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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/07/2018 |
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Data da última atualização: |
10/07/2018 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
SOUZA, F. V. D.; SOUZA, E. H. de; KAYA, E.; VIEIRA, L. de J.; SILVA, R. L. da. |
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Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA; ERGUN KAYA; LÍVIA DE JESUS VIEIRA; RONILZE LEITE DA SILVA. |
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Título: |
Cryopreservation of pineapple Shoot Tips by the droplet vitrification technique. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018. |
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Páginas: |
269-277 |
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Descrição Física: |
v.1815. Cap. 18. |
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DOI: |
.1007/978-1-4939-8594-4_18 |
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Idioma: |
Inglês |
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Conteúdo: |
Cryopreservation is a technique that allows the conservation of many species for long periods. Among the protocols used for cryopreservation, droplet vitrification has shown efficient results in preserving shoot tips of various wild and cultivated pineapple genotypes. The method consists of extraction of shoot tips from plants grown in vitro, dehydration for a period of 48 h in a preculture medium supplemented with a high concentration of sucrose, treatment in a plant vitrification solution (PVS2), and immersion in liquid nitrogen. The method described in this chapter has produced survival and regeneration indices of around 70%, depending on the genotype and physiological conditions of the initial explants. The objective of this chapter is to describe in detail a droplet vitrification protocol for shoot tips that is easy to perform for cryopreservation of pineapple germplasm. |
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Thesagro: |
Criopreservação. |
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Thesaurus Nal: |
Conservation plants; Solutions; Vitrification. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $a.1007/978-1-4939-8594-4_18$2DOI
100 1 $aSOUZA, F. V. D.
245 $aCryopreservation of pineapple Shoot Tips by the droplet vitrification technique.$h[electronic resource]
260 $c2018
300 $a269-277$cv.1815. Cap. 18.
520 $aCryopreservation is a technique that allows the conservation of many species for long periods. Among the protocols used for cryopreservation, droplet vitrification has shown efficient results in preserving shoot tips of various wild and cultivated pineapple genotypes. The method consists of extraction of shoot tips from plants grown in vitro, dehydration for a period of 48 h in a preculture medium supplemented with a high concentration of sucrose, treatment in a plant vitrification solution (PVS2), and immersion in liquid nitrogen. The method described in this chapter has produced survival and regeneration indices of around 70%, depending on the genotype and physiological conditions of the initial explants. The objective of this chapter is to describe in detail a droplet vitrification protocol for shoot tips that is easy to perform for cryopreservation of pineapple germplasm.
650 $aConservation plants
650 $aSolutions
650 $aVitrification
650 $aCriopreservação
700 1 $aSOUZA, E. H. de
700 1 $aKAYA, E.
700 1 $aVIEIRA, L. de J.
700 1 $aSILVA, R. L. da
773 $tIn: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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