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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
04/01/2021 |
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Data da última atualização: |
08/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C. M. dos; NASCIMENTO, W. B. A.; NASCIMENRO, B. P. do; SCHWAB, S.; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
Carlos M. Dos Santos, UFRRJ; Wiglison B. A. Nascimento, UFRRK; Bruna P. do Nascimento, UFRRJ; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
Temporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Microbiological Research, v. 244, e126651, 2021. |
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DOI: |
10.1016/j.micres.2020.126651 |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacetobacter diazotrophicus is a species of great agronomic potential due to its growth-promotion traits. Its colonization process in different plants has been reported. However, there have been no studies regarding its structural colonization in elephant grass. This is a fast-growing C4-Poaceae plant, and its application in Brazil is mainly aimed at feeding dairy cattle, due to its high nutritional value. Also, in the last decade, this grass has been applied in the production of biofuels. The present study aimed to monitor the colonization process of strain LP343 of G. diazotrophicus inoculated in elephant grass seedlings of PCEA genotype, by using a mCherry-tagged bacterium. Samples of roots and shoots collected at different periods were visualized by confocal laser-scanning microscopy. The colony-counting assay was used to compare the number of cells recovered in different niches and a qPCR was performed for the quantification of endophytic cells in root and shoot tissues. Results suggested that the strain LP343 quickly recognized the PCEA roots as host, attached to the elephant grass roots at 6 h, and 7 days after inoculation were able to colonize the xylem vessels of roots and shoots of elephant grass. This study advances our knowledge about the colonization process of G. diazotrophicus species in elephant grass, contributing to future studies involving the plant-bacteria interaction cultivated under gnotobiotic conditions. |
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Palavras-Chave: |
Endophytic colonization; FBN. |
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Thesaurus Nal: |
Genotype. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02215naa a2200229 a 4500
001 2128964
005 2021-01-08
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.micres.2020.126651$2DOI
100 1 $aSANTOS, C. M. dos
245 $aTemporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343.$h[electronic resource]
260 $c2021
520 $aGluconacetobacter diazotrophicus is a species of great agronomic potential due to its growth-promotion traits. Its colonization process in different plants has been reported. However, there have been no studies regarding its structural colonization in elephant grass. This is a fast-growing C4-Poaceae plant, and its application in Brazil is mainly aimed at feeding dairy cattle, due to its high nutritional value. Also, in the last decade, this grass has been applied in the production of biofuels. The present study aimed to monitor the colonization process of strain LP343 of G. diazotrophicus inoculated in elephant grass seedlings of PCEA genotype, by using a mCherry-tagged bacterium. Samples of roots and shoots collected at different periods were visualized by confocal laser-scanning microscopy. The colony-counting assay was used to compare the number of cells recovered in different niches and a qPCR was performed for the quantification of endophytic cells in root and shoot tissues. Results suggested that the strain LP343 quickly recognized the PCEA roots as host, attached to the elephant grass roots at 6 h, and 7 days after inoculation were able to colonize the xylem vessels of roots and shoots of elephant grass. This study advances our knowledge about the colonization process of G. diazotrophicus species in elephant grass, contributing to future studies involving the plant-bacteria interaction cultivated under gnotobiotic conditions.
650 $aGenotype
653 $aEndophytic colonization
653 $aFBN
700 1 $aNASCIMENTO, W. B. A.
700 1 $aNASCIMENRO, B. P. do
700 1 $aSCHWAB, S.
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tMicrobiological Research$gv. 244, e126651, 2021.
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Embrapa Agrobiologia (CNPAB) |
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