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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
13/02/2015 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, D. A. F. da; COTTA, S. R.; VOLLÚ, R. E.; JURELEVICIUS, D. A. de; MARQUES, J. M.; MARRIEL, I. E.; SELDIN, L. |
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Afiliação: |
IVANILDO EVODIO MARRIEL, CNPMS. |
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Título: |
Endophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Microbiology, v. 14, n. 332, p. 1-9, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507. MenosBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed... Mostrar Tudo |
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Palavras-Chave: |
Enzima PAT; Proteína Cry. |
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Thesagro: |
Alimento transgênico; Milho. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02526naa a2200241 a 4500
001 2008692
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
100 1 $aFERREIRA, D. A. F. da
245 $aEndophytic microbial community in two transgenic maize genotypes and in their near-isogenic non-transgenic maize genotype.$h[electronic resource]
260 $c2014
520 $aBackground: Despite all the benefits assigned to the genetically modified plants, there are still no sufficient data available in literature concerning the possible effects on the microbial communities associated with these plants. Therefore, this study was aimed at examining the effects of the genetic modifications of two transgenic maize genotypes (MON810–expressing the insecticidal Bt-toxin and TC1507–expressing the insecticidal Bt-toxin and the herbicide resistance PAT [phosphinothricin-N -acetyltransferase]) on their endophytic microbial communities, in comparison to the microbial community found in the near-isogenic non-transgenic maize (control). Results: The structure of the endophytic communities (Bacteria, Archaea and fungi) and their composition (Bacteria) were evaluated by denaturing gradient gel electrophoresis (DGGE) and the construction of clone libraries, respectively. DGGE analysis and the clone libraries of the bacterial community showed that genotype TC1507 slightly differed from the other two genotypes. Genotype TC1507 showed a higher diversity within its endophytic bacterial community when compared to the other genotypes. Although some bacterial genera were found in all genotypes, such as the genera Burkholderia, Achromobacer and Stenotrophomonas, some were unique to genotype TC1507. Moreover, OTUs associated with Enterobacter predominated only in TC1507 clone libraries. Conclusion: The endophytic bacterial community of the maize genotype TC1507 differed from the communities of the maize genotype MON810 and of their near-isogenic parental genotypes (non-Bt or control). The differences observed among the maize genotypes studied may be associated with insertion of the gene coding for the protein PAT present only in the transgenic genotype TC1507.
650 $aAlimento transgênico
650 $aMilho
653 $aEnzima PAT
653 $aProteína Cry
700 1 $aCOTTA, S. R.
700 1 $aVOLLÚ, R. E.
700 1 $aJURELEVICIUS, D. A. de
700 1 $aMARQUES, J. M.
700 1 $aMARRIEL, I. E.
700 1 $aSELDIN, L.
773 $tBMC Microbiology$gv. 14, n. 332, p. 1-9, 2014.
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| 8. |  | ARRIEL, N. H. C.; SILVA, F. K. G. da; PINTO, S. M.; ARAÚJO, B. F.; DINIZ, A. L.; COUTINHO, T. de C.; OLIVEIRA, E. S. Caracterização morfo-agronômica de gergelim, cultivar BRS 196, submetido ao agente químico alquilante EMS. Campina Grande: Embrapa Algodão, 2007. 4 p. (Comunicado Técnico, 338)| Tipo: Comunicado Técnico/Recomendações Técnicas |
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