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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
ABUD, S.; SOUZA, P. I. M.; MOREIRA, C. T.; FARIAS NETO, A. L.; VIANNA, G. R.; ANDRADE, S. R. M.; NUNES JÚNIOR, J.; GUERZONI, R. A.; MONTEIRO, P. M. F. O.; ASSUNÇÃO, M. S.; RECH, E. L.; ARAGÃO, F. J. L. |
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Título: |
Distance of flow in transgenic BROO-69515 and the non transgenic soybean in the Cerrado Region, Brazil. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 110-111. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
In order to evaluate the gene flow from the transgenic soybean RR (Round Up ready) to non-transgenic soybean, an experiment was conducted in Embrapa Cerrados (Federal District, Brazil). Plots with non-transgenic plants, were sowed around a central plot with the transgenic soybean plants (Var. Conquista, line BR00-69515), transformed with the cp4 epsps gene, which confers glifosate resistance. The plots were previously identified and the seeds of each plot were separately harvested, counted and sowed to evaluate the resistant plants containing the cp4 epsps gene. Plants were sprayed at the third-trifoliate stage with the herbicide glifosate (Round Up) at 2,4 Kg.ha-1. Plants that survived were analyzed by PCR. The percentage of natural cross was calculated as the fraction of tolerant plants from the total number of plants. The frequency of cross-pollination between the transgenic line BR 00-69515 and non-transgenic plants were 0,485% (at 1 meter from the central plot). The frequency of cross pollination was dramatically reduced at 2 m from the central plot (0,099 %), and reduced to 0.063%, 0.054 %, 0.021 %, 0.019 %, 0.020 %, 0.009 %, 0.006 % e 0.005 % in each subsequent meter, up to 10 m of the central plot. |
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Categoria do assunto: |
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Marc: |
LEADER 02419naa a2200289 a 4500
001 1466774
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aABUD, S.
245 $aDistance of flow in transgenic BROO-69515 and the non transgenic soybean in the Cerrado Region, Brazil.
260 $c2004
300 $ap. 110-111.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aIn order to evaluate the gene flow from the transgenic soybean RR (Round Up ready) to non-transgenic soybean, an experiment was conducted in Embrapa Cerrados (Federal District, Brazil). Plots with non-transgenic plants, were sowed around a central plot with the transgenic soybean plants (Var. Conquista, line BR00-69515), transformed with the cp4 epsps gene, which confers glifosate resistance. The plots were previously identified and the seeds of each plot were separately harvested, counted and sowed to evaluate the resistant plants containing the cp4 epsps gene. Plants were sprayed at the third-trifoliate stage with the herbicide glifosate (Round Up) at 2,4 Kg.ha-1. Plants that survived were analyzed by PCR. The percentage of natural cross was calculated as the fraction of tolerant plants from the total number of plants. The frequency of cross-pollination between the transgenic line BR 00-69515 and non-transgenic plants were 0,485% (at 1 meter from the central plot). The frequency of cross pollination was dramatically reduced at 2 m from the central plot (0,099 %), and reduced to 0.063%, 0.054 %, 0.021 %, 0.019 %, 0.020 %, 0.009 %, 0.006 % e 0.005 % in each subsequent meter, up to 10 m of the central plot.
700 1 $aSOUZA, P. I. M.
700 1 $aMOREIRA, C. T.
700 1 $aFARIAS NETO, A. L.
700 1 $aVIANNA, G. R.
700 1 $aANDRADE, S. R. M.
700 1 $aNUNES JÚNIOR, J.
700 1 $aGUERZONI, R. A.
700 1 $aMONTEIRO, P. M. F. O.
700 1 $aASSUNÇÃO, M. S.
700 1 $aRECH, E. L.
700 1 $aARAGÃO, F. J. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MARTINS, G. R.; MARINHO, R. C.; BEZERRA-JUNIOR, R. Q.; CÂMARA, L. M. C.; ALBUQUERQUE-PINTO, L. C.; TEIXEIRA, M. F. S. |
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Afiliação: |
Gabrielle R. Martins, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Rebeca C. Marinho, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Rosivaldo Q. Bezerra-Junior, Laboratório de Imunologia/Programa de Pós-Graduação em Microbiologia Médica/Universidade Federal do Ceará; Lilia M. C. Câmara, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Luiz C. Albuquerque-Pinto, Laboratório de Imunologia/Programa de Pós-Graduação em Microbiologia Médica/Universidade Federal do Ceará; Maria F. S. Teixeira, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará. |
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Título: |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 643-649, jun. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino. |
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Conteúdo: |
Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood. |
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Palavras-Chave: |
Células tronco; Citometria de fluxo; Diferenciação em linhagenm; Lineage differentiation; MSC; Stem cell. |
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Thesaurus NAL: |
Flow cytometry; Goats. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163981/1/Isolation-culture-and-characterization-1.pdf
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Marc: |
LEADER 02331naa a2200289 a 4500
001 2075898
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMARTINS, G. R.
245 $aIsolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino.
520 $aMesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood.
650 $aFlow cytometry
650 $aGoats
653 $aCélulas tronco
653 $aCitometria de fluxo
653 $aDiferenciação em linhagenm
653 $aLineage differentiation
653 $aMSC
653 $aStem cell
700 1 $aMARINHO, R. C.
700 1 $aBEZERRA-JUNIOR, R. Q.
700 1 $aCÂMARA, L. M. C.
700 1 $aALBUQUERQUE-PINTO, L. C.
700 1 $aTEIXEIRA, M. F. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 6, p. 643-649, jun. 2017.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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