Data/hora: 14/06/2024 00:31:25

Biblioteca(s): | Embrapa Agricultura Digital; Embrapa Café.
Data corrente: | 05/02/2015
Data da última atualização: | 06/02/2015
Autoria: | CARDOSO, D. C.; MARTINATI, J. C.; GIACHETTO, P. F.; VIDAL, R. O.; CARAZZOLLE, M. F.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P.
Afiliação: | DANIELLE C. CARDOSO, IAC; JULIANA C MARTINATI, IAC; POLIANA FERNANDA GIACHETTO, CNPTIA; RAMON O. VIDAL; MARCELO F. CARAZZOLLE; LILIAN PADILHA, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC; MIRIAN PEREZ MALUF, SAPC.
Título: | Large-scale analysis of differential gene expression in coffee genotypes resistant and susceptible to leaf miner-toward the identification of candidate genes for marker assisted-selection.
Ano de publicação: | 2014
Fonte/Imprenta: | BMC Genomics, London, v. 15, p. 1-20, 2014.
DOI: | 10.1186/1471-2164-15-66
Idioma: | Inglês
Conteúdo: | A successful development of herbivorous insects into plant tissues depends on coordination of metabolic processes. Plants have evolved complex mechanisms to recognize such attacks, and to trigger a defense response. To understand the transcriptional basis of this response, we compare gene expression profiles of two coffee genotypes, susceptible and resistant to leaf miner (Leucoptera coffella). A total of 22000 EST sequences from the Coffee Genome Database were selected for a microarray analysis. Fluorescence probes were synthesized using mRNA from the infested and non-infested coffee plants. Array hybridization, scanning and data normalization were performed using Nimble Scan® e ArrayStar® platforms. Genes with foldchange values +/-2 were considered differentially expressed. A validation of 18 differentially expressed genes was performed in infected plants using qRT-PCR approach.
Palavras-Chave: | Microarranjo; Plant defense.
Thesagro: | Café; Coffea Arábica.
Thesaurus Nal: | Genes; Leafminers; Microarray technology.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/117310/1/Large-scale-analysis.pdf
Registro original: | Embrapa Agricultura Digital (CNPTIA)

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