Data/hora: 12/06/2024 07:42:20

Biblioteca(s): 	Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	12/12/2018	
Data da última atualização: 	24/01/2023	
Autoria: 	WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M.	
Afiliação: 	S. WOHLRES-VIANA, UFJF; E. K. N. ARASHIRO, UFF; T. P. MINARE, Universidade José do Rosario Vellano, Alfenas, MG; C. A. C. FERNANDES, Universidade Jose do Rosario Vellano / Biotran Biotecnologia Animal, Alfenas, MG; J. G. V. GRAZIA, UFMG; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen.	
Título: 	Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	Theriogenology, v. 126, p. 68-74, 2019.	
DOI: 	10.1016/j.theriogenology.2018.12.004	
Idioma: 	Inglês	
Conteúdo: 	Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3&#8239;±&#8239;1.2 ova/embryos per flush, n&#8239;=&#8239;5) or poor responders (1.1&#8239;±&#8239;0.3 ova/embryos per flush, n&#8239;=&#8239;5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8&#8239;mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype.	
Palavras-Chave: 	In vivo embryo production.	
Thesaurus Nal: 	Embryo transfer; Luteinizing hormone.	
Categoria do assunto: 	--
L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CENARGEN - (37494-1) - UPC - AP - DD -  -  -  - DIS
CNPGL - (24514-1) - UPC - AP - DD -  -  -  - DIS