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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/02/2012 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, M. D. R.; VITOR, A. DE P.; CARNEIRO, J. da C.; PACIULLO, D. S. C.; MATOS, R. C.; MATOS, M. A. C. |
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Afiliação: |
MELLINA DAMASCENO RACHID SANTOS, UFJF; ALINE DE PAULA VITOR, UFJF; JAILTON DA COSTA CARNEIRO, CNPGL; DOMINGOS SAVIO CAMPOS PACIULLO, CNPGL; RENATO CAMARGO MATOS, UFJF; MARIA AUXILIADORA COSTA MATOS, UFJF. |
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Título: |
Use of ultrasound bath in the extraction and quantification of Ester-Linked Phenolic Acids in tropical forages. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
American Journal of Analytical Chemistry, v. 2, p. 344-351, 2011. |
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DOI: |
http://dx.doi.org/10.4236/ajac.2011.23042 |
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Idioma: |
Inglês |
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Conteúdo: |
A method was developed for the analysis of ester-linked phenolic acids in forage samples using extraction by an ultrasound-assisted treatment and quantification by HPLC with a UV-VIS detector. A reversed-phase C18 column was used for developing the method and the optimal condition was established with isocratic elution using acetonitrile/methanol/H3PO4 pH 2.08 (13:12.5:74.5) as the mobile phase. To reduce the time of sample processing, the extraction of ester-linked phenolic acids was studied using ultrasound bath and the results were then compared with those from an extraction usual using alkaline hydrolysis (20°C for 24 h). The method was valued through external and internal calibration. Internal calibration using o-coumaric acid as internal standard and m-coumaric acid as surrogate internal standard showed better results. The detection limits were of 0.09 and 0.04 mg●L-1 for p-coumaric and ferulic acids, respectively. The proposed method showed a good linear dynamic range (3.00 - 30.00 mg●L-1) for the analytes. The usefulness of the methodology was demonstrated by addition-recovery experiments using forage samples and values were in the 83 to 99% range. The extraction of ester-linked phenolic acids by 120 minutes of ultrasound bath was faster and more reproducible than alkaline hydrolysis (20°C for 24 h). |
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Palavras-Chave: |
Internal Calibration; Ultrasound Bath. |
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Thesaurus Nal: |
forage; phenolic acids. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02110naa a2200241 a 4500
001 1914719
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4236/ajac.2011.23042$2DOI
100 1 $aSANTOS, M. D. R.
245 $aUse of ultrasound bath in the extraction and quantification of Ester-Linked Phenolic Acids in tropical forages.$h[electronic resource]
260 $c2011
520 $aA method was developed for the analysis of ester-linked phenolic acids in forage samples using extraction by an ultrasound-assisted treatment and quantification by HPLC with a UV-VIS detector. A reversed-phase C18 column was used for developing the method and the optimal condition was established with isocratic elution using acetonitrile/methanol/H3PO4 pH 2.08 (13:12.5:74.5) as the mobile phase. To reduce the time of sample processing, the extraction of ester-linked phenolic acids was studied using ultrasound bath and the results were then compared with those from an extraction usual using alkaline hydrolysis (20°C for 24 h). The method was valued through external and internal calibration. Internal calibration using o-coumaric acid as internal standard and m-coumaric acid as surrogate internal standard showed better results. The detection limits were of 0.09 and 0.04 mg●L-1 for p-coumaric and ferulic acids, respectively. The proposed method showed a good linear dynamic range (3.00 - 30.00 mg●L-1) for the analytes. The usefulness of the methodology was demonstrated by addition-recovery experiments using forage samples and values were in the 83 to 99% range. The extraction of ester-linked phenolic acids by 120 minutes of ultrasound bath was faster and more reproducible than alkaline hydrolysis (20°C for 24 h).
650 $aforage
650 $aphenolic acids
653 $aInternal Calibration
653 $aUltrasound Bath
700 1 $aVITOR, A. DE P.
700 1 $aCARNEIRO, J. da C.
700 1 $aPACIULLO, D. S. C.
700 1 $aMATOS, R. C.
700 1 $aMATOS, M. A. C.
773 $tAmerican Journal of Analytical Chemistry$gv. 2, p. 344-351, 2011.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | SANTOS, M. D. R.; VITOR, A. de P.; CARNEIRO, J. da C.; PACIULLO, D. S. C.; MATOS, R. C.; MATOS, M. A. C. Desenvolvimento de um método de extração por ultra-som para a determinação de ácidos fenólicos em forrageiras tropicais. In: ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA, 15., 2009, Salvador. Anais... Salvador: SBQA, 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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