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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
21/08/2018 |
Data da última atualização: |
22/08/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIEIRA, A. F.; ALMEIDA, L. C. S.; RODRIGUES, L. A.; COSTA, J. G. C.; MELO, L. C.; PEREIRA, H. S.; SANGLARD, D. A.; SOUZA, T. L. P. O. |
Afiliação: |
A. F. VIEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; L. C. S. ALMEIDA, UNIVERSIDADE FEDERAL DE GOIÁS; LUANA ALVES RODRIGUES, CNPAF; JOAQUIM GERALDO CAPRIO DA COSTA, CNPAF; LEONARDO CUNHA MELO, CNPAF; HELTON SANTOS PEREIRA, CNPAF; D. A. SANGLARD, UNIVERSIDADE FEDERAL DE MINAS GERAIS, Montes Claros-MG; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF. |
Título: |
Selection of resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 17, gmr18066, Mar. 2018. |
ISSN: |
1676-5680 |
DOI: |
10.4238/gmr18066 |
Idioma: |
Inglês |
Conteúdo: |
The main goal of this work was to select resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Fifty-five common bean genotypes, including differential varieties, characterized resistance sources, elite lines, cultivars and controls, were evaluated in a field inoculation trial and screened with SCAR markers linked to resistance genes that are important in Brazil. The field trial was carried out in Santo Antônio de Goiás, GO, Brazil, during the fall/winter growing season of 2014, using artificial inoculation with a mixture of six races of Colletotrichum lindemuthianum, selected based on their high virulence and prevalence in Brazil. Amplification reactions with the SCAR markers previously identified as linked to important anthracnose resistance genes on Brazil followed standard procedures. Twenty-eight of the 58 genotypes were resistant to anthracnose (mean severity score ≤ 3.5). Ten of these 28 resistant genotypes stood out because they presented a mean anthracnose severity score of 1.0. Four of the six SCAR markers tested shown to be useful for the assisted selection of their respective target genes (SH18 and SAS13 for Co-42, SAB03 for Co-5, and SAZ20 for Co-6). Two carioca seeded elite lines were highlighted by the phenotypic and molecular screening: K10 (Co-34, Co-42, Co-5 and Co-6) and K13 (Co-4²). The phenotypic and molecular characterization of candidate resistance sources to common bean anthracnose based on their disease reaction in field inoculation trials and on the analysis with molecular markers linked to resistance genes has shown to be a useful strategy. These results aid in the selection of donor parents and resistant lines to be preferably explored by common bean breeding programs in Brazil. MenosThe main goal of this work was to select resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Fifty-five common bean genotypes, including differential varieties, characterized resistance sources, elite lines, cultivars and controls, were evaluated in a field inoculation trial and screened with SCAR markers linked to resistance genes that are important in Brazil. The field trial was carried out in Santo Antônio de Goiás, GO, Brazil, during the fall/winter growing season of 2014, using artificial inoculation with a mixture of six races of Colletotrichum lindemuthianum, selected based on their high virulence and prevalence in Brazil. Amplification reactions with the SCAR markers previously identified as linked to important anthracnose resistance genes on Brazil followed standard procedures. Twenty-eight of the 58 genotypes were resistant to anthracnose (mean severity score ≤ 3.5). Ten of these 28 resistant genotypes stood out because they presented a mean anthracnose severity score of 1.0. Four of the six SCAR markers tested shown to be useful for the assisted selection of their respective target genes (SH18 and SAS13 for Co-42, SAB03 for Co-5, and SAZ20 for Co-6). Two carioca seeded elite lines were highlighted by the phenotypic and molecular screening: K10 (Co-34, Co-42, Co-5 and Co-6) and K13 (Co-4²). The phenotypic and molecular characterization of candidate resistance sources to common bean anthracnose based on their d... Mostrar Tudo |
Thesagro: |
Antracnose; DNA; Feijão; Marcador Molecular; Phaseolus Vulgaris; Resistência Genética. |
Thesaurus Nal: |
Anthracnose; Beans; Genetic resistance; Marker-assisted selection; Plant breeding. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181721/1/CNPAF-2018-gmr1.pdf
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Marc: |
LEADER 02840naa a2200361 a 4500 001 2094367 005 2018-08-22 008 2018 bl uuuu u00u1 u #d 022 $a1676-5680 024 7 $a10.4238/gmr18066$2DOI 100 1 $aVIEIRA, A. F. 245 $aSelection of resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening.$h[electronic resource] 260 $c2018 520 $aThe main goal of this work was to select resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Fifty-five common bean genotypes, including differential varieties, characterized resistance sources, elite lines, cultivars and controls, were evaluated in a field inoculation trial and screened with SCAR markers linked to resistance genes that are important in Brazil. The field trial was carried out in Santo Antônio de Goiás, GO, Brazil, during the fall/winter growing season of 2014, using artificial inoculation with a mixture of six races of Colletotrichum lindemuthianum, selected based on their high virulence and prevalence in Brazil. Amplification reactions with the SCAR markers previously identified as linked to important anthracnose resistance genes on Brazil followed standard procedures. Twenty-eight of the 58 genotypes were resistant to anthracnose (mean severity score ≤ 3.5). Ten of these 28 resistant genotypes stood out because they presented a mean anthracnose severity score of 1.0. Four of the six SCAR markers tested shown to be useful for the assisted selection of their respective target genes (SH18 and SAS13 for Co-42, SAB03 for Co-5, and SAZ20 for Co-6). Two carioca seeded elite lines were highlighted by the phenotypic and molecular screening: K10 (Co-34, Co-42, Co-5 and Co-6) and K13 (Co-4²). The phenotypic and molecular characterization of candidate resistance sources to common bean anthracnose based on their disease reaction in field inoculation trials and on the analysis with molecular markers linked to resistance genes has shown to be a useful strategy. These results aid in the selection of donor parents and resistant lines to be preferably explored by common bean breeding programs in Brazil. 650 $aAnthracnose 650 $aBeans 650 $aGenetic resistance 650 $aMarker-assisted selection 650 $aPlant breeding 650 $aAntracnose 650 $aDNA 650 $aFeijão 650 $aMarcador Molecular 650 $aPhaseolus Vulgaris 650 $aResistência Genética 700 1 $aALMEIDA, L. C. S. 700 1 $aRODRIGUES, L. A. 700 1 $aCOSTA, J. G. C. 700 1 $aMELO, L. C. 700 1 $aPEREIRA, H. S. 700 1 $aSANGLARD, D. A. 700 1 $aSOUZA, T. L. P. O. 773 $tGenetics and Molecular Research$gv. 17, gmr18066, Mar. 2018.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
17/03/2004 |
Data da última atualização: |
12/01/2009 |
Autoria: |
FARIA, L. C. de; DEL PELOSO, M. J.; COSTA, J. G. C. da; RAVA, C. A.; CABRERA DIAZ, J. L.; MELO, L. C.; SOARES, D. M.; CARNEIRO, G. E. de S. |
Afiliação: |
Embrapa Arroz e Feijão. |
Título: |
Relatório: resultado dos ensaios de valor de cultivo e uso - 2001/2002 e ensaios nacionais - 2001. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2003. |
Páginas: |
251 p. |
Série: |
(Embrapa Arroz e Feijão. Documentos. 150). |
Idioma: |
Português |
Conteúdo: |
No presente relatório são apresentados os resultados dos ensaios da etapa de avaliação do biênio 2001/2002. |
Palavras-Chave: |
Melhoramento genético. |
Thesagro: |
Feijão; Pesquisa; Phaseolus Vulgaris. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAF/21049/1/doc_150.pdf
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Marc: |
LEADER 00906nam a2200265 a 4500 001 1211970 005 2009-01-12 008 2003 bl uuuu 00u1 u #d 100 1 $aFARIA, L. C. de 245 $aRelatório$bresultado dos ensaios de valor de cultivo e uso - 2001/2002 e ensaios nacionais - 2001. 260 $aSanto Antônio de Goiás: Embrapa Arroz e Feijão$c2003 300 $a251 p. 490 $a(Embrapa Arroz e Feijão. Documentos. 150). 520 $aNo presente relatório são apresentados os resultados dos ensaios da etapa de avaliação do biênio 2001/2002. 650 $aFeijão 650 $aPesquisa 650 $aPhaseolus Vulgaris 653 $aMelhoramento genético 700 1 $aDEL PELOSO, M. J. 700 1 $aCOSTA, J. G. C. da 700 1 $aRAVA, C. A. 700 1 $aCABRERA DIAZ, J. L. 700 1 $aMELO, L. C. 700 1 $aSOARES, D. M. 700 1 $aCARNEIRO, G. E. de S.
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