277 a 4500 001 1999434 005 2017-05-23 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0109825$2DOI 100 1 $aRODRIGUES, T. B. 245 $aValidation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).$h[electronic resource] 260 $c2014 520 $aQuantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation of qRT-PCR results difficult. In this study, four distinct algorithms (Genorm, NormFinder, BestKeeper and delta-CT) and five candidate HKGs to genes of reference (b-actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; b-tubulin; RPS9, ribosomal protein S9; EF1a, elongation factor-1a) were evaluated to determine the most reliable HKG under different experimental conditions including exposure to dsRNA and Bt toxins and among different tissues and developmental stages. Although all the HKGs tested exhibited relatively stable expression among the different treatments, some differences were noted. Among the five candidate reference genes evaluated, b-actin exhibited highly stable expression among different life stages. RPS9 exhibited the most similar pattern of expression among dsRNA treatments, and both experiments indicated that EF1a was the second most stable gene. EF1a was also the most stable for Bt exposure and among different tissues. These results will enable researchers to use more accurate and reliable normalization of qRT-PCR data in WCR experiments. 650 $aPlant pests 650 $aGene 650 $aGenética 650 $aLagarta 650 $aPraga de planta 700 1 $aKHAJURIA, C. 700 1 $aWANG, H. 700 1 $aMATZ, N. 700 1 $aCARDOSO, D. C. 700 1 $aVALICENTE, F. H. 700 1 $aZHOU, X. 700 1 $aSIEGFRIED, B. 773 $tPlos One, San Francisco,v. 9$gn. 10, p. 1-8, Oct. 2014.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
20/12/2017 |
Data da última atualização: |
27/12/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
GREGOSKI, C.; BRUGNERA, L.; TREVISAN, C.; SANTOS, G. B. dos; CUNHA, G. R. da; DALMAGO, G. A.; GOUVÊA, J. A. de; FERNANDES, J. M. C.; PASINATO, A. |
Afiliação: |
CRISTIAN GREGOSKI, Agronomia - UPF; LUANA BRUGNERA, Agronomia - IDEAU; CLEITO TREVISAN, Agronomia - IDEAU; GUSTAVO BILIBIO DOS SANTOS, Agronomia - UPF; GILBERTO ROCCA DA CUNHA, CNPT; GENEI ANTONIO DALMAGO, CNPT; JORGE ALBERTO DE GOUVÊA, CNPT; JOSÉ MAURÍCIO CUNHA FERNANDES, CNPT; ALDEMIR PASINATO, CNPT. |
Título: |
Produtividade da canola em função da disponibilidade de água no solo. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 12.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 9., 2017, Passo Fundo. Resumos... Passo Fundo: Embrapa Trigo, 2017. |
Páginas: |
p. 25. |
Idioma: |
Português |
Palavras-Chave: |
Brassica napus var napus. |
Thesagro: |
Brassica napus; Colza; Manejo de água; Rendimento. |
Thesaurus NAL: |
Canola; Water management; Yields. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169441/1/2017MICp25.pdf
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Marc: |
LEADER 00944nam a2200301 a 4500 001 2083113 005 2017-12-27 008 2017 bl uuuu u00u1 u #d 100 1 $aGREGOSKI, C. 245 $aProdutividade da canola em função da disponibilidade de água no solo.$h[electronic resource] 260 $aIn: MOSTRA DE INICIAÇÃO CIENTÍFICA, 12.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 9., 2017, Passo Fundo. Resumos... Passo Fundo: Embrapa Trigo$c2017 300 $ap. 25. 650 $aCanola 650 $aWater management 650 $aYields 650 $aBrassica napus 650 $aColza 650 $aManejo de água 650 $aRendimento 653 $aBrassica napus var napus 700 1 $aBRUGNERA, L. 700 1 $aTREVISAN, C. 700 1 $aSANTOS, G. B. dos 700 1 $aCUNHA, G. R. da 700 1 $aDALMAGO, G. A. 700 1 $aGOUVÊA, J. A. de 700 1 $aFERNANDES, J. M. C. 700 1 $aPASINATO, A.
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Embrapa Trigo (CNPT) |
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