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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
19/07/2001 |
Data da última atualização: |
19/07/2001 |
Autoria: |
FONTES, R. R.; VILELA, N. J. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Status vegetable crops in Brazil and future perspectives. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON TIMING OF FIELD PRODUCTION IN VEGETABLE CROPS, 9., 2001, Piracicaba. Abstracts [and] program... Piracicaba: ISHS / SOB / ESALQ / Embrapa Vegetables / Embrapa Corn and Sorghum, 2001. |
Páginas: |
p.8. |
Idioma: |
Inglês |
Palavras-Chave: |
Brasil; Production. |
Thesagro: |
Hortaliça; Produção. |
Thesaurus Nal: |
Brazil; vegetables. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00683naa a2200205 a 4500 001 1769989 005 2001-07-19 008 2001 bl uuuu u00u1 u #d 100 1 $aFONTES, R. R. 245 $aStatus vegetable crops in Brazil and future perspectives. 260 $c2001 300 $ap.8. 650 $aBrazil 650 $avegetables 650 $aHortaliça 650 $aProdução 653 $aBrasil 653 $aProduction 700 1 $aVILELA, N. J. 773 $tIn: INTERNATIONAL SYMPOSIUM ON TIMING OF FIELD PRODUCTION IN VEGETABLE CROPS, 9., 2001, Piracicaba. Abstracts [and] program... Piracicaba: ISHS / SOB / ESALQ / Embrapa Vegetables / Embrapa Corn and Sorghum, 2001.
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Embrapa Hortaliças (CNPH) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
24/08/2010 |
Data da última atualização: |
26/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RETTEDAL, E.; VILAIN, S.; LINDBLOM, S.; LEHNERT, K.; SCOFIELD, C.; GEORGE, S.; CLAY, S.; KAUSHIK, R. S.; ROSA, A. J. de M.; FRANCIS, D.; BRÖZEL, V. S. |
Afiliação: |
ELIZABETH RETTEDAL, DEPARTMENTS OF BIOLOGY AND MICROBIOLOGY; SÉBASTIEN VILAIN, DEPARTMENTS OF BIOLOGY AND MICROBIOLOGY; STACY LINDBLOM, ANIMAL AND RANGE SCIENCE; KELLY LEHNERT, DEPARTMENTS OF BIOLOGY AND MICROBIOLOGY; CLAY SCOFIELD, DEPARTMENTS OF BIOLOGY AND MICROBIOLOGY; SAJAN GEORGE, DEPARTMENTS OF BIOLOGY AND MICROBIOLOGY; SHARON CLAY, PLANT SCIENCE; RADHEY S. KAUSHIK, CENTER FOR INFECTIONS DISEASE RESEARCH AND VACCINOLOGY; ARTUR JORDAO DE MAGALHAES ROSA, CPAC; DAVIS FRANCIS, VETERINARY SCIENCE; VOLKER S. BRÖZEL, DEPARTMENTS OF BIOLOGY AND MICROBIOLOGY. |
Título: |
Alteration of the ileal microbiota of weanling piglets by the growth-promoting antibiotic chlortetracycline. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 75, n. 17, p. 5489-5495, sept. 2009. |
DOI: |
10.1128/AEM.02220-08 |
Idioma: |
Inglês |
Conteúdo: |
Antibiotics such as chlortetracycline (CTC) have been used to promote growth of pigs for decades, but concerns over increased antibiotic-resistant infections in humans have prompted the development of alternative strategies. Developing alternatives to antibiotic growth promoters (AGPs) could be informed by information on the mechanisms of growth promotion, notably, how AGPs affect the microbial populations of the gastrointestinal tract. Pigs from three sows were aseptically delivered by cesarean section. Six piglets were distributed to each of two foster mothers until weaning, when piglets were fed a diet with or without 50 mg/kg CTC for 2 weeks. The ileal bacterial microbiota was characterized by using a cultivation-independent approach based on DNA extraction, PCR amplification, cloning, and sequencing of the 16S rRNA gene pool. The ileal and mucosal communities of these growing pigs were dominated by Lactobacillus bacteria, various members of the family Clostridiaceae, and members of the poorly known genus Turicibacter. Overall, CTC treatment resulted in three shifts: a decrease in Lactobacillus johnsonii, an increase in L. amylovorus, and a decrease in Turicibacter phylotypes. The composition of the microbiota varied considerably between individual pigs, as revealed by shared operational taxonomic units (OTUs) and similarity (SONS) analysis (theta(YC) values). While the observed variation between untreated pigs obscured the possible effect of CTC, integral-LIBSHUFF and SONS analyses of pooled libraries indicated a significant shift due to CTC in both the lumen and the mucosa, with some OTUs unique to either treated or control ileum. DOTUR analysis revealed little overlap between control and treated communities at the 3% difference level, indicating unique ileal communities in the presence of CTC. MenosAntibiotics such as chlortetracycline (CTC) have been used to promote growth of pigs for decades, but concerns over increased antibiotic-resistant infections in humans have prompted the development of alternative strategies. Developing alternatives to antibiotic growth promoters (AGPs) could be informed by information on the mechanisms of growth promotion, notably, how AGPs affect the microbial populations of the gastrointestinal tract. Pigs from three sows were aseptically delivered by cesarean section. Six piglets were distributed to each of two foster mothers until weaning, when piglets were fed a diet with or without 50 mg/kg CTC for 2 weeks. The ileal bacterial microbiota was characterized by using a cultivation-independent approach based on DNA extraction, PCR amplification, cloning, and sequencing of the 16S rRNA gene pool. The ileal and mucosal communities of these growing pigs were dominated by Lactobacillus bacteria, various members of the family Clostridiaceae, and members of the poorly known genus Turicibacter. Overall, CTC treatment resulted in three shifts: a decrease in Lactobacillus johnsonii, an increase in L. amylovorus, and a decrease in Turicibacter phylotypes. The composition of the microbiota varied considerably between individual pigs, as revealed by shared operational taxonomic units (OTUs) and similarity (SONS) analysis (theta(YC) values). While the observed variation between untreated pigs obscured the possible effect of CTC, integral-LIBSHUFF and S... Mostrar Tudo |
Palavras-Chave: |
Clortetraciclina. |
Thesagro: |
Antibiótico; Infecção. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02661naa a2200289 a 4500 001 1860768 005 2019-04-26 008 2009 bl --- 0-- u #d 024 7 $a10.1128/AEM.02220-08$2DOI 100 1 $aRETTEDAL, E. 245 $aAlteration of the ileal microbiota of weanling piglets by the growth-promoting antibiotic chlortetracycline. 260 $c2009 520 $aAntibiotics such as chlortetracycline (CTC) have been used to promote growth of pigs for decades, but concerns over increased antibiotic-resistant infections in humans have prompted the development of alternative strategies. Developing alternatives to antibiotic growth promoters (AGPs) could be informed by information on the mechanisms of growth promotion, notably, how AGPs affect the microbial populations of the gastrointestinal tract. Pigs from three sows were aseptically delivered by cesarean section. Six piglets were distributed to each of two foster mothers until weaning, when piglets were fed a diet with or without 50 mg/kg CTC for 2 weeks. The ileal bacterial microbiota was characterized by using a cultivation-independent approach based on DNA extraction, PCR amplification, cloning, and sequencing of the 16S rRNA gene pool. The ileal and mucosal communities of these growing pigs were dominated by Lactobacillus bacteria, various members of the family Clostridiaceae, and members of the poorly known genus Turicibacter. Overall, CTC treatment resulted in three shifts: a decrease in Lactobacillus johnsonii, an increase in L. amylovorus, and a decrease in Turicibacter phylotypes. The composition of the microbiota varied considerably between individual pigs, as revealed by shared operational taxonomic units (OTUs) and similarity (SONS) analysis (theta(YC) values). While the observed variation between untreated pigs obscured the possible effect of CTC, integral-LIBSHUFF and SONS analyses of pooled libraries indicated a significant shift due to CTC in both the lumen and the mucosa, with some OTUs unique to either treated or control ileum. DOTUR analysis revealed little overlap between control and treated communities at the 3% difference level, indicating unique ileal communities in the presence of CTC. 650 $aAntibiótico 650 $aInfecção 653 $aClortetraciclina 700 1 $aVILAIN, S. 700 1 $aLINDBLOM, S. 700 1 $aLEHNERT, K. 700 1 $aSCOFIELD, C. 700 1 $aGEORGE, S. 700 1 $aCLAY, S. 700 1 $aKAUSHIK, R. S. 700 1 $aROSA, A. J. de M. 700 1 $aFRANCIS, D. 700 1 $aBRÖZEL, V. S. 773 $tApplied and Environmental Microbiology$gv. 75, n. 17, p. 5489-5495, sept. 2009.
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